LOCUS BBa_J176053 7859 bp DNA linear 22-Dec-2024 DEFINITION luc miR sensor 1 (R/Ubc-T/Ubc) ACCESSION BBa_J176053 VERSION BBa_J176053.1 FEATURES Location/Qualifiers promoter 1..368 /label=BBa_J176003 misc_feature 369..374 /label=BBa_J176041 misc_feature 375..391 /label=Gal4 misc_feature 394..410 /label=Gal4 misc_feature 413..429 /label=Gal4 misc_feature 432..448 /label=Gal4 misc_feature 451..467 /label=Gal4 misc_feature 375..467 /label=BBa_J176019 misc_feature 1..467 /label=BBa_S04642 misc_feature 468..473 /label=BBa_J176041 RBS 474..491 /label=BBa_J176012 misc_feature 492..497 /label=BBa_J176041 misc_feature 498..1031 /label=BBa_J176018 misc_feature 1032..1037 /label=BBa_J176041 misc_feature 1038..1724 /label=BBa_J176022 misc_feature 1725..1730 /label=BBa_J176041 misc_feature 1731..2417 /label=BBa_J176022 misc_feature 2418..2423 /label=BBa_J176041 misc_feature 2424..2444 /label=BBa_J176008 misc_feature 2445..2450 /label=BBa_J176041 terminator 2451..2463 /label=BBa_J176016 misc_feature 2464..2469 /label=BBa_J176041 misc_feature 2597..2597 /label=questionable base call misc_feature 2470..2937 /label=BBa_J176042 misc_feature 474..2937 /label=BBa_S04704 misc_feature 1..2937 /label=BBa_J176050 misc_feature 2938..2943 /label=BBa_J176041 misc_feature 2944..3283 /label=BBa_J176021 misc_feature 3284..3289 /label=BBa_J176041 misc_feature 3290..3629 /label=BBa_J176021 misc_feature 3630..3635 /label=BBa_J176041 promoter 3636..4003 /label=BBa_J176003 misc_feature 4004..4009 /label=BBa_J176041 RBS 4010..4027 /label=BBa_J176012 misc_feature 4028..4033 /label=BBa_J176041 misc_feature 4034..4492 /label=BBa_J176020 misc_feature 4493..4498 /label=BBa_J176041 misc_feature 4499..5032 /label=BBa_J176018 misc_feature 5033..5038 /label=BBa_J176041 misc_feature 5039..5743 /label=BBa_J176005 misc_feature 5744..5749 /label=BBa_J176041 misc_feature 5750..6454 /label=BBa_J176005 misc_feature 6455..6460 /label=BBa_J176041 misc_feature 6461..6481 /label=BBa_J176008 misc_feature 6482..6487 /label=BBa_J176041 terminator 6488..6500 /label=BBa_J176016 misc_feature 2944..6500 /label=BBa_S04699 misc_feature 6501..6506 /label=BBa_J176041 misc_feature 6534..6552 /label=repeat 1 misc_feature 6561..6579 /label=repeat 2 misc_feature 6588..6606 /label=repeat 3 misc_feature 6615..6633 /label=repeat 4 misc_feature 6641..6659 /label=repeat 5 misc_feature 6507..6693 /label=BBa_J176025 misc_feature 6694..6699 /label=BBa_J176041 misc_feature 6827..6827 /label=questionable base call misc_feature 6700..7167 /label=BBa_J176042 misc_feature 7168..7173 /label=BBa_J176041 misc_feature 7174..7513 /label=BBa_J176021 misc_feature 7514..7519 /label=BBa_J176041 misc_feature 7520..7859 /label=BBa_J176021 misc_feature 6507..7859 /label=BBa_S04691 misc_feature 2944..7859 /label=BBa_J176046 ORIGIN 1 aaactggcct ccgcgccggg cttttggcgc ctcccgcggg cgcccccctc ctcacggcga 61 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca 121 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca 181 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag 241 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac 301 gccgatgatt atataaggac gcgccgggtg tggcacagct agttactaga cccgccgcca 361 ccatggagac tagacggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac 421 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgact agacccgccg 481 ccaccatgga gactagagca tccaacttga acatttcgag aaagcttacc atatcaaccc 541 catcatgctc tttcgaaaat tcaaatagca catccattcc ttcgcccgct tcctcatctc 601 aaagccacac tccaatgaga aacatgagct cactctctga taacagcgtt ttcagccgga 661 atatggaaca atcatcacca atcactccaa gtatgtacca atttggtcag cagcagtcaa 721 acagtatatg tggtagcacc gttagtgtga atagtctggt gaatacaaat aacaaacaaa 781 ggatctacga acaaatcacg ggtcctaaca gcaataacgc aaccaatgat tatattgatt 841 tgctaaacct aaatgagtct aacaaggaaa accaaaatcc cgcaacggcg cattacctca 901 atgggggccc acccaagaca agcttcatta accatggaat gttcccctcg ccaactggga 961 