LOCUS BBa_J176054 8007 bp DNA linear 22-Dec-2024 DEFINITION luc miR sensor 2 (R/Ubc-T/HPK) ACCESSION BBa_J176054 VERSION BBa_J176054.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 1..615 /label=BBa_S04643 misc_feature 616..621 /label=BBa_J176041 RBS 622..639 /label=BBa_J176012 misc_feature 640..645 /label=BBa_J176041 misc_feature 646..1179 /label=BBa_J176018 misc_feature 1180..1185 /label=BBa_J176041 misc_feature 1186..1872 /label=BBa_J176022 misc_feature 1873..1878 /label=BBa_J176041 misc_feature 1879..2565 /label=BBa_J176022 misc_feature 2566..2571 /label=BBa_J176041 misc_feature 2572..2592 /label=BBa_J176008 misc_feature 2593..2598 /label=BBa_J176041 terminator 2599..2611 /label=BBa_J176016 misc_feature 2612..2617 /label=BBa_J176041 misc_feature 2745..2745 /label=questionable base call misc_feature 2618..3085 /label=BBa_J176042 misc_feature 622..3085 /label=BBa_S04704 misc_feature 1..3085 /label=BBa_J176051 misc_feature 3086..3091 /label=BBa_J176041 misc_feature 3092..3431 /label=BBa_J176021 misc_feature 3432..3437 /label=BBa_J176041 misc_feature 3438..3777 /label=BBa_J176021 misc_feature 3778..3783 /label=BBa_J176041 promoter 3784..4151 /label=BBa_J176003 misc_feature 4152..4157 /label=BBa_J176041 RBS 4158..4175 /label=BBa_J176012 misc_feature 4176..4181 /label=BBa_J176041 misc_feature 4182..4640 /label=BBa_J176020 misc_feature 4641..4646 /label=BBa_J176041 misc_feature 4647..5180 /label=BBa_J176018 misc_feature 5181..5186 /label=BBa_J176041 misc_feature 5187..5891 /label=BBa_J176005 misc_feature 5892..5897 /label=BBa_J176041 misc_feature 5898..6602 /label=BBa_J176005 misc_feature 6603..6608 /label=BBa_J176041 misc_feature 6609..6629 /label=BBa_J176008 misc_feature 6630..6635 /label=BBa_J176041 terminator 6636..6648 /label=BBa_J176016 misc_feature 3092..6648 /label=BBa_S04699 misc_feature 6649..6654 /label=BBa_J176041 misc_feature 6682..6700 /label=repeat 1 misc_feature 6709..6727 /label=repeat 2 misc_feature 6736..6754 /label=repeat 3 misc_feature 6763..6781 /label=repeat 4 misc_feature 6789..6807 /label=repeat 5 misc_feature 6655..6841 /label=BBa_J176025 misc_feature 6842..6847 /label=BBa_J176041 misc_feature 6975..6975 /label=questionable base call misc_feature 6848..7315 /label=BBa_J176042 misc_feature 7316..7321 /label=BBa_J176041 misc_feature 7322..7661 /label=BBa_J176021 misc_feature 7662..7667 /label=BBa_J176041 misc_feature 7668..8007 /label=BBa_J176021 misc_feature 6655..8007 /label=BBa_S04691 misc_feature 3092..8007 /label=BBa_J176046 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acccgccgcc accatggaga ctagagcatc caacttgaac 661 atttcgagaa agcttaccat atcaacccca tcatgctctt tcgaaaattc aaatagcaca 721 tccattcctt cgcccgcttc ctcatctcaa agccacactc caatgagaaa catgagctca 781 ctctctgata acagcgtttt cagccggaat atggaacaat catcaccaat cactccaagt 841 atgtaccaat ttggtcagca gcagtcaaac agtatatgtg gtagcaccgt tagtgtgaat 901 agtctggtga atacaaataa caaacaaagg atctacgaac aaatcacggg tcctaacagc 961 aataacgcaa ccaatgatta tattgatttg ctaaacctaa atgagtctaa caaggaaaac 1021 caaaatcccg caacggcgca ttacctcaat gggggcccac ccaagacaag cttcattaac 1081 catggaatgt tcccctcgcc aactgggacc ataaatagcg gtaaatctag cagtgcctca 1141 tctttaattt cttttggtat gggcaatacc caagtaataa ctagaatggc cctgtccaac 1201 aagttcatcg gcgacgacat gaagatgacc taccacatgg acggctgcgt gaacggccac 1261 tacttcaccg tgaagggcga gggcagcggc aagccctacg agggcaccca gacctccacc 1321 ttcaaggtga ccatggccaa cggcggcccc ctggccttct ccttcgacat cctgtccacc 1381 gtgttcatgt acggcaaccg ctgcttcacc gcctacccca ccagcatgcc cgactacttc 1441 aagcaggcct tccccgacgg catgtcctac gagagaacct tcacctacga ggacggcggc 1501 gtggccaccg ccagctggga gatcagcctg