BBa_J176072
type: ComponentDefinition
persistent identity: BBa J176072
display id: BBa_J176072
version: 1
was generated by: iGEM to SBOL conversion
description: EED expression casette 2
created: 2011-10-13T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: EED expression casette 2
discontinued: false
dominant: false
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_S04679
component: BBa_J176041
component: BBa_J176071
sequence annotation: BBa_J176071
sequence annotation: BBa_J176041
sequence annotation: BBa_S04679
sequence: https://synbiohub.org/public/igem/BBa_J176072_sequence/1

BBa_S04679
type: Component
persistent identity: component2158258
display id: component2158258
version: 1
top level: BBa_J176072
definition: BBa_S04679
access: public

BBa_J176041
type: Component
persistent identity: component2158251
display id: component2158251
version: 1
top level: BBa_J176072
definition: scar
access: public

BBa_J176071
type: Component
persistent identity: component2158250
display id: component2158250
version: 1
top level: BBa_J176072
definition: BBa_J176071
access: public

BBa_J176071
type: SequenceAnnotation
persistent identity: annotation2158250
display id: annotation2158250
version: 1
top level: BBa_J176072
location: https://synbiohub.org/public/igem/BBa_J176072/annotation2158250/range2158250/1
component: BBa_J176071

https://synbiohub.org/public/igem/BBa_J176072/annotation2158250/range2158250/1
type: Range
persistent identity: range2158250
display id: range2158250
version: 1
top level: BBa_J176072
direction: direction/reverse
start: 1
end: 2452
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158251
display id: annotation2158251
version: 1
top level: BBa_J176072
location: https://synbiohub.org/public/igem/BBa_J176072/annotation2158251/range2158251/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_J176072/annotation2158251/range2158251/1
type: Range
persistent identity: range2158251
display id: range2158251
version: 1
top level: BBa_J176072
direction: direction/reverse
start: 2453
end: 2458
orientation: inline

BBa_S04679
type: SequenceAnnotation
persistent identity: annotation2158258
display id: annotation2158258
version: 1
top level: BBa_J176072
location: https://synbiohub.org/public/igem/BBa_J176072/annotation2158258/range2158258/1
component: BBa_S04679

https://synbiohub.org/public/igem/BBa_J176072/annotation2158258/range2158258/1
type: Range
persistent identity: range2158258
display id: range2158258
version: 1
top level: BBa_J176072
direction: direction/reverse
start: 2459
end: 3618
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa J176041
display id: BBa_J176041
version: 1
was generated by: iGEM to SBOL conversion
description: Silver scar
created: 2011-10-04T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard).
owned by: james
owned by: myers
mutable description: Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard).
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts.
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J176041_sequence/1

BBa_S04679
type: ComponentDefinition
persistent identity: BBa S04679
display id: BBa_S04679
version: 1
was generated by: iGEM to SBOL conversion
description: J176009:J176021
created: 2011-10-06T11:00:00Z
modified: 2015-05-08T01:14:43Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J176042
component: BBa_J176021
component: BBa_J176041
sequence annotation: BBa_J176042
sequence annotation: BBa_J176041
sequence annotation: BBa_J176021
sequence: https://synbiohub.org/public/igem/BBa_S04679_sequence/1

BBa_J176042
type: Component
persistent identity: component2156681
display id: component2156681
version: 1
top level: BBa_S04679
definition: polyA
access: public

BBa_J176021
type: Component
persistent identity: component2156683
display id: component2156683
version: 1
top level: BBa_S04679
definition: HS4
access: public

BBa_J176041
type: Component
persistent identity: component2156682
display id: component2156682
version: 1
top level: BBa_S04679
definition: scar
access: public

BBa_J176021
type: Component
persistent identity: component2156685
display id: component2156685
version: 1
top level: BBa_S04679
definition: HS4
access: public

BBa_J176041
type: Component
persistent identity: component2156684
display id: component2156684
version: 1
top level: BBa_S04679
definition: scar
access: public

