LOCUS       BBa_J176071             2452 bp    DNA     linear       22-Dec-2024
DEFINITION  EED expression casette 1
ACCESSION   BBa_J176071
VERSION     BBa_J176071.1  
FEATURES             Location/Qualifiers
     misc_feature    253..253
                     /label=mutated SpeI
     misc_feature    511..511
                     /label=mutated PstI
     promoter        1..516
                     /label=BBa_J176028
     misc_feature    517..522
                     /label=BBa_J176041
     RBS             523..540
                     /label=BBa_J176012
     misc_feature    1..540
                     /label=BBa_S04645
     misc_feature    541..546
                     /label=BBa_J176041
     misc_feature    547..1005
                     /label=BBa_J176020
     misc_feature    1006..1011
                     /label=BBa_J176041
     misc_feature    1012..2412
                     /label=BBa_J176023
     misc_feature    547..2412
                     /label=BBa_S04680
     misc_feature    2413..2418
                     /label=BBa_J176041
     misc_feature    2419..2452
                     /label=BBa_J176024
ORIGIN
        1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
       61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
      481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacccgccgc caccatggag
      541 actagaatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag
      601 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
      661 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa
      721 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac
      781 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta
      841 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg
      901 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac
      961 aaaggtcaaa gacagttgac tgtatcgccg gaatttccgg ggatcactag aatgtccgag
     1021 agggaagtgt cgactgcgcc ggcgggaaca gacatgcctg cggccaagaa gcagaagctg
     1081 agcagtgacg agaacagcaa tccagacctc tctggagacg agaatgatga cgctgtcagt
     1141 atagaaagtg gtacaaacac tgaacgccct gatacaccta caaacacgcc aaatgcacct
     1201 ggaaggaaaa gttggggaaa gggaaaatgg aagtcaaaga aatgcaaata ttctttcaaa
     1261 tgtgtaaata gtctcaagga agatcataac caaccattgt ttggagttca gtttaactgg
     1321 cacagtaaag aaggagatcc attagtgttt gcaactgtag gaagcaacag agttaccttg
     1381 tatgaatgtc attcacaagg agaaatccgg ttgttgcaat cttacgtgga tgctgatgct
     1441 gatgaaaact tttacacttg tgcatggacc tatgatagca atacgagcca tcctctgctg
     1501 gctgtagctg gatcaagagg cataattagg ataataaatc ctataacaat gcagtgtata
     1561 aagcactatg ttggccatgg aaatgctatc aatgagctga aattccatcc aagagatcca
     1621 aatcttctcc tgtcagtaag taaagatcat gctttacgat tatggaatat ccagacggac
     1681 actctggtgg caatatttgg aggcgtagaa gggcacagag atgaagttct aagtgctgat
     1741 tatgatcttt tgggtgaaaa aataatgtcc tgtggtatgg atcattctct taaactttgg
     1801 aggatcaatt caaagagaat gatgaatgca attaaggaat cttatgatta taatccaaat
     1861 aaaactaaca ggccatttat ttctcagaaa atccattttc ctgatttttc taccagagac
     1921 atacatagga attatgttga ttgtgtgcga tggttaggcg atttgatact ttctaagagt
     1981 ggccgtgcca ttttacattc ccaccagcaa tgtatgagag atccagtgtc tccgaatctt
     2041 cgccagcatt tgtcttgtga aaatgccatt gtgtgctgga aacctggcaa gatggaagat
     2101 gatatagata aaattaaacc cagtgaatct aatgtgacta ttcttgggcg atttgattac
     2161 agccagtgtg acatttggta catgaggttt tctatggatt tctggcaaaa gatgcttgca
     2221 ttgggcaatc aagttggcaa actttatgtt tgggatttag aagtagaaga tcctcataaa
     2281 gccaaatgta caacactgac tcatcataaa tgtggtgctg ctattcgaca aaccagtttt
     2341 agcagggata gcagcattct tatagctgtt tgtgatgatg ccagtatttg gcgctgggat
     2401 cgacttcgat tgactagacc caagaaaaag cgcaaggtag cgtaaagctg ag
//