##gff-version 3
##sequence-region BBa_J176051 1 3085
BBa_J176051	.	engineered_region	1	615	.	+	0	ID=BBa_S04643;Name=BBa_S04643
BBa_J176051	.	sequence_feature	523	615	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04643
BBa_J176051	.	non_covalent_binding_site	542	558	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176051	.	non_covalent_binding_site	599	615	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176051	.	non_covalent_binding_site	580	596	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176051	.	non_covalent_binding_site	561	577	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176051	.	non_covalent_binding_site	523	539	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176051	.	sequence_feature	517	522	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04643
BBa_J176051	.	promoter	1	516	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04643
BBa_J176051	.	sequence_alteration	511	511	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176051	.	sequence_alteration	253	253	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176051	.	sequence_feature	616	621	.	+	0	ID=BBa_J176041;Name=BBa_J176041
BBa_J176051	.	engineered_region	622	3085	.	+	0	ID=BBa_S04704;Name=BBa_S04704
BBa_J176051	.	tag	2618	3085	.	+	0	ID=BBa_J176042;Name=BBa_J176042;Parent=BBa_S04704
BBa_J176051	.	sequence_feature	2745	2745	.	+	0	ID=annotation2156673;Name=questionable base call;Parent=BBa_J176042
BBa_J176051	.	polypeptide_domain	1879	2565	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04704
BBa_J176051	.	ribosome_entry_site	622	639	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04704
BBa_J176051	.	tag	646	1179	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_S04704
BBa_J176051	.	terminator	2599	2611	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04704
BBa_J176051	.	sequence_feature	1180	1185	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176051	.	sequence_feature	1873	1878	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176051	.	sequence_feature	2593	2598	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176051	.	sequence_feature	2566	2571	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176051	.	polypeptide_domain	1186	1872	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04704
BBa_J176051	.	sequence_feature	2612	2617	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176051	.	sequence_feature	640	645	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176051	.	tag	2572	2592	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04704
>BBa_J176051
ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctac
acaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcg
cgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatgga
agtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggc
caatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggc
tcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct
gttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtc
ctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacccgccgccac
catggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattca
aatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgata
acagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaa
cagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacg
ggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatc
ccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgg
gaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactaga
atggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactact
tcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacgg
cggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccacc
agcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcg
tggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccc
cgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatc
ttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaaga
ccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaa
cagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaag
ttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagg
gcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctc
cttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttc
aagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctggg
agatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgat
ggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgacc
gccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgacca
tgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccga
gcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagc
tgagactagaagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttat
ttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcatt
cattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctg
attatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctcca
tgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggc
ctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaa
gctt