BBa_J176042
type: SequenceAnnotation
persistent identity: annotation2156681
display id: annotation2156681
version: 1
top level: BBa_S04679
location: https://synbiohub.org/public/igem/BBa_S04679/annotation2156681/range2156681/1
component: BBa_J176042

https://synbiohub.org/public/igem/BBa_S04679/annotation2156681/range2156681/1
type: Range
persistent identity: range2156681
display id: range2156681
version: 1
top level: BBa_S04679
direction: direction/reverse
start: 1
end: 468
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156682
display id: annotation2156682
version: 1
top level: BBa_S04679
location: https://synbiohub.org/public/igem/BBa_S04679/annotation2156682/range2156682/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04679/annotation2156682/range2156682/1
type: Range
persistent identity: range2156682
display id: range2156682
version: 1
top level: BBa_S04679
direction: direction/reverse
start: 469
end: 474
orientation: inline

BBa_J176021
type: SequenceAnnotation
persistent identity: annotation2156683
display id: annotation2156683
version: 1
top level: BBa_S04679
location: https://synbiohub.org/public/igem/BBa_S04679/annotation2156683/range2156683/1
component: BBa_J176021

https://synbiohub.org/public/igem/BBa_S04679/annotation2156683/range2156683/1
type: Range
persistent identity: range2156683
display id: range2156683
version: 1
top level: BBa_S04679
direction: direction/reverse
start: 475
end: 814
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156684
display id: annotation2156684
version: 1
top level: BBa_S04679
location: https://synbiohub.org/public/igem/BBa_S04679/annotation2156684/range2156684/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04679/annotation2156684/range2156684/1
type: Range
persistent identity: range2156684
display id: range2156684
version: 1
top level: BBa_S04679
direction: direction/reverse
start: 815
end: 820
orientation: inline

BBa_J176021
type: SequenceAnnotation
persistent identity: annotation2156685
display id: annotation2156685
version: 1
top level: BBa_S04679
location: https://synbiohub.org/public/igem/BBa_S04679/annotation2156685/range2156685/1
component: BBa_J176021

https://synbiohub.org/public/igem/BBa_S04679/annotation2156685/range2156685/1
type: Range
persistent identity: range2156685
display id: range2156685
version: 1
top level: BBa_S04679
direction: direction/reverse
start: 821
end: 1160
orientation: inline

BBa_J176071
type: ComponentDefinition
persistent identity: BBa J176071
display id: BBa_J176071
version: 1
was generated by: iGEM to SBOL conversion
description: EED expression casette 1
created: 2011-10-13T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: EED expression casette 1
discontinued: false
dominant: false
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_J176041
component: BBa_S04680
component: BBa_J176024
component: BBa_S04645
sequence annotation: BBa_S04645
sequence annotation: BBa_J176041
sequence annotation: BBa_S04680
sequence annotation: BBa_J176024
sequence: https://synbiohub.org/public/igem/BBa_J176071_sequence/1

BBa_J176041
type: Component
persistent identity: component2158237
display id: component2158237
version: 1
top level: BBa_J176071
definition: scar
access: public

BBa_S04680
type: Component
persistent identity: component2158236
display id: component2158236
version: 1
top level: BBa_J176071
definition: BBa_S04680
access: public

BBa_J176024
type: Component
persistent identity: component2158238
display id: component2158238
version: 1
top level: BBa_J176071
definition: BBa_J176024
access: public

BBa_J176041
type: Component
persistent identity: component2158232
display id: component2158232
version: 1
top level: BBa_J176071
definition: scar
access: public

BBa_S04645
type: Component
persistent identity: component2158231
display id: component2158231
version: 1
top level: BBa_J176071
definition: BBa_S04645
access: public

