LOCUS BBa_J176057 8155 bp DNA linear 22-Nov-2024 DEFINITION luc miR sensor 5 (R/HPK-T/HPK) ACCESSION BBa_J176057 VERSION BBa_J176057.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 1..615 /label=BBa_S04643 misc_feature 616..621 /label=BBa_J176041 RBS 622..639 /label=BBa_J176012 misc_feature 640..645 /label=BBa_J176041 misc_feature 646..1179 /label=BBa_J176018 misc_feature 1180..1185 /label=BBa_J176041 misc_feature 1186..1872 /label=BBa_J176022 misc_feature 1873..1878 /label=BBa_J176041 misc_feature 1879..2565 /label=BBa_J176022 misc_feature 2566..2571 /label=BBa_J176041 misc_feature 2572..2592 /label=BBa_J176008 misc_feature 2593..2598 /label=BBa_J176041 terminator 2599..2611 /label=BBa_J176016 misc_feature 2612..2617 /label=BBa_J176041 misc_feature 2745..2745 /label=questionable base call misc_feature 2618..3085 /label=BBa_J176042 misc_feature 622..3085 /label=BBa_S04704 misc_feature 1..3085 /label=BBa_J176051 misc_feature 3086..3091 /label=BBa_J176041 misc_feature 3092..3431 /label=BBa_J176021 misc_feature 3432..3437 /label=BBa_J176041 misc_feature 3438..3777 /label=BBa_J176021 misc_feature 3778..3783 /label=BBa_J176041 misc_feature 4036..4036 /label=mutated SpeI misc_feature 4294..4294 /label=mutated PstI promoter 3784..4299 /label=BBa_J176028 misc_feature 4300..4305 /label=BBa_J176041 RBS 4306..4323 /label=BBa_J176012 misc_feature 4324..4329 /label=BBa_J176041 misc_feature 4330..4788 /label=BBa_J176020 misc_feature 4789..4794 /label=BBa_J176041 misc_feature 4795..5328 /label=BBa_J176018 misc_feature 5329..5334 /label=BBa_J176041 misc_feature 5335..6039 /label=BBa_J176005 misc_feature 6040..6045 /label=BBa_J176041 misc_feature 6046..6750 /label=BBa_J176005 misc_feature 6751..6756 /label=BBa_J176041 misc_feature 6757..6777 /label=BBa_J176008 misc_feature 6778..6783 /label=BBa_J176041 terminator 6784..6796 /label=BBa_J176016 misc_feature 3092..6796 /label=BBa_S04700 misc_feature 6797..6802 /label=BBa_J176041 misc_feature 6830..6848 /label=repeat 1 misc_feature 6857..6875 /label=repeat 2 misc_feature 6884..6902 /label=repeat 3 misc_feature 6911..6929 /label=repeat 4 misc_feature 6937..6955 /label=repeat 5 misc_feature 6803..6989 /label=BBa_J176025 misc_feature 6990..6995 /label=BBa_J176041 misc_feature 7123..7123 /label=questionable base call misc_feature 6996..7463 /label=BBa_J176042 misc_feature 7464..7469 /label=BBa_J176041 misc_feature 7470..7809 /label=BBa_J176021 misc_feature 7810..7815 /label=BBa_J176041 misc_feature 7816..8155 /label=BBa_J176021 misc_feature 6803..8155 /label=BBa_S04691 misc_feature 3092..8155 /label=BBa_J176047 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acccgccgcc accatggaga ctagagcatc caacttgaac 661 atttcgagaa agcttaccat atcaacccca tcatgctctt tcgaaaattc aaatagcaca 721 tccattcctt cgcccgcttc ctcatctcaa agccacactc caatgagaaa catgagctca 781 ctctctgata acagcgtttt cagccggaat atggaacaat catcaccaat cactccaagt 841 atgtaccaat ttggtcagca gcagtcaaac agtatatgtg gtagcaccgt tagtgtgaat 901 agtctggtga atacaaataa caaacaaagg atctacgaac aaatcacggg tcctaacagc 961 aataacgcaa ccaatgatta tattgatttg ctaaacctaa atgagtctaa caaggaaaac 1021 caaaatcccg caacggcgca ttacctcaat gggggcccac ccaagacaag cttcattaac 1081 catggaatgt tcccctcgcc aactgggacc ataaatagcg gtaaatctag cagtgcctca 1141 tctttaattt cttttggtat gggcaatacc caagtaataa ctagaatggc cctgtccaac 1201 aagttcatcg gcgacgacat gaagatgacc taccacatgg acggctgcgt gaacggccac 1261 tacttcaccg tgaagggcga gggcagcggc aagccctacg agggcaccca gacctccacc 1321 ttcaaggtga ccatggccaa cggcggcccc ctggccttct ccttcgacat cctgtccacc 1381 gtgttcatgt acggcaaccg ctgcttcacc gcctacccca ccagcatgcc cgactacttc 1441 aagcaggcct tccccgacgg catgtcctac gagagaacct tcacctacga ggacggcggc 1501 gtggccaccg ccagctggga gatcagcctg aagggcaact gcttcgagca caagtccacc 1561 ttccacggcg