BBa_J176050
type: ComponentDefinition
persistent identity: BBa J176050
display id: BBa_J176050
version: 1
was generated by: iGEM to SBOL conversion
description: miRNA CFP-reporter 1
created: 2011-10-07T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: miRNA CFP-reporter 1
discontinued: false
dominant: false
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_J176041
component: BBa_S04642
component: BBa_S04704
sequence annotation: BBa_S04642
sequence annotation: BBa_J176041
sequence annotation: BBa_S04704
sequence: https://synbiohub.org/public/igem/BBa_J176050_sequence/1

BBa_J176041
type: Component
persistent identity: component2157202
display id: component2157202
version: 1
top level: BBa_J176050
definition: scar
access: public

BBa_S04642
type: Component
persistent identity: component2157201
display id: component2157201
version: 1
top level: BBa_J176050
definition: BBa_S04642
access: public

BBa_S04704
type: Component
persistent identity: component2157217
display id: component2157217
version: 1
top level: BBa_J176050
definition: BBa_S04704
access: public

BBa_S04642
type: SequenceAnnotation
persistent identity: annotation2157201
display id: annotation2157201
version: 1
top level: BBa_J176050
location: https://synbiohub.org/public/igem/BBa_J176050/annotation2157201/range2157201/1
component: BBa_S04642

https://synbiohub.org/public/igem/BBa_J176050/annotation2157201/range2157201/1
type: Range
persistent identity: range2157201
display id: range2157201
version: 1
top level: BBa_J176050
direction: direction/reverse
start: 1
end: 467
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2157202
display id: annotation2157202
version: 1
top level: BBa_J176050
location: https://synbiohub.org/public/igem/BBa_J176050/annotation2157202/range2157202/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_J176050/annotation2157202/range2157202/1
type: Range
persistent identity: range2157202
display id: range2157202
version: 1
top level: BBa_J176050
direction: direction/reverse
start: 468
end: 473
orientation: inline

BBa_S04704
type: SequenceAnnotation
persistent identity: annotation2157217
display id: annotation2157217
version: 1
top level: BBa_J176050
location: https://synbiohub.org/public/igem/BBa_J176050/annotation2157217/range2157217/1
component: BBa_S04704

https://synbiohub.org/public/igem/BBa_J176050/annotation2157217/range2157217/1
type: Range
persistent identity: range2157217
display id: range2157217
version: 1
top level: BBa_J176050
direction: direction/reverse
start: 474
end: 2937
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa J176041
display id: BBa_J176041
version: 1
was generated by: iGEM to SBOL conversion
description: Silver scar
created: 2011-10-04T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard).
owned by: james
owned by: myers
mutable description: Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard).
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts.
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J176041_sequence/1

BBa_S04642
type: ComponentDefinition
persistent identity: BBa S04642
display id: BBa_S04642
version: 1
was generated by: iGEM to SBOL conversion
description: J176003:J176019
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:14:43Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J176019
component: BBa_J176041
component: BBa_J176003
sequence annotation: BBa_J176003
sequence annotation: BBa_J176041
sequence annotation: BBa_J176019
sequence: https://synbiohub.org/public/igem/BBa_S04642_sequence/1

BBa_J176019
type: Component
persistent identity: component2154771
display id: component2154771
version: 1
top level: BBa_S04642
definition: 5xGal4
access: public

BBa_J176041
type: Component
persistent identity: component2154770
display id: component2154770
version: 1
top level: BBa_S04642
definition: scar
access: public

BBa_J176003
type: Component
persistent identity: component2154769
display id: component2154769
version: 1
top level: BBa_S04642
definition: Ubc
access: public

BBa_J176003
type: SequenceAnnotation
persistent identity: annotation2154769
display id: annotation2154769
version: 1
top level: BBa_S04642
location: https://synbiohub.org/public/igem/BBa_S04642/annotation2154769/range2154769/1
component: BBa_J176003

https://synbiohub.org/public/igem/BBa_S04642/annotation2154769/range2154769/1
type: Range
persistent identity: range2154769
display id: range2154769
version: 1
top level: BBa_S04642
direction: direction/reverse
start: 1
end: 368
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2154770
display id: annotation2154770
version: 1
top level: BBa_S04642
location: https://synbiohub.org/public/igem/BBa_S04642/annotation2154770/range2154770/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04642/annotation2154770/range2154770/1
type: Range
persistent identity: range2154770
display id: range2154770
version: 1
top level: BBa_S04642
direction: direction/reverse
start: 369
end: 374
orientation: inline