BBa_S04645
type: SequenceAnnotation
persistent identity: annotation2158231
display id: annotation2158231
version: 1
top level: BBa_J176071
location: https://synbiohub.org/public/igem/BBa_J176071/annotation2158231/range2158231/1
component: BBa_S04645

https://synbiohub.org/public/igem/BBa_J176071/annotation2158231/range2158231/1
type: Range
persistent identity: range2158231
display id: range2158231
version: 1
top level: BBa_J176071
direction: direction/reverse
start: 1
end: 540
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158232
display id: annotation2158232
version: 1
top level: BBa_J176071
location: https://synbiohub.org/public/igem/BBa_J176071/annotation2158232/range2158232/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_J176071/annotation2158232/range2158232/1
type: Range
persistent identity: range2158232
display id: range2158232
version: 1
top level: BBa_J176071
direction: direction/reverse
start: 541
end: 546
orientation: inline

BBa_S04680
type: SequenceAnnotation
persistent identity: annotation2158236
display id: annotation2158236
version: 1
top level: BBa_J176071
location: https://synbiohub.org/public/igem/BBa_J176071/annotation2158236/range2158236/1
component: BBa_S04680

https://synbiohub.org/public/igem/BBa_J176071/annotation2158236/range2158236/1
type: Range
persistent identity: range2158236
display id: range2158236
version: 1
top level: BBa_J176071
direction: direction/reverse
start: 547
end: 2412
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158237
display id: annotation2158237
version: 1
top level: BBa_J176071
location: https://synbiohub.org/public/igem/BBa_J176071/annotation2158237/range2158237/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_J176071/annotation2158237/range2158237/1
type: Range
persistent identity: range2158237
display id: range2158237
version: 1
top level: BBa_J176071
direction: direction/reverse
start: 2413
end: 2418
orientation: inline

BBa_J176024
type: SequenceAnnotation
persistent identity: annotation2158238
display id: annotation2158238
version: 1
top level: BBa_J176071
location: https://synbiohub.org/public/igem/BBa_J176071/annotation2158238/range2158238/1
component: BBa_J176024

https://synbiohub.org/public/igem/BBa_J176071/annotation2158238/range2158238/1
type: Range
persistent identity: range2158238
display id: range2158238
version: 1
top level: BBa_J176071
direction: direction/reverse
start: 2419
end: 2452
orientation: inline

HS4
type: ComponentDefinition
persistent identity: BBa J176021
display id: BBa_J176021
version: 1
was generated by: iGEM to SBOL conversion
description: HS4
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: chicken beta-globin gene insulator
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J176021_sequence/1

BBa_J176024
type: ComponentDefinition
persistent identity: BBa J176024
display id: BBa_J176024
version: 1
was generated by: iGEM to SBOL conversion
description: NLSstop2
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: NLS followed by STOP (no scar)
discontinued: false
dominant: false
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence: https://synbiohub.org/public/igem/BBa_J176024_sequence/1

BBa_S04645
type: ComponentDefinition
persistent identity: BBa S04645
display id: BBa_S04645
version: 1
was generated by: iGEM to SBOL conversion
description: J176028:J176012
created: 2011-10-03T11:00:00Z
modified: 2015-05-08T01:14:43Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J176028
component: BBa_J176012
component: BBa_J176041
sequence annotation: BBa_J176028
sequence annotation: BBa_J176041
sequence annotation: BBa_J176012
sequence: https://synbiohub.org/public/igem/BBa_S04645_sequence/1

BBa_J176028
type: Component
persistent identity: component2154807
display id: component2154807
version: 1
top level: BBa_S04645
definition: HPK
access: public

BBa_J176012
type: Component
persistent identity: component2154809
display id: component2154809
version: 1
top level: BBa_S04645
definition: K
access: public

BBa_J176041
type: Component
persistent identity: component2154808
display id: component2154808
version: 1
top level: BBa_S04645
definition: scar
access: public

