BBa_J176089
type: ComponentDefinition
persistent identity: BBa J176089
display id: BBa_J176089
version: 1
was generated by: iGEM to SBOL conversion
description: KAH179
created: 2011-10-14T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Note: remake w/o PEST
discontinued: false
dominant: false
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Temporary
role: SO:0000110
component: BBa_S04655
component: BBa_S04734
component: BBa_J176041
sequence annotation: BBa_S04655
sequence annotation: BBa_J176041
sequence annotation: BBa_S04734
sequence: https://synbiohub.org/public/igem/BBa_J176089_sequence/1

BBa_S04655
type: Component
persistent identity: component2158713
display id: component2158713
version: 1
top level: BBa_J176089
definition: BBa_S04655
access: public

BBa_S04734
type: Component
persistent identity: component2158729
display id: component2158729
version: 1
top level: BBa_J176089
definition: BBa_S04734
access: public

BBa_J176041
type: Component
persistent identity: component2158714
display id: component2158714
version: 1
top level: BBa_J176089
definition: scar
access: public

BBa_S04655
type: SequenceAnnotation
persistent identity: annotation2158713
display id: annotation2158713
version: 1
top level: BBa_J176089
location: https://synbiohub.org/public/igem/BBa_J176089/annotation2158713/range2158713/1
component: BBa_S04655

https://synbiohub.org/public/igem/BBa_J176089/annotation2158713/range2158713/1
type: Range
persistent identity: range2158713
display id: range2158713
version: 1
top level: BBa_J176089
direction: direction/reverse
start: 1
end: 714
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158714
display id: annotation2158714
version: 1
top level: BBa_J176089
location: https://synbiohub.org/public/igem/BBa_J176089/annotation2158714/range2158714/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_J176089/annotation2158714/range2158714/1
type: Range
persistent identity: range2158714
display id: range2158714
version: 1
top level: BBa_J176089
direction: direction/reverse
start: 715
end: 720
orientation: inline

BBa_S04734
type: SequenceAnnotation
persistent identity: annotation2158729
display id: annotation2158729
version: 1
top level: BBa_J176089
location: https://synbiohub.org/public/igem/BBa_J176089/annotation2158729/range2158729/1
component: BBa_S04734

https://synbiohub.org/public/igem/BBa_J176089/annotation2158729/range2158729/1
type: Range
persistent identity: range2158729
display id: range2158729
version: 1
top level: BBa_J176089
direction: direction/reverse
start: 721
end: 2941
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa J176041
display id: BBa_J176041
version: 1
was generated by: iGEM to SBOL conversion
description: Silver scar
created: 2011-10-04T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard).
owned by: james
owned by: myers
mutable description: Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard).
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts.
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J176041_sequence/1

BBa_S04655
type: ComponentDefinition
persistent identity: BBa S04655
display id: BBa_S04655
version: 1
was generated by: iGEM to SBOL conversion
description: J176028:J176019
created: 2011-10-05T11:00:00Z
modified: 2015-05-08T01:14:43Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J176028
component: BBa_J176019
component: BBa_J176041
sequence annotation: BBa_J176028
sequence annotation: BBa_J176041
sequence annotation: BBa_J176019
sequence: https://synbiohub.org/public/igem/BBa_S04655_sequence/1

BBa_J176028
type: Component
persistent identity: component2155738
display id: component2155738
version: 1
top level: BBa_S04655
definition: HPK
access: public

BBa_J176019
type: Component
persistent identity: component2155740
display id: component2155740
version: 1
top level: BBa_S04655
definition: 5xGal4
access: public

BBa_J176041
type: Component
persistent identity: component2155739
display id: component2155739
version: 1
top level: BBa_S04655
definition: scar
access: public

BBa_J176019
type: Component
persistent identity: component2155742
display id: component2155742
version: 1
top level: BBa_S04655
definition: 5xGal4
access: public

BBa_J176041
type: Component
persistent identity: component2155741
display id: component2155741
version: 1
top level: BBa_S04655
definition: scar
access: public

BBa_J176028
type: SequenceAnnotation
persistent identity: annotation2155738
display id: annotation2155738
version: 1
top level: BBa_S04655
location: https://synbiohub.org/public/igem/BBa_S04655/annotation2155738/range2155738/1
component: BBa_J176028

https://synbiohub.org/public/igem/BBa_S04655/annotation2155738/range2155738/1
type: Range
persistent identity: range2155738
display id: range2155738
version: 1
top level: BBa_S04655
direction: direction/reverse
start: 1
end: 516
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2155739
display id: annotation2155739
version: 1
top level: BBa_S04655
location: https://synbiohub.org/public/igem/BBa_S04655/annotation2155739/range2155739/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04655/annotation2155739/range2155739/1
type: Range
persistent identity: range2155739
display id: range2155739
version: 1
top level: BBa_S04655
direction: direction/reverse
start: 517
end: 522
orientation: inline

BBa_J176019
type: SequenceAnnotation
persistent identity: annotation2155740
display id: annotation2155740
version: 1
top level: BBa_S04655
location: https://synbiohub.org/public/igem/BBa_S04655/annotation2155740/range2155740/1
component: BBa_J176019

https://synbiohub.org/public/igem/BBa_S04655/annotation2155740/range2155740/1
type: Range
persistent identity: range2155740
display id: range2155740
version: 1
top level: BBa_S04655
direction: direction/reverse
start: 523
end: 615
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2155741
display id: annotation2155741
version: 1
top level: BBa_S04655
location: https://synbiohub.org/public/igem/BBa_S04655/annotation2155741/range2155741/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04655/annotation2155741/range2155741/1
type: Range
persistent identity: range2155741
display id: range2155741
version: 1
top level: BBa_S04655
direction: direction/reverse
start: 616
end: 621
orientation: inline