aagggcaact gcttcgagca caagtccacc 1561 ttccacggcg tgaacttccc cgccgacggc cccgtgatgg ccaagaagac caccggctgg 1621 gacccctcct tcgagaagat gaccgtgtgc gacggcatct tgaagggcga cgtgaccgcc 1681 ttcctgatgc tgcaaggcgg cggcaactac agatgccagt tccacacctc ctacaagacc 1741 aagaagcccg tgaccatgcc ccccaaccac gtggtggagc accgcatcgc cagaaccgac 1801 ctggacaagg gcggcaacag cgtgcagctg accgagcacg ccgtggccca catcacctcc 1861 gtggtgccct tcactagaat ggccctgtcc aacaagttca tcggcgacga catgaagatg 1921 acctaccaca tggacggctg cgtgaacggc cactacttca ccgtgaaggg cgagggcagc 1981 ggcaagccct acgagggcac ccagacctcc accttcaagg tgaccatggc caacggcggc 2041 cccctggcct tctccttcga catcctgtcc accgtgttca tgtacggcaa ccgctgcttc 2101 accgcctacc ccaccagcat gcccgactac ttcaagcagg ccttccccga cggcatgtcc 2161 tacgagagaa ccttcaccta cgaggacggc ggcgtggcca ccgccagctg ggagatcagc 2221 ctgaagggca actgcttcga gcacaagtcc accttccacg gcgtgaactt ccccgccgac 2281 ggccccgtga tggccaagaa gaccaccggc tgggacccct ccttcgagaa gatgaccgtg 2341 tgcgacggca tcttgaaggg cgacgtgacc gccttcctga tgctgcaagg cggcggcaac 2401 tacagatgcc agttccacac ctcctacaag accaagaagc ccgtgaccat gccccccaac 2461 cacgtggtgg agcaccgcat cgccagaacc gacctggaca agggcggcaa cagcgtgcag 2521 ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc ccttcactag acccaagaaa 2581 aagcgcaagg taactagagc gtaaagctga gactagaaga catgataaga tacattgatg 2641 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg 2701 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt 2761 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa 2821 acctctacaa atgtggtatg gctgattatg atcctgcaag cctcgtcgtc ctggccggac 2881 cacgctatct gtgcaaggtc cccggccccg gacgcgcgct ccatgagcag agcgcccgcc 2941 gccgaggcga agactcgggc ggcgccctgc ccgtcccacc aggtcaacag gcggtaaccg 3001 gcctcttcat cgggaatgcg cgcgaccttc agcatcgccg gcatgtcccc ctggcggacg 3061 ggaagtatcc agctcgacca agcttactag attaattaac tcgagtccta aagaagtcgg 3121 cttctccatc ggatcctgat caggggagct cacggggaca gccccccccc aaagccccca 3181 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gaccgcccgg ggctccgctc 3241 cggtccggcg ctccccccgc atcccgagcc ggcagcgtgc ggggacagcc cgggcacggg 3301 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcaaac 3361 acctggggga tacggggaaa atgatcaaga tcttctacac tgggacgcaa ggattatcct 3421 cgaggcgcgc cactagatta attaactcga gtcctaaaga agtcggcttc tccatcggat 3481 cctgatcagg ggagctcacg gggacagccc ccccccaaag cccccaggga tgtaattacg 3541 tccctccccc gctagggggc agcagcgacc gcccggggct ccgctccggt ccggcgctcc 3601 ccccgcatcc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg 3661 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcaaacacct gggggatacg 3721 gggaaaatga tcaagatctt ctacactggg acgcaaggat tatcctcgag gcgcgccact 3781 agaaaactgg cctccgcgcc gggcttttgg cgcctcccgc gggcgccccc ctcctcacgg 3841 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc 3901 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg 3961 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa 4021 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg 4081 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttact agacccgccg 4141 ccaccatgga gactagaccc gccgccacca tggagactag aatgaagcta ctgtcttcta 4201 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 4261 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 4321 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 4381 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 4441 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 4501 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 4561 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 4621 cgccggaatt tccggggatc actagagcat ccaacttgaa catttcgaga aagcttacca 4681 tatcaacccc atcatgctct ttcgaaaatt