BBa_J176028
type: SequenceAnnotation
persistent identity: annotation2154807
display id: annotation2154807
version: 1
top level: BBa_S04645
location: https://synbiohub.org/public/igem/BBa_S04645/annotation2154807/range2154807/1
component: BBa_J176028

https://synbiohub.org/public/igem/BBa_S04645/annotation2154807/range2154807/1
type: Range
persistent identity: range2154807
display id: range2154807
version: 1
top level: BBa_S04645
direction: direction/reverse
start: 1
end: 516
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2154808
display id: annotation2154808
version: 1
top level: BBa_S04645
location: https://synbiohub.org/public/igem/BBa_S04645/annotation2154808/range2154808/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04645/annotation2154808/range2154808/1
type: Range
persistent identity: range2154808
display id: range2154808
version: 1
top level: BBa_S04645
direction: direction/reverse
start: 517
end: 522
orientation: inline

BBa_J176012
type: SequenceAnnotation
persistent identity: annotation2154809
display id: annotation2154809
version: 1
top level: BBa_S04645
location: https://synbiohub.org/public/igem/BBa_S04645/annotation2154809/range2154809/1
component: BBa_J176012

https://synbiohub.org/public/igem/BBa_S04645/annotation2154809/range2154809/1
type: Range
persistent identity: range2154809
display id: range2154809
version: 1
top level: BBa_S04645
direction: direction/reverse
start: 523
end: 540
orientation: inline

polyA
type: ComponentDefinition
persistent identity: BBa J176042
display id: BBa_J176042
version: 1
was generated by: iGEM to SBOL conversion
description: polyA
created: 2011-10-06T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: Originally from the Silver Lab BioBrick collection. Biological source unknown. This part was created by David Drubin.
owned by: james
owned by: myers
mutable description: Polyadenylation signal for mammalian genes
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is the first version of polyA that we used to build many of our mammalian parts, until we switched to a streamlined, shorter polyA (polyA2, BBa_J176009).
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: questionable base call
sequence: https://synbiohub.org/public/igem/BBa_J176042_sequence/1

questionable base call
type: SequenceAnnotation
persistent identity: annotation2156673
display id: annotation2156673
version: 1
top level: polyA
location: https://synbiohub.org/public/igem/BBa_J176042/annotation2156673/range2156673/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176042/annotation2156673/range2156673/1
type: Range
persistent identity: range2156673
display id: range2156673
version: 1
top level: polyA
direction: direction/forward
start: 128
end: 128
orientation: inline

BBa_S04680
type: ComponentDefinition
persistent identity: BBa S04680
display id: BBa_S04680
version: 1
was generated by: iGEM to SBOL conversion
description: J176020:J176023
created: 2011-10-06T11:00:00Z
modified: 2015-05-08T01:14:43Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J176023
component: BBa_J176041
component: BBa_J176020
sequence annotation: BBa_J176020
sequence annotation: BBa_J176041
sequence annotation: BBa_J176023
sequence: https://synbiohub.org/public/igem/BBa_S04680_sequence/1

BBa_J176023
type: Component
persistent identity: component2156114
display id: component2156114
version: 1
top level: BBa_S04680
definition: BBa_J176023
access: public

BBa_J176041
type: Component
persistent identity: component2156113
display id: component2156113
version: 1
top level: BBa_S04680
definition: scar
access: public

BBa_J176020
type: Component
persistent identity: component2156112
display id: component2156112
version: 1
top level: BBa_S04680
definition: Gal4DB
access: public

BBa_J176020
type: SequenceAnnotation
persistent identity: annotation2156112
display id: annotation2156112
version: 1
top level: BBa_S04680
location: https://synbiohub.org/public/igem/BBa_S04680/annotation2156112/range2156112/1
component: BBa_J176020

https://synbiohub.org/public/igem/BBa_S04680/annotation2156112/range2156112/1
type: Range
persistent identity: range2156112
display id: range2156112
version: 1
top level: BBa_S04680
direction: direction/reverse
start: 1
end: 459
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2156113
display id: annotation2156113
version: 1
top level: BBa_S04680
location: https://synbiohub.org/public/igem/BBa_S04680/annotation2156113/range2156113/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04680/annotation2156113/range2156113/1
type: Range
persistent identity: range2156113
display id: range2156113
version: 1
top level: BBa_S04680
direction: direction/reverse
start: 460
end: 465
orientation: inline