tgaacttccc cgccgacggc cccgtgatgg ccaagaagac caccggctgg 1621 gacccctcct tcgagaagat gaccgtgtgc gacggcatct tgaagggcga cgtgaccgcc 1681 ttcctgatgc tgcaaggcgg cggcaactac agatgccagt tccacacctc ctacaagacc 1741 aagaagcccg tgaccatgcc ccccaaccac gtggtggagc accgcatcgc cagaaccgac 1801 ctggacaagg gcggcaacag cgtgcagctg accgagcacg ccgtggccca catcacctcc 1861 gtggtgccct tcactagaat ggccctgtcc aacaagttca tcggcgacga catgaagatg 1921 acctaccaca tggacggctg cgtgaacggc cactacttca ccgtgaaggg cgagggcagc 1981 ggcaagccct acgagggcac ccagacctcc accttcaagg tgaccatggc caacggcggc 2041 cccctggcct tctccttcga catcctgtcc accgtgttca tgtacggcaa ccgctgcttc 2101 accgcctacc ccaccagcat gcccgactac ttcaagcagg ccttccccga cggcatgtcc 2161 tacgagagaa ccttcaccta cgaggacggc ggcgtggcca ccgccagctg ggagatcagc 2221 ctgaagggca actgcttcga gcacaagtcc accttccacg gcgtgaactt ccccgccgac 2281 ggccccgtga tggccaagaa gaccaccggc tgggacccct ccttcgagaa gatgaccgtg 2341 tgcgacggca tcttgaaggg cgacgtgacc gccttcctga tgctgcaagg cggcggcaac 2401 tacagatgcc agttccacac ctcctacaag accaagaagc ccgtgaccat gccccccaac 2461 cacgtggtgg agcaccgcat cgccagaacc gacctggaca agggcggcaa cagcgtgcag 2521 ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc ccttcactag acccaagaaa 2581 aagcgcaagg taactagagc gtaaagctga gactagaaga catgataaga tacattgatg 2641 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg 2701 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt 2761 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa 2821 acctctacaa atgtggtatg gctgattatg atcctgcaag cctcgtcgtc ctggccggac 2881 cacgctatct gtgcaaggtc cccggccccg gacgcgcgct ccatgagcag agcgcccgcc 2941 gccgaggcga agactcgggc ggcgccctgc ccgtcccacc aggtcaacag gcggtaaccg 3001 gcctcttcat cgggaatgcg cgcgaccttc agcatcgccg gcatgtcccc ctggcggacg 3061 ggaagtatcc agctcgacca agcttactag attaattaac tcgagtccta aagaagtcgg 3121 cttctccatc ggatcctgat caggggagct cacggggaca gccccccccc aaagccccca 3181 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gaccgcccgg ggctccgctc 3241 cggtccggcg ctccccccgc atcccgagcc ggcagcgtgc ggggacagcc cgggcacggg 3301 gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcaaac 3361 acctggggga tacggggaaa atgatcaaga tcttctacac tgggacgcaa ggattatcct 3421 cgaggcgcgc cactagatta attaactcga gtcctaaaga agtcggcttc tccatcggat 3481 cctgatcagg ggagctcacg gggacagccc ccccccaaag cccccaggga tgtaattacg 3541 tccctccccc gctagggggc agcagcgacc gcccggggct ccgctccggt ccggcgctcc 3601 ccccgcatcc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg 3661 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcaaacacct gggggatacg 3721 gggaaaatga tcaagatctt ctacactggg acgcaaggat tatcctcgag gcgcgccact 3781 agactaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 3841 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 3901 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 3961 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 4021 agtagcacgt ctcacaagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 4081 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 4141 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 4201 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 4261 ctcctcttcc tcatctccgg gcctttcgac ctggagccca ctagacccgc cgccaccatg 4321 gagactagaa tgaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa 4381 aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag 4441 tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct gacagaagtg 4501 gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg agaagacctt 4561 gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac aggattattt 4621 gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt ggagactgat 4681 atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga agagagtagt 4741 aacaaaggtc aaagacagtt gactgtatcg ccggaatttc cggggatcac tagagcatcc 4801 aacttgaaca