ccataaatag cggtaaatct agcagtgcct catctttaat ttcttttggt atgggcaata 1021 cccaagtaat aactagaatg gccctgtcca acaagttcat cggcgacgac atgaagatga 1081 cctaccacat ggacggctgc gtgaacggcc actacttcac cgtgaagggc gagggcagcg 1141 gcaagcccta cgagggcacc cagacctcca ccttcaaggt gaccatggcc aacggcggcc 1201 ccctggcctt ctccttcgac atcctgtcca ccgtgttcat gtacggcaac cgctgcttca 1261 ccgcctaccc caccagcatg cccgactact tcaagcaggc cttccccgac ggcatgtcct 1321 acgagagaac cttcacctac gaggacggcg gcgtggccac cgccagctgg gagatcagcc 1381 tgaagggcaa ctgcttcgag cacaagtcca ccttccacgg cgtgaacttc cccgccgacg 1441 gccccgtgat ggccaagaag accaccggct gggacccctc cttcgagaag atgaccgtgt 1501 gcgacggcat cttgaagggc gacgtgaccg ccttcctgat gctgcaaggc ggcggcaact 1561 acagatgcca gttccacacc tcctacaaga ccaagaagcc cgtgaccatg ccccccaacc 1621 acgtggtgga gcaccgcatc gccagaaccg acctggacaa gggcggcaac agcgtgcagc 1681 tgaccgagca cgccgtggcc cacatcacct ccgtggtgcc cttcactaga atggccctgt 1741 ccaacaagtt catcggcgac gacatgaaga tgacctacca catggacggc tgcgtgaacg 1801 gccactactt caccgtgaag ggcgagggca gcggcaagcc ctacgagggc acccagacct 1861 ccaccttcaa ggtgaccatg gccaacggcg gccccctggc cttctccttc gacatcctgt 1921 ccaccgtgtt catgtacggc aaccgctgct tcaccgccta ccccaccagc atgcccgact 1981 acttcaagca ggccttcccc gacggcatgt cctacgagag aaccttcacc tacgaggacg 2041 gcggcgtggc caccgccagc tgggagatca gcctgaaggg caactgcttc gagcacaagt 2101 ccaccttcca cggcgtgaac ttccccgccg acggccccgt gatggccaag aagaccaccg 2161 gctgggaccc ctccttcgag aagatgaccg tgtgcgacgg catcttgaag ggcgacgtga 2221 ccgccttcct gatgctgcaa ggcggcggca actacagatg ccagttccac acctcctaca 2281 agaccaagaa gcccgtgacc atgcccccca accacgtggt ggagcaccgc atcgccagaa 2341 ccgacctgga caagggcggc aacagcgtgc agctgaccga gcacgccgtg gcccacatca 2401 cctccgtggt gcccttcact agacccaaga aaaagcgcaa ggtaactaga gcgtaaagct 2461 gagactagaa gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 2521 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 2581 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 2641 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta 2701 tgatcctgca agcctcgtcg tcctggccgg accacgctat ctgtgcaagg tccccggccc 2761 cggacgcgcg ctccatgagc agagcgcccg ccgccgaggc gaagactcgg gcggcgccct 2821 gcccgtccca ccaggtcaac aggcggtaac cggcctcttc atcgggaatg cgcgcgacct 2881 tcagcatcgc cggcatgtcc ccctggcgga cgggaagtat ccagctcgac caagcttact 2941 agattaatta actcgagtcc taaagaagtc ggcttctcca tcggatcctg atcaggggag 3001 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta 3061 gggggcagca gcgaccgccc ggggctccgc tccggtccgg cgctcccccc gcatcccgag 3121 ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg 3181 aacgcttctc gctgctcttt gagcctgcaa acacctgggg gatacgggga aaatgatcaa 3241 gatcttctac actgggacgc aaggattatc ctcgaggcgc gccactagat taattaactc 3301 gagtcctaaa gaagtcggct tctccatcgg atcctgatca ggggagctca cggggacagc 3361 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga 3421 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat cccgagccgg cagcgtgcgg 3481 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg 3541 ctctttgagc ctgcaaacac ctgggggata cggggaaaat gatcaagatc ttctacactg 3601 ggacgcaagg attatcctcg aggcgcgcca ctagaaaact ggcctccgcg ccgggctttt 3661 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg 3721 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga 3781 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc 3841 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg 3901 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc 3961 gggtgtggca cagctagtta ctagacccgc cgccaccatg gagactagac ccgccgccac 4021 catggagact agaatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact 4081 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg 4141 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga 4201 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga 4261 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt 4321 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac 4381 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag 4441 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa tttccgggga tcactagagc 4501 atccaacttg aacatttcga gaaagcttac catatcaacc ccatcatgct ctttcgaaaa 4561 ttcaaatagc acatccattc cttcgcccgc ttcctcatct caaagccaca ctccaatgag 4621 aaacatgagc tcactctctg ataacagcgt tttcagccgg aatatggaac aatcatcacc 4681 aatcactcca agtatgtacc aatttggtca gcagcagtca aacagtatat gtggtagcac 4741 cgttagtgtg aatagtctgg tgaatacaaa taacaaacaa aggatctacg aacaaatcac 4801 gggtcctaac agcaataacg caaccaatga ttatattgat ttgctaaacc taaatgagtc 4861 taacaaggaa aaccaaaatc ccgcaacggc gcattacctc aatgggggcc cacccaagac 4921 aagcttcatt aaccatggaa tgttcccctc gccaactggg accataaata gcggtaaatc 4981 tagcagtgcc tcatctttaa tttcttttgg tatgggcaat acccaagtaa taactagagt 5041 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca 5101 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc 5161 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc 5221 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc 5281 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat 5341 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga 5401 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca 5461 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct 5521 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt 5581 caagaccacc tacaaggcca agaagcccgt gcagctaccc ggcgcctaca acgtcaacat 5641 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc 5701 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagactagag tgagcaaggg 5761 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 5821 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 5881 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 5941 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 6001 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 6061 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 6121 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 6181 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 6241 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 6301 ctacaaggcc aagaagcccg tgcagctacc cggcgcctac aacgtcaaca tcaagttgga 6361 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 6421 ccactccacc ggcggcatgg acgagctgta caagactaga cccaagaaaa agcgcaaggt 6481 aactagagcg taaagctgag actagaatag ttgtttaaac gagctcgcta gcaaatcaag 6541 agtcctcata aaggaattaa aatcaagagt cctcataaag tctagcaaat caagagtcct 6601 cataaagcta gacaaatcaa gagtcctcat aaataattcc aatcaagagt cctcataaag 6661 tcgacctgca agcatgcaag ctgatccggc tgcactagaa gacatgataa gatacattga 6721 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg 6781 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa 6841 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta 6901 aaacctctac aaatgtggta tggctgatta tgatcctgca agcctcgtcg tcctggccgg 6961 accacgctat ctgtgcaagg tccccggccc cggacgcgcg ctccatgagc agagcgcccg 7021 ccgccgaggc gaagactcgg gcggcgccct gcccgtccca ccaggtcaac aggcggtaac 7081 cggcctcttc atcgggaatg cgcgcgacct tcagcatcgc cggcatgtcc ccctggcgga 7141 cgggaagtat ccagctcgac caagcttact agattaatta actcgagtcc taaagaagtc 7201 ggcttctcca tcggatcctg atcaggggag ctcacgggga cagccccccc ccaaagcccc 7261 cagggatgta attacgtccc tcccccgcta gggggcagca gcgaccgccc ggggctccgc 7321 tccggtccgg cgctcccccc gcatcccgag ccggcagcgt gcggggacag cccgggcacg 7381 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcaa 7441 acacctgggg gatacgggga aaatgatcaa gatcttctac actgggacgc aaggattatc 7501 ctcgaggcgc gccactagat taattaactc gagtcctaaa gaagtcggct tctccatcgg 7561 atcctgatca ggggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta 7621 cgtccctccc ccgctagggg gcagcagcga ccgcccgggg ctccgctccg gtccggcgct 7681 ccccccgcat cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg 7741 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcaaacac ctgggggata 7801 cggggaaaat gatcaagatc ttctacactg ggacgcaagg attatcctcg aggcgcgcc //