BBa_J176023
type: SequenceAnnotation
persistent identity: annotation2156114
display id: annotation2156114
version: 1
top level: BBa_S04680
location: https://synbiohub.org/public/igem/BBa_S04680/annotation2156114/range2156114/1
component: BBa_J176023

https://synbiohub.org/public/igem/BBa_S04680/annotation2156114/range2156114/1
type: Range
persistent identity: range2156114
display id: range2156114
version: 1
top level: BBa_S04680
direction: direction/reverse
start: 466
end: 1866
orientation: inline

K
type: ComponentDefinition
persistent identity: BBa J176012
display id: BBa_J176012
version: 1
was generated by: iGEM to SBOL conversion
description: Kozak
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: yeast/ human Kozak plus start codon
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J176012_sequence/1

Gal4DB
type: ComponentDefinition
persistent identity: BBa J176020
display id: BBa_J176020
version: 1
was generated by: iGEM to SBOL conversion
description: Gal4 DB
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Gal4 DNA binding domain (remade by KAH)
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J176020_sequence/1

BBa_J176023
type: ComponentDefinition
persistent identity: BBa J176023
display id: BBa_J176023
version: 1
was generated by: iGEM to SBOL conversion
description: EED
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Embryonic Ectoderm Development; PCR2 member; Polycomb silencing
discontinued: false
dominant: false
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000110
role: partType/Temporary
sequence: https://synbiohub.org/public/igem/BBa_J176023_sequence/1

HPK
type: ComponentDefinition
persistent identity: BBa J176028
display id: BBa_J176028
version: 1
was generated by: iGEM to SBOL conversion
description: HPK
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: mutated SpeI
sequence annotation: mutated PstI
sequence: https://synbiohub.org/public/igem/BBa_J176028_sequence/1

mutated SpeI
type: SequenceAnnotation
persistent identity: annotation2166553
display id: annotation2166553
version: 1
top level: HPK
location: https://synbiohub.org/public/igem/BBa_J176028/annotation2166553/range2166553/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J176028/annotation2166553/range2166553/1
type: Range
persistent identity: range2166553
display id: range2166553
version: 1
top level: HPK
direction: direction/forward
start: 253
end: 253
orientation: inline

mutated PstI
type: SequenceAnnotation
persistent identity: annotation2166554
display id: annotation2166554
version: 1
top level: HPK
location: https://synbiohub.org/public/igem/BBa_J176028/annotation2166554/range2166554/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J176028/annotation2166554/range2166554/1
type: Range
persistent identity: range2166554
display id: range2166554
version: 1
top level: HPK
direction: direction/forward
start: 511
end: 511
orientation: inline

