LOCUS       BBa_J176089             2941 bp    DNA     linear       22-Dec-2024
DEFINITION  KAH179
ACCESSION   BBa_J176089
VERSION     BBa_J176089.1  
FEATURES             Location/Qualifiers
     misc_feature    253..253
                     /label=mutated SpeI
     misc_feature    511..511
                     /label=mutated PstI
     promoter        1..516
                     /label=BBa_J176028
     misc_feature    517..522
                     /label=BBa_J176041
     misc_feature    523..539
                     /label=Gal4
     misc_feature    542..558
                     /label=Gal4
     misc_feature    561..577
                     /label=Gal4
     misc_feature    580..596
                     /label=Gal4
     misc_feature    599..615
                     /label=Gal4
     misc_feature    523..615
                     /label=BBa_J176019
     misc_feature    616..621
                     /label=BBa_J176041
     misc_feature    622..638
                     /label=Gal4
     misc_feature    641..657
                     /label=Gal4
     misc_feature    660..676
                     /label=Gal4
     misc_feature    679..695
                     /label=Gal4
     misc_feature    698..714
                     /label=Gal4
     misc_feature    622..714
                     /label=BBa_J176019
     misc_feature    1..714
                     /label=BBa_S04655
     misc_feature    715..720
                     /label=BBa_J176041
     RBS             721..738
                     /label=BBa_J176012
     misc_feature    739..744
                     /label=BBa_J176041
     misc_feature    745..1278
                     /label=BBa_J176018
     misc_feature    1279..1284
                     /label=BBa_J176041
     misc_feature    1285..1971
                     /label=BBa_J176022
     misc_feature    1972..1977
                     /label=BBa_J176041
     misc_feature    1978..2664
                     /label=BBa_J176022
     misc_feature    2665..2670
                     /label=BBa_J176041
     misc_feature    2671..2691
                     /label=BBa_J176008
     misc_feature    2692..2697
                     /label=BBa_J176041
     terminator      2698..2710
                     /label=BBa_J176016
     misc_feature    721..2710
                     /label=BBa_S04733
     misc_feature    2711..2716
                     /label=BBa_J176041
     misc_feature    2717..2941
                     /label=BBa_J176009
     misc_feature    721..2941
                     /label=BBa_S04734
ORIGIN
        1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
       61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
      121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
      181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
      241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
      301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
      361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
      421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
      481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga
      541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      601 gagtactgtc ctccgactag acggagtact gtcctccgag cggagtactg tcctccgagc
      661 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgactaga
      721 cccgccgcca ccatggagac tagagcatcc aacttgaaca tttcgagaaa gcttaccata
      781 tcaaccccat catgctcttt cgaaaattca aatagcacat ccattccttc gcccgcttcc
      841 tcatctcaaa gccacactcc aatgagaaac atgagctcac tctctgataa cagcgttttc
      901 agccggaata tggaacaatc atcaccaatc actccaagta tgtaccaatt tggtcagcag
      961 cagtcaaaca gtatatgtgg tagcaccgtt agtgtgaata gtctggtgaa tacaaataac
     1021 aaacaaagga tctacgaaca aatcacgggt cctaacagca ataacgcaac caatgattat
     1081 attgatttgc taaacctaaa tgagtctaac aaggaaaacc aaaatcccgc aacggcgcat
     1141 tacctcaatg ggggcccacc caagacaagc ttcattaacc atggaatgtt cccctcgcca
     1201 actgggacca taaatagcgg taaatctagc agtgcctcat ctttaatttc ttttggtatg
     1261 ggcaataccc aagtaataac tagaatggcc ctgtccaaca agttcatcgg cgacgacatg
     1321 aagatgacct accacatgga cggctgcgtg aacggccact acttcaccgt gaagggcgag
     1381 ggcagcggca agccctacga gggcacccag acctccacct tcaaggtgac catggccaac
     1441 ggcggccccc tggccttctc cttcgacatc ctgtccaccg tgttcatgta cggcaaccgc
     1501 tgcttcaccg cctaccccac cagcatgccc gactacttca agcaggcctt ccccgacggc
     1561 atgtcctacg agagaacctt cacctacgag gacggcggcg tggccaccgc cagctgggag
     1621 atcagcctga agggcaactg cttcgagcac aagtccacct tccacggcgt gaacttcccc
     1681 gccgacggcc ccgtgatggc caagaagacc accggctggg acccctcctt cgagaagatg
     1741 accgtgtgcg acggcatctt gaagggcgac gtgaccgcct tcctgatgct gcaaggcggc
     1801 ggcaactaca gatgccagtt ccacacctcc tacaagacca agaagcccgt gaccatgccc
     1861 cccaaccacg tggtggagca ccgcatcgcc agaaccgacc tggacaaggg cggcaacagc
     1921 gtgcagctga ccgagcacgc cgtggcccac atcacctccg tggtgccctt cactagaatg
     1981 gccctgtcca acaagttcat cggcgacgac atgaagatga cctaccacat ggacggctgc
     2041 gtgaacggcc actacttcac cgtgaagggc gagggcagcg gcaagcccta cgagggcacc
     2101 cagacctcca ccttcaaggt gaccatggcc aacggcggcc ccctggcctt ctccttcgac
     2161 atcctgtcca ccgtgttcat gtacggcaac cgctgcttca ccgcctaccc caccagcatg
     2221 cccgactact tcaagcaggc cttccccgac ggcatgtcct acgagagaac cttcacctac
     2281 gaggacggcg gcgtggccac cgccagctgg gagatcagcc tgaagggcaa ctgcttcgag
     2341 cacaagtcca ccttccacgg cgtgaacttc cccgccgacg gccccgtgat ggccaagaag
     2401 accaccggct gggacccctc cttcgagaag atgaccgtgt gcgacggcat cttgaagggc
     2461 gacgtgaccg ccttcctgat gctgcaaggc ggcggcaact acagatgcca gttccacacc
     2521 tcctacaaga ccaagaagcc cgtgaccatg ccccccaacc acgtggtgga gcaccgcatc
     2581 gccagaaccg acctggacaa gggcggcaac agcgtgcagc tgaccgagca cgccgtggcc
     2641 cacatcacct ccgtggtgcc cttcactaga cccaagaaaa agcgcaaggt aactagagcg
     2701 taaagctgag actagactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2761 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     2821 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     2881 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2941 g
//