This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard).
Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard).
The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts.
Tetrameric VP16 transc. activator domain
complete tet./ dox. inducible promoter from Invitrogen vector pcDNA/FRT/TO, T-Rex system
NLS followed by myc epitope and STOP (MB_0018)
Danio rerio (zebrafish) CBX2 amino acids 2-64. The human codon-optimized coding sequence was synthesized by Genscript.
Amino acids 2-64 from the zebrafish CBX2 protein. This is the conserved Polycomb chromodomain predicted to bind trimethylated histone H3 lysine 27 in chromatin.
Codons were optimized for expression in cultured human cells.
Conversion of the iGEM parts registry to SBOL2.1