https://synbiohub.org/public/igem/BBa_J176072_sequence/1
type: Sequence
persistent identity: BBa J176072 sequence
display id: BBa_J176072_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacccgccgccaccatggagactagaatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagaatgtccgagagggaagtgtcgactgcgccggcgggaacagacatgcctgcggccaagaagcagaagctgagcagtgacgagaacagcaatccagacctctctggagacgagaatgatgacgctgtcagtatagaaagtggtacaaacactgaacgccctgatacacctacaaacacgccaaatgcacctggaaggaaaagttggggaaagggaaaatggaagtcaaagaaatgcaaatattctttcaaatgtgtaaatagtctcaaggaagatcataaccaaccattgtttggagttcagtttaactggcacagtaaagaaggagatccattagtgtttgcaactgtaggaagcaacagagttaccttgtatgaatgtcattcacaaggagaaatccggttgttgcaatcttacgtggatgctgatgctgatgaaaacttttacacttgtgcatggacctatgatagcaatacgagccatcctctgctggctgtagctggatcaagaggcataattaggataataaatcctataacaatgcagtgtataaagcactatgttggccatggaaatgctatcaatgagctgaaattccatccaagagatccaaatcttctcctgtcagtaagtaaagatcatgctttacgattatggaatatccagacggacactctggtggcaatatttggaggcgtagaagggcacagagatgaagttctaagtgctgattatgatcttttgggtgaaaaaataatgtcctgtggtatggatcattctcttaaactttggaggatcaattcaaagagaatgatgaatgcaattaaggaatcttatgattataatccaaataaaactaacaggccatttatttctcagaaaatccattttcctgatttttctaccagagacatacataggaattatgttgattgtgtgcgatggttaggcgatttgatactttctaagagtggccgtgccattttacattc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176041_sequence/1
type: Sequence
persistent identity: BBa J176041 sequence
display id: BBa_J176041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04679_sequence/1
type: Sequence
persistent identity: BBa S04679 sequence
display id: BBa_S04679_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagcttactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176071_sequence/1
type: Sequence
persistent identity: BBa J176071 sequence
display id: BBa_J176071_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacccgccgccaccatggagactagaatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagaatgtccgagagggaagtgtcgactgcgccggcgggaacagacatgcctgcggccaagaagcagaagctgagcagtgacgagaacagcaatccagacctctctggagacgagaatgatgacgctgtcagtatagaaagtggtacaaacactgaacgccctgatacacctacaaacacgccaaatgcacctggaaggaaaagttggggaaagggaaaatggaagtcaaagaaatgcaaatattctttcaaatgtgtaaatagtctcaaggaagatcataaccaaccattgtttggagttcagtttaactggcacagtaaagaaggagatccattagtgtttgcaactgtaggaagcaacagagttaccttgtatgaatgtcattcacaaggagaaatccggttgttgcaatcttacgtggatgctgatgctgatgaaaacttttacacttgtgcatggacctatgatagcaatacgagccatcctctgctggctgtagctggatcaagaggcataattaggataataaatcctataacaatgcagtgtataaagcactatgttggccatggaaatgctatcaatgagctgaaattccatccaagagatccaaatcttctcctgtcagtaagtaaagatcatgctttacgattatggaatatccagacggacactctggtggcaatatttggaggcgtagaagggcacagagatgaagttctaagtgctgattatgatcttttgggtgaaaaaataatgtcctgtggtatggatcattctcttaaactttggaggatcaattcaaagagaatgatgaatgcaattaaggaatcttatgattataatccaaataaaactaacaggccatttatttctcagaaaatccattttcctgatttttctaccagagacatacataggaattatgttgattgtgtgcgatggttaggcgatttgatactttctaagagtggccgtgccattttacattc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176021_sequence/1
type: Sequence
persistent identity: BBa J176021 sequence
display id: BBa_J176021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176024_sequence/1
type: Sequence
persistent identity: BBa J176024 sequence
display id: BBa_J176024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccaagaaaaagcgcaaggtagcgtaaagctgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04645_sequence/1
type: Sequence
persistent identity: BBa S04645 sequence
display id: BBa_S04645_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176042_sequence/1
type: Sequence
persistent identity: BBa J176042 sequence
display id: BBa_J176042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04680_sequence/1