tttcgagaaa gcttaccata tcaaccccat catgctcttt cgaaaattca 4861 aatagcacat ccattccttc gcccgcttcc tcatctcaaa gccacactcc aatgagaaac 4921 atgagctcac tctctgataa cagcgttttc agccggaata tggaacaatc atcaccaatc 4981 actccaagta tgtaccaatt tggtcagcag cagtcaaaca gtatatgtgg tagcaccgtt 5041 agtgtgaata gtctggtgaa tacaaataac aaacaaagga tctacgaaca aatcacgggt 5101 cctaacagca ataacgcaac caatgattat attgatttgc taaacctaaa tgagtctaac 5161 aaggaaaacc aaaatcccgc aacggcgcat tacctcaatg ggggcccacc caagacaagc 5221 ttcattaacc atggaatgtt cccctcgcca actgggacca taaatagcgg taaatctagc 5281 agtgcctcat ctttaatttc ttttggtatg ggcaataccc aagtaataac tagagtgagc 5341 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg 5401 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac 5461 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg 5521 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac 5581 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac 5641 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccttgcagga cggcgagttc 5701 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag 5761 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag 5821 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 5881 accacctaca aggccaagaa gcccgtgcag ctacccggcg cctacaacgt caacatcaag 5941 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 6001 ggccgccact ccaccggcgg catggacgag ctgtacaaga ctagagtgag caagggcgag 6061 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc 6121 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc 6181 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg 6241 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac 6301 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac 6361 ggcggcgtgg tgaccgtgac ccaggactcc tccttgcagg acggcgagtt catctacaag 6421 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg 6481 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc 6541 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac 6601 aaggccaaga agcccgtgca gctacccggc gcctacaacg tcaacatcaa gttggacatc 6661 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac 6721 tccaccggcg gcatggacga gctgtacaag actagaccca agaaaaagcg caaggtaact 6781 agagcgtaaa gctgagacta gaatagttgt ttaaacgagc tcgctagcaa atcaagagtc 6841 ctcataaagg aattaaaatc aagagtcctc ataaagtcta gcaaatcaag agtcctcata 6901 aagctagaca aatcaagagt cctcataaat aattccaatc aagagtcctc ataaagtcga 6961 cctgcaagca tgcaagctga tccggctgca ctagaagaca tgataagata cattgatgag 7021 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 7081 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 7141 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac 7201 ctctacaaat gtggtatggc tgattatgat cctgcaagcc tcgtcgtcct ggccggacca 7261 cgctatctgt gcaaggtccc cggccccgga cgcgcgctcc atgagcagag cgcccgccgc 7321 cgaggcgaag actcgggcgg cgccctgccc gtcccaccag gtcaacaggc ggtaaccggc 7381 ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct ggcggacggg 7441 aagtatccag ctcgaccaag cttactagat taattaactc gagtcctaaa gaagtcggct 7501 tctccatcgg atcctgatca ggggagctca cggggacagc ccccccccaa agcccccagg 7561 gatgtaatta cgtccctccc ccgctagggg gcagcagcga ccgcccgggg ctccgctccg 7621 gtccggcgct ccccccgcat cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga 7681 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcaaacac 7741 ctgggggata cggggaaaat gatcaagatc ttctacactg ggacgcaagg attatcctcg 7801 aggcgcgcca ctagattaat taactcgagt cctaaagaag tcggcttctc catcggatcc 7861 tgatcagggg agctcacggg gacagccccc ccccaaagcc cccagggatg taattacgtc 7921 cctcccccgc tagggggcag cagcgaccgc ccggggctcc gctccggtcc ggcgctcccc 7981 ccgcatcccg agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat 8041 cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc aaacacctgg gggatacggg 8101 gaaaatgatc aagatcttct acactgggac gcaaggatta tcctcgaggc gcgcc //