caaatagcac atccattcct tcgcccgctt 4741 cctcatctca aagccacact ccaatgagaa acatgagctc actctctgat aacagcgttt 4801 tcagccggaa tatggaacaa tcatcaccaa tcactccaag tatgtaccaa tttggtcagc 4861 agcagtcaaa cagtatatgt ggtagcaccg ttagtgtgaa tagtctggtg aatacaaata 4921 acaaacaaag gatctacgaa caaatcacgg gtcctaacag caataacgca accaatgatt 4981 atattgattt gctaaaccta aatgagtcta acaaggaaaa ccaaaatccc gcaacggcgc 5041 attacctcaa tgggggccca cccaagacaa gcttcattaa ccatggaatg ttcccctcgc 5101 caactgggac cataaatagc ggtaaatcta gcagtgcctc atctttaatt tcttttggta 5161 tgggcaatac ccaagtaata actagagtga gcaagggcga ggaggataac atggccatca 5221 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 5281 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 5341 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 5401 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 5461 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 5521 cccaggactc ctccttgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 5581 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 5641 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 5701 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 5761 agctacccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 5821 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 5881 agctgtacaa gactagagtg agcaagggcg aggaggataa catggccatc atcaaggagt 5941 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 6001 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 6061 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 6121 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 6181 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 6241 cctccttgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 6301 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 6361 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 6421 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctacccg 6481 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 6541 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 6601 agactagacc caagaaaaag cgcaaggtaa ctagagcgta aagctgagac tagaatagtt 6661 gtttaaacga gctcgctagc aaatcaagag tcctcataaa ggaattaaaa tcaagagtcc 6721 tcataaagtc tagcaaatca agagtcctca taaagctaga caaatcaaga gtcctcataa 6781 ataattccaa tcaagagtcc tcataaagtc gacctgcaag catgcaagct gatccggctg 6841 cactagaaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt 6901 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa 6961 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg 7021 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg 7081 atcctgcaag cctcgtcgtc ctggccggac cacgctatct gtgcaaggtc cccggccccg 7141 gacgcgcgct ccatgagcag agcgcccgcc gccgaggcga agactcgggc ggcgccctgc 7201 ccgtcccacc aggtcaacag gcggtaaccg gcctcttcat cgggaatgcg cgcgaccttc 7261 agcatcgccg gcatgtcccc ctggcggacg ggaagtatcc agctcgacca agcttactag 7321 attaattaac tcgagtccta aagaagtcgg cttctccatc ggatcctgat caggggagct 7381 cacggggaca gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg 7441 gggcagcagc gaccgcccgg ggctccgctc cggtccggcg ctccccccgc atcccgagcc 7501 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa 7561 cgcttctcgc tgctctttga gcctgcaaac acctggggga tacggggaaa atgatcaaga 7621 tcttctacac tgggacgcaa ggattatcct cgaggcgcgc cactagatta attaactcga 7681 gtcctaaaga agtcggcttc tccatcggat cctgatcagg ggagctcacg gggacagccc 7741 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgacc 7801 gcccggggct ccgctccggt ccggcgctcc ccccgcatcc cgagccggca gcgtgcgggg 7861 acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct 7921 ctttgagcct gcaaacacct gggggatacg gggaaaatga tcaagatctt ctacactggg 7981 acgcaaggat tatcctcgag gcgcgcc //