type: Sequence
persistent identity: BBa S04680 sequence
display id: BBa_S04680_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagaatgtccgagagggaagtgtcgactgcgccggcgggaacagacatgcctgcggccaagaagcagaagctgagcagtgacgagaacagcaatccagacctctctggagacgagaatgatgacgctgtcagtatagaaagtggtacaaacactgaacgccctgatacacctacaaacacgccaaatgcacctggaaggaaaagttggggaaagggaaaatggaagtcaaagaaatgcaaatattctttcaaatgtgtaaatagtctcaaggaagatcataaccaaccattgtttggagttcagtttaactggcacagtaaagaaggagatccattagtgtttgcaactgtaggaagcaacagagttaccttgtatgaatgtcattcacaaggagaaatccggttgttgcaatcttacgtggatgctgatgctgatgaaaacttttacacttgtgcatggacctatgatagcaatacgagccatcctctgctggctgtagctggatcaagaggcataattaggataataaatcctataacaatgcagtgtataaagcactatgttggccatggaaatgctatcaatgagctgaaattccatccaagagatccaaatcttctcctgtcagtaagtaaagatcatgctttacgattatggaatatccagacggacactctggtggcaatatttggaggcgtagaagggcacagagatgaagttctaagtgctgattatgatcttttgggtgaaaaaataatgtcctgtggtatggatcattctcttaaactttggaggatcaattcaaagagaatgatgaatgcaattaaggaatcttatgattataatccaaataaaactaacaggccatttatttctcagaaaatccattttcctgatttttctaccagagacatacataggaattatgttgattgtgtgcgatggttaggcgatttgatactttctaagagtggccgtgccattttacattcccaccagcaatgtatgagagatccagtgtctccgaatcttcgccagcatttgtcttgtgaaaatgccattgtgtgctggaaacctggcaagatggaagatgatatagataaaattaaacccagtgaatctaatgtgactattcttgggcgatttgattacagccagtgtgacatttggtacatgaggttttctatggatttctggcaaaagatgcttgcattgggcaatcaagttggcaaactttatgtttgggatttagaagtagaagatcctcataaagccaaatgtacaacactgactcatcataaatgtggtgctgctattcgacaaaccagttttagcagggatagcagcattcttatagctgtttgtgatgatgccagtatttggcgctgggatcgacttcgattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176012_sequence/1
type: Sequence
persistent identity: BBa J176012 sequence
display id: BBa_J176012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176020_sequence/1
type: Sequence
persistent identity: BBa J176020 sequence
display id: BBa_J176020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176023_sequence/1
type: Sequence
persistent identity: BBa J176023 sequence
display id: BBa_J176023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccgagagggaagtgtcgactgcgccggcgggaacagacatgcctgcggccaagaagcagaagctgagcagtgacgagaacagcaatccagacctctctggagacgagaatgatgacgctgtcagtatagaaagtggtacaaacactgaacgccctgatacacctacaaacacgccaaatgcacctggaaggaaaagttggggaaagggaaaatggaagtcaaagaaatgcaaatattctttcaaatgtgtaaatagtctcaaggaagatcataaccaaccattgtttggagttcagtttaactggcacagtaaagaaggagatccattagtgtttgcaactgtaggaagcaacagagttaccttgtatgaatgtcattcacaaggagaaatccggttgttgcaatcttacgtggatgctgatgctgatgaaaacttttacacttgtgcatggacctatgatagcaatacgagccatcctctgctggctgtagctggatcaagaggcataattaggataataaatcctataacaatgcagtgtataaagcactatgttggccatggaaatgctatcaatgagctgaaattccatccaagagatccaaatcttctcctgtcagtaagtaaagatcatgctttacgattatggaatatccagacggacactctggtggcaatatttggaggcgtagaagggcacagagatgaagttctaagtgctgattatgatcttttgggtgaaaaaataatgtcctgtggtatggatcattctcttaaactttggaggatcaattcaaagagaatgatgaatgcaattaaggaatcttatgattataatccaaataaaactaacaggccatttatttctcagaaaatccattttcctgatttttctaccagagacatacataggaattatgttgattgtgtgcgatggttaggcgatttgatactttctaagagtggccgtgccattttacattcccaccagcaatgtatgagagatccagtgtctccgaatcttcgccagcatttgtcttgtgaaaatgccattgtgtgctggaaacctggcaagatggaagatgatatagataaaattaaacccagtgaatctaatgtgactattcttgggcgatttgattacagccagtgtgacatttggtacatgaggttttctatggatttctggcaaaagatgcttgcattgggcaatcaagttggcaaactttatgtttgggatttagaagtagaagatcctcataaagccaaatgtacaacactgactcatcataaatgtggtgctgctattcgacaaaccagttttagcagggatagcagcattcttatagctgtttgtgatgatgccagtatttggcgctgggatcgacttcgattg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176028_sequence/1
type: Sequence
persistent identity: BBa J176028 sequence
display id: BBa_J176028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z