BBa_J176019
type: SequenceAnnotation
persistent identity: annotation2154771
display id: annotation2154771
version: 1
top level: BBa_S04642
location: https://synbiohub.org/public/igem/BBa_S04642/annotation2154771/range2154771/1
component: BBa_J176019

https://synbiohub.org/public/igem/BBa_S04642/annotation2154771/range2154771/1
type: Range
persistent identity: range2154771
display id: range2154771
version: 1
top level: BBa_S04642
direction: direction/reverse
start: 375
end: 467
orientation: inline

BBa_S04704
type: ComponentDefinition
persistent identity: BBa S04704
display id: BBa_S04704
version: 1
was generated by: iGEM to SBOL conversion
description: J176012:J176018
created: 2011-10-06T11:00:00Z
modified: 2015-05-08T01:14:44Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J176042
component: BBa_J176041
component: BBa_J176008
component: BBa_J176016
component: BBa_J176022
component: BBa_J176012
component: BBa_J176018
sequence annotation: BBa_J176012
sequence annotation: BBa_J176041
sequence annotation: BBa_J176018
sequence annotation: BBa_J176022
sequence annotation: BBa_J176008
sequence annotation: BBa_J176016
sequence annotation: BBa_J176042
sequence: https://synbiohub.org/public/igem/BBa_S04704_sequence/1

BBa_J176042
type: Component
persistent identity: component2156825
display id: component2156825
version: 1
top level: BBa_S04704
definition: polyA
access: public

BBa_J176041
type: Component
persistent identity: component2156821
display id: component2156821
version: 1
top level: BBa_S04704
definition: scar
access: public

BBa_J176008
type: Component
persistent identity: component2156820
display id: component2156820
version: 1
top level: BBa_S04704
definition: NLS
access: public

BBa_J176041
type: Component
persistent identity: component2156823
display id: component2156823
version: 1
top level: BBa_S04704
definition: scar
access: public

BBa_J176016
type: Component
persistent identity: component2156822
display id: component2156822
version: 1
top level: BBa_S04704
definition: STOP
access: public

BBa_J176041
type: Component
persistent identity: component2156817
display id: component2156817
version: 1
top level: BBa_S04704
definition: scar
access: public

BBa_J176022
type: Component
persistent identity: component2156816
display id: component2156816
version: 1
top level: BBa_S04704
definition: AmCyan
access: public

BBa_J176041
type: Component
persistent identity: component2156819
display id: component2156819
version: 1
top level: BBa_S04704
definition: scar
access: public

BBa_J176022
type: Component
persistent identity: component2156818
display id: component2156818
version: 1
top level: BBa_S04704
definition: AmCyan
access: public

BBa_J176041
type: Component
persistent identity: component2156813
display id: component2156813
version: 1
top level: BBa_S04704
definition: scar
access: public

BBa_J176012
type: Component
persistent identity: component2156812
display id: component2156812
version: 1
top level: BBa_S04704
definition: K
access: public

BBa_J176041
type: Component
persistent identity: component2156815
display id: component2156815
version: 1
top level: BBa_S04704
definition: scar
access: public

BBa_J176018
type: Component
persistent identity: component2156814
display id: component2156814
version: 1
top level: BBa_S04704
definition: PEST
access: public

BBa_J176012
type: SequenceAnnotation
persistent identity: annotation2156812
display id: annotation2156812
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156812/range2156812/1
component: BBa_J176012

https://synbiohub.org/public/igem/BBa_S04704/annotation2156812/range2156812/1
type: Range
persistent identity: range2156812
display id: range2156812
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1
end: 18
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156813
display id: annotation2156813
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156813/range2156813/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04704/annotation2156813/range2156813/1
type: Range
persistent identity: range2156813
display id: range2156813
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 19
end: 24
orientation: inline

BBa_J176018
type: SequenceAnnotation
persistent identity: annotation2156814
display id: annotation2156814
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156814/range2156814/1
component: BBa_J176018

https://synbiohub.org/public/igem/BBa_S04704/annotation2156814/range2156814/1
type: Range
persistent identity: range2156814
display id: range2156814
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 25
end: 558
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156815
display id: annotation2156815
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156815/range2156815/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04704/annotation2156815/range2156815/1
type: Range
persistent identity: range2156815
display id: range2156815
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 559
end: 564
orientation: inline

