BBa_J176012 1 K Kozak 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 yeast/ human Kozak plus start codon false false _863_ 0 10865 9 In stock false TBA false Brady Laughlin BBa_J176041 1 scar Silver scar 2011-10-04T11:00:00Z 2015-08-31T04:08:34Z This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard). Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard). false false _863_ 0 1144 61 Not in stock false The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts. false Karmella Haynes BBa_J176050 1 BBa_J176050 miRNA CFP-reporter 1 2011-10-07T11:00:00Z 2015-08-31T04:08:34Z TBA miRNA CFP-reporter 1 false false _863_ 0 10865 9 Not in stock false TBA false Brady Laughlin component2157202 1 BBa_J176041 component2157217 1 BBa_S04704 component2157201 1 BBa_S04642 annotation2157201 1 BBa_S04642 range2157201 1 1 467 annotation2157202 1 BBa_J176041 range2157202 1 468 473 annotation2157217 1 BBa_S04704 range2157217 1 474 2937 BBa_S04642 1 BBa_S04642 J176003:J176019 2011-10-03T11:00:00Z 2015-05-08T01:14:43Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2154771 1 BBa_J176019 component2154770 1 BBa_J176041 component2154769 1 BBa_J176003 annotation2154769 1 BBa_J176003 range2154769 1 1 368 annotation2154771 1 BBa_J176019 range2154771 1 375 467 annotation2154770 1 BBa_J176041 range2154770 1 369 374 BBa_S04704 1 BBa_S04704 J176012:J176018 2011-10-06T11:00:00Z 2015-05-08T01:14:44Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2156825 1 BBa_J176042 component2156818 1 BBa_J176022 component2156812 1 BBa_J176012 component2156819 1 BBa_J176041 component2156823 1 BBa_J176041 component2156822 1 BBa_J176016 component2156814 1 BBa_J176018 component2156815 1 BBa_J176041 component2156821 1 BBa_J176041 component2156820 1 BBa_J176008 component2156816 1 BBa_J176022 component2156817 1 BBa_J176041 component2156813 1 BBa_J176041 annotation2156823 1 BBa_J176041 range2156823 1 1991 1996 annotation2156820 1 BBa_J176008 range2156820 1 1951 1971 annotation2156822 1 BBa_J176016 range2156822 1 1978 1990 annotation2156821 1 BBa_J176041 range2156821 1 1972 1977 annotation2156819 1 BBa_J176041 range2156819 1 1945 1950 annotation2156818 1 BBa_J176022 range2156818 1 1258 1944 annotation2156816 1 BBa_J176022 range2156816 1 565 1251 annotation2156815 1 BBa_J176041 range2156815 1 559 564 annotation2156825 1 BBa_J176042 range2156825 1 1997 2464 annotation2156814 1 BBa_J176018 range2156814 1 25 558 annotation2156817 1 BBa_J176041 range2156817 1 1252 1257 annotation2156812 1 BBa_J176012 range2156812 1 1 18 annotation2156813 1 BBa_J176041 range2156813 1 19 24 BBa_J176016 1 STOP STOP 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA contains stop codons TAA and TGA in frame false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176003 1 Ubc Ubc 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Ubc constitutive promoter false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176022 1 AmCyan AmCyan 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA cyan fluorescent protein from PamCyan-1C1 false false _863_ 0 10865 9 It's complicated false TBA false Karmella Haynes BBa_J176019 1 5xGal4 5xGal4 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA target sequence of GAL4 DB; PCR cloned from pNEB false false _863_ 0 10865 9 Not in stock false TBA false Brady Laughlin annotation2159561 1 Gal4 range2159561 1 39 55 annotation2159563 1 Gal4 range2159563 1 77 93 annotation2159562 1 Gal4 range2159562 1 58 74 annotation2159560 1 Gal4 range2159560 1 20 36 annotation2159559 1 Gal4 range2159559 1 1 17 BBa_J176008 1 NLS NLS 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 SV40 nuclear localization sequence false false _863_ 0 10865 9 In stock false TBA false Karmella Haynes BBa_J176018 1 PEST PEST 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA degradation tag false false _863_ 0 10865 9 It's complicated false TBA false Karmella Haynes BBa_J176042 1 polyA polyA 2011-10-06T11:00:00Z 2015-08-31T04:08:34Z Originally from the Silver Lab BioBrick collection. Biological source unknown. This part was created by David Drubin. Polyadenylation signal for mammalian genes false false _863_ 0 1144 61 Not in stock false This is the first version of polyA that we used to build many of our mammalian parts, until we switched to a streamlined, shorter polyA (polyA2, BBa_J176009). false Karmella Haynes annotation2156673 1 questionable base call range2156673 1 128 128 BBa_J176016_sequence 1 gcgtaaagctgag BBa_J176012_sequence 1 cccgccgccaccatggag BBa_J176042_sequence 1 agacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagctt BBa_J176018_sequence 1 gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata BBa_J176003_sequence 1 aaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggag BBa_J176050_sequence 1 aaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggagactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacccgccgccaccatggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagaagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagctt BBa_J176022_sequence 1 atggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttc BBa_S04704_sequence 1 cccgccgccaccatggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagaagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagctt BBa_J176041_sequence 1 actaga BBa_J176019_sequence 1 cggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccg BBa_S04642_sequence 1 aaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggagactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccg BBa_J176008_sequence 1 cccaagaaaaagcgcaaggta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z