##gff-version 3
##sequence-region BBa_J176071 1 2452
BBa_J176071	.	engineered_region	547	2412	.	+	0	ID=BBa_S04680;Name=BBa_S04680
BBa_J176071	.	polypeptide_domain	547	1005	.	+	0	ID=BBa_J176020;Name=BBa_J176020;Parent=BBa_S04680
BBa_J176071	.	sequence_feature	1012	2412	.	+	0	ID=BBa_J176023;Name=BBa_J176023;Parent=BBa_S04680
BBa_J176071	.	sequence_feature	1006	1011	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04680
BBa_J176071	.	sequence_feature	2413	2418	.	+	0	ID=BBa_J176041;Name=BBa_J176041
BBa_J176071	.	sequence_feature	2419	2452	.	+	0	ID=BBa_J176024;Name=BBa_J176024
BBa_J176071	.	sequence_feature	541	546	.	+	0	ID=BBa_J176041;Name=BBa_J176041
BBa_J176071	.	engineered_region	1	540	.	+	0	ID=BBa_S04645;Name=BBa_S04645
BBa_J176071	.	ribosome_entry_site	523	540	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04645
BBa_J176071	.	sequence_feature	517	522	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04645
BBa_J176071	.	promoter	1	516	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04645
BBa_J176071	.	sequence_alteration	511	511	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176071	.	sequence_alteration	253	253	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
>BBa_J176071
ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctac
acaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcg
cgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatgga
agtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggc
caatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggc
tcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct
gttctcctcttcctcatctccgggcctttcgacctggagcccactagacccgccgccaccatggagactagaatgaagc
tactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgc
caagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgaca
gaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttga
aaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcac
agatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaa
gagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagaatgtccgagagggaag
tgtcgactgcgccggcgggaacagacatgcctgcggccaagaagcagaagctgagcagtgacgagaacagcaatccaga
cctctctggagacgagaatgatgacgctgtcagtatagaaagtggtacaaacactgaacgccctgatacacctacaaac
acgccaaatgcacctggaaggaaaagttggggaaagggaaaatggaagtcaaagaaatgcaaatattctttcaaatgtg
taaatagtctcaaggaagatcataaccaaccattgtttggagttcagtttaactggcacagtaaagaaggagatccatt
agtgtttgcaactgtaggaagcaacagagttaccttgtatgaatgtcattcacaaggagaaatccggttgttgcaatct
tacgtggatgctgatgctgatgaaaacttttacacttgtgcatggacctatgatagcaatacgagccatcctctgctgg
ctgtagctggatcaagaggcataattaggataataaatcctataacaatgcagtgtataaagcactatgttggccatgg
aaatgctatcaatgagctgaaattccatccaagagatccaaatcttctcctgtcagtaagtaaagatcatgctttacga
ttatggaatatccagacggacactctggtggcaatatttggaggcgtagaagggcacagagatgaagttctaagtgctg
attatgatcttttgggtgaaaaaataatgtcctgtggtatggatcattctcttaaactttggaggatcaattcaaagag
aatgatgaatgcaattaaggaatcttatgattataatccaaataaaactaacaggccatttatttctcagaaaatccat
tttcctgatttttctaccagagacatacataggaattatgttgattgtgtgcgatggttaggcgatttgatactttcta
agagtggccgtgccattttacattcccaccagcaatgtatgagagatccagtgtctccgaatcttcgccagcatttgtc
ttgtgaaaatgccattgtgtgctggaaacctggcaagatggaagatgatatagataaaattaaacccagtgaatctaat
gtgactattcttgggcgatttgattacagccagtgtgacatttggtacatgaggttttctatggatttctggcaaaaga
tgcttgcattgggcaatcaagttggcaaactttatgtttgggatttagaagtagaagatcctcataaagccaaatgtac
aacactgactcatcataaatgtggtgctgctattcgacaaaccagttttagcagggatagcagcattcttatagctgtt
tgtgatgatgccagtatttggcgctgggatcgacttcgattgactagacccaagaaaaagcgcaaggtagcgtaaagct
gag