BBa_J176022
type: SequenceAnnotation
persistent identity: annotation2156816
display id: annotation2156816
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156816/range2156816/1
component: BBa_J176022

https://synbiohub.org/public/igem/BBa_S04704/annotation2156816/range2156816/1
type: Range
persistent identity: range2156816
display id: range2156816
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 565
end: 1251
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156817
display id: annotation2156817
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156817/range2156817/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04704/annotation2156817/range2156817/1
type: Range
persistent identity: range2156817
display id: range2156817
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1252
end: 1257
orientation: inline

BBa_J176022
type: SequenceAnnotation
persistent identity: annotation2156818
display id: annotation2156818
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156818/range2156818/1
component: BBa_J176022

https://synbiohub.org/public/igem/BBa_S04704/annotation2156818/range2156818/1
type: Range
persistent identity: range2156818
display id: range2156818
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1258
end: 1944
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156819
display id: annotation2156819
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156819/range2156819/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04704/annotation2156819/range2156819/1
type: Range
persistent identity: range2156819
display id: range2156819
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1945
end: 1950
orientation: inline

BBa_J176008
type: SequenceAnnotation
persistent identity: annotation2156820
display id: annotation2156820
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156820/range2156820/1
component: BBa_J176008

https://synbiohub.org/public/igem/BBa_S04704/annotation2156820/range2156820/1
type: Range
persistent identity: range2156820
display id: range2156820
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1951
end: 1971
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156821
display id: annotation2156821
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156821/range2156821/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04704/annotation2156821/range2156821/1
type: Range
persistent identity: range2156821
display id: range2156821
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1972
end: 1977
orientation: inline

BBa_J176016
type: SequenceAnnotation
persistent identity: annotation2156822
display id: annotation2156822
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156822/range2156822/1
component: BBa_J176016

https://synbiohub.org/public/igem/BBa_S04704/annotation2156822/range2156822/1
type: Range
persistent identity: range2156822
display id: range2156822
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1978
end: 1990
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156823
display id: annotation2156823
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156823/range2156823/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04704/annotation2156823/range2156823/1
type: Range
persistent identity: range2156823
display id: range2156823
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1991
end: 1996
orientation: inline

BBa_J176042
type: SequenceAnnotation
persistent identity: annotation2156825
display id: annotation2156825
version: 1
top level: BBa_S04704
location: https://synbiohub.org/public/igem/BBa_S04704/annotation2156825/range2156825/1
component: BBa_J176042

https://synbiohub.org/public/igem/BBa_S04704/annotation2156825/range2156825/1
type: Range
persistent identity: range2156825
display id: range2156825
version: 1
top level: BBa_S04704
direction: direction/reverse
start: 1997
end: 2464
orientation: inline

Ubc
type: ComponentDefinition
persistent identity: BBa J176003
display id: BBa_J176003
version: 1
was generated by: iGEM to SBOL conversion
description: Ubc
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Ubc constitutive promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J176003_sequence/1

5xGal4
type: ComponentDefinition
persistent identity: BBa J176019
display id: BBa_J176019
version: 1
was generated by: iGEM to SBOL conversion
description: 5xGal4
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: target sequence of GAL4 DB; PCR cloned from pNEB
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Gal4
sequence: https://synbiohub.org/public/igem/BBa_J176019_sequence/1

Gal4
type: SequenceAnnotation
persistent identity: annotation2159559
display id: annotation2159559
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159559/range2159559/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J176019/annotation2159559/range2159559/1
type: Range
persistent identity: range2159559
display id: range2159559
version: 1
top level: 5xGal4
direction: direction/forward
start: 1
end: 17
orientation: inline

Gal4
type: SequenceAnnotation
persistent identity: annotation2159560
display id: annotation2159560
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159560/range2159560/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176019/annotation2159560/range2159560/1
type: Range
persistent identity: range2159560
display id: range2159560
version: 1
top level: 5xGal4
direction: direction/forward
start: 20
end: 36
orientation: inline

Gal4
type: SequenceAnnotation
persistent identity: annotation2159561
display id: annotation2159561
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159561/range2159561/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176019/annotation2159561/range2159561/1
type: Range
persistent identity: range2159561
display id: range2159561
version: 1
top level: 5xGal4
direction: direction/forward
start: 39
end: 55
orientation: inline

Gal4
type: SequenceAnnotation
persistent identity: annotation2159562
display id: annotation2159562
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159562/range2159562/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176019/annotation2159562/range2159562/1
type: Range
persistent identity: range2159562
display id: range2159562
version: 1
top level: 5xGal4
direction: direction/forward
start: 58
end: 74
orientation: inline

Gal4
type: SequenceAnnotation
persistent identity: annotation2159563
display id: annotation2159563
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159563/range2159563/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176019/annotation2159563/range2159563/1
type: Range
persistent identity: range2159563
display id: range2159563
version: 1
top level: 5xGal4
direction: direction/forward
start: 77
end: 93
orientation: inline

NLS
type: ComponentDefinition
persistent identity: BBa J176008
display id: BBa_J176008
version: 1
was generated by: iGEM to SBOL conversion
description: NLS
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: SV40 nuclear localization sequence
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176008_sequence/1

K
type: ComponentDefinition
persistent identity: BBa J176012
display id: BBa_J176012
version: 1
was generated by: iGEM to SBOL conversion
description: Kozak
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: yeast/ human Kozak plus start codon
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J176012_sequence/1

STOP
type: ComponentDefinition
persistent identity: BBa J176016
display id: BBa_J176016
version: 1
was generated by: iGEM to SBOL conversion
description: STOP
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: contains stop codons TAA and TGA in frame
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J176016_sequence/1

AmCyan
type: ComponentDefinition
persistent identity: BBa J176022
display id: BBa_J176022
version: 1
was generated by: iGEM to SBOL conversion
description: AmCyan
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: cyan fluorescent protein from PamCyan-1C1
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176022_sequence/1

polyA
type: ComponentDefinition
persistent identity: BBa J176042
display id: BBa_J176042
version: 1
was generated by: iGEM to SBOL conversion
description: polyA
created: 2011-10-06T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: Originally from the Silver Lab BioBrick collection. Biological source unknown. This part was created by David Drubin.
owned by: james
owned by: myers
mutable description: Polyadenylation signal for mammalian genes
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is the first version of polyA that we used to build many of our mammalian parts, until we switched to a streamlined, shorter polyA (polyA2, BBa_J176009).
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: questionable base call
sequence: https://synbiohub.org/public/igem/BBa_J176042_sequence/1

questionable base call
type: SequenceAnnotation
persistent identity: annotation2156673
display id: annotation2156673
version: 1
top level: polyA
location: https://synbiohub.org/public/igem/BBa_J176042/annotation2156673/range2156673/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176042/annotation2156673/range2156673/1
type: Range
persistent identity: range2156673
display id: range2156673
version: 1
top level: polyA
direction: direction/forward
start: 128
end: 128
orientation: inline

PEST
type: ComponentDefinition
persistent identity: BBa J176018
display id: BBa_J176018
version: 1
was generated by: iGEM to SBOL conversion
description: PEST
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: degradation tag
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_J176018_sequence/1

https://synbiohub.org/public/igem/BBa_J176050_sequence/1
type: Sequence
persistent identity: BBa J176050 sequence
display id: BBa_J176050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggagactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacccgccgccaccatggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttcccc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176041_sequence/1
type: Sequence
persistent identity: BBa J176041 sequence
display id: BBa_J176041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04642_sequence/1
type: Sequence
persistent identity: BBa S04642 sequence
display id: BBa_S04642_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggagactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04704_sequence/1
type: Sequence
persistent identity: BBa S04704 sequence
display id: BBa_S04704_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagaagac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176003_sequence/1
type: Sequence
persistent identity: BBa J176003 sequence
display id: BBa_J176003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaactggcctccgcgccgggcttttggcgcctcccgcgggcgcccccctcctcacggcgagcgctgccacgtcagacgaagggcgcagcgagcgtcctgatccttccgcccggacgctcaggacagcggcccgctgctcataagactcggccttagaaccccagtatcagcagaaggacattttaggacgggacttgggtgactctagggcactggttttctttccagagagcggaacaggcgaggaaaagtagtcccttctcggcgattctgcggagggatctccgtggggcggtgaacgccgatgattatataaggacgcgccgggtgtggcacagctagttactagacccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176008_sequence/1
type: Sequence
persistent identity: BBa J176008 sequence
display id: BBa_J176008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccaagaaaaagcgcaaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176012_sequence/1
type: Sequence
persistent identity: BBa J176012 sequence
display id: BBa_J176012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176016_sequence/1
type: Sequence
persistent identity: BBa J176016 sequence
display id: BBa_J176016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaaagctgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176019_sequence/1
type: Sequence
persistent identity: BBa J176019 sequence
display id: BBa_J176019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176022_sequence/1
type: Sequence
persistent identity: BBa J176022 sequence
display id: BBa_J176022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176042_sequence/1
type: Sequence
persistent identity: BBa J176042 sequence
display id: BBa_J176042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176018_sequence/1
type: Sequence
persistent identity: BBa J176018 sequence
display id: BBa_J176018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z