BBa_J176019
type: SequenceAnnotation
persistent identity: annotation2155742
display id: annotation2155742
version: 1
top level: BBa_S04655
location: https://synbiohub.org/public/igem/BBa_S04655/annotation2155742/range2155742/1
component: BBa_J176019

https://synbiohub.org/public/igem/BBa_S04655/annotation2155742/range2155742/1
type: Range
persistent identity: range2155742
display id: range2155742
version: 1
top level: BBa_S04655
direction: direction/reverse
start: 622
end: 714
orientation: inline

BBa_S04734
type: ComponentDefinition
persistent identity: BBa S04734
display id: BBa_S04734
version: 1
was generated by: iGEM to SBOL conversion
description: J176041:J176009
created: 2011-10-14T11:00:00Z
modified: 2015-05-08T01:14:44Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S04733
component: BBa_J176009
component: BBa_J176041
sequence annotation: BBa_S04733
sequence annotation: BBa_J176041
sequence annotation: BBa_J176009
sequence: https://synbiohub.org/public/igem/BBa_S04734_sequence/1

BBa_S04733
type: Component
persistent identity: component2158573
display id: component2158573
version: 1
top level: BBa_S04734
definition: BBa_S04733
access: public

BBa_J176009
type: Component
persistent identity: component2158575
display id: component2158575
version: 1
top level: BBa_S04734
definition: polyA
access: public

BBa_J176041
type: Component
persistent identity: component2158574
display id: component2158574
version: 1
top level: BBa_S04734
definition: scar
access: public

BBa_S04733
type: SequenceAnnotation
persistent identity: annotation2158573
display id: annotation2158573
version: 1
top level: BBa_S04734
location: https://synbiohub.org/public/igem/BBa_S04734/annotation2158573/range2158573/1
component: BBa_S04733

https://synbiohub.org/public/igem/BBa_S04734/annotation2158573/range2158573/1
type: Range
persistent identity: range2158573
display id: range2158573
version: 1
top level: BBa_S04734
direction: direction/reverse
start: 1
end: 1990
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158574
display id: annotation2158574
version: 1
top level: BBa_S04734
location: https://synbiohub.org/public/igem/BBa_S04734/annotation2158574/range2158574/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04734/annotation2158574/range2158574/1
type: Range
persistent identity: range2158574
display id: range2158574
version: 1
top level: BBa_S04734
direction: direction/reverse
start: 1991
end: 1996
orientation: inline

BBa_J176009
type: SequenceAnnotation
persistent identity: annotation2158575
display id: annotation2158575
version: 1
top level: BBa_S04734
location: https://synbiohub.org/public/igem/BBa_S04734/annotation2158575/range2158575/1
component: BBa_J176009

https://synbiohub.org/public/igem/BBa_S04734/annotation2158575/range2158575/1
type: Range
persistent identity: range2158575
display id: range2158575
version: 1
top level: BBa_S04734
direction: direction/reverse
start: 1997
end: 2221
orientation: inline

polyA
type: ComponentDefinition
persistent identity: BBa J176009
display id: BBa_J176009
version: 1
was generated by: iGEM to SBOL conversion
description: polyA2
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: BGH polyA signal cloned from pcDNA5/FRT vector
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176009_sequence/1

5xGal4
type: ComponentDefinition
persistent identity: BBa J176019
display id: BBa_J176019
version: 1
was generated by: iGEM to SBOL conversion
description: 5xGal4
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: target sequence of GAL4 DB; PCR cloned from pNEB
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Gal4
sequence: https://synbiohub.org/public/igem/BBa_J176019_sequence/1

Gal4
type: SequenceAnnotation
persistent identity: annotation2159559
display id: annotation2159559
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159559/range2159559/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J176019/annotation2159559/range2159559/1
type: Range
persistent identity: range2159559
display id: range2159559
version: 1
top level: 5xGal4
direction: direction/forward
start: 1
end: 17
orientation: inline

Gal4
type: SequenceAnnotation
persistent identity: annotation2159560
display id: annotation2159560
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159560/range2159560/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176019/annotation2159560/range2159560/1
type: Range
persistent identity: range2159560
display id: range2159560
version: 1
top level: 5xGal4
direction: direction/forward
start: 20
end: 36
orientation: inline

Gal4
type: SequenceAnnotation
persistent identity: annotation2159561
display id: annotation2159561
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159561/range2159561/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J176019/annotation2159561/range2159561/1
type: Range
persistent identity: range2159561
display id: range2159561
version: 1
top level: 5xGal4
direction: direction/forward
start: 39
end: 55
orientation: inline

Gal4
type: SequenceAnnotation
persistent identity: annotation2159562
display id: annotation2159562
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159562/range2159562/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176019/annotation2159562/range2159562/1
type: Range
persistent identity: range2159562
display id: range2159562
version: 1
top level: 5xGal4
direction: direction/forward
start: 58
end: 74
orientation: inline

Gal4
type: SequenceAnnotation
persistent identity: annotation2159563
display id: annotation2159563
version: 1
top level: 5xGal4
location: https://synbiohub.org/public/igem/BBa_J176019/annotation2159563/range2159563/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176019/annotation2159563/range2159563/1
type: Range
persistent identity: range2159563
display id: range2159563
version: 1
top level: 5xGal4
direction: direction/forward
start: 77
end: 93
orientation: inline

HPK
type: ComponentDefinition
persistent identity: BBa J176028
display id: BBa_J176028
version: 1
was generated by: iGEM to SBOL conversion
description: HPK
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: mutated SpeI
sequence annotation: mutated PstI
sequence: https://synbiohub.org/public/igem/BBa_J176028_sequence/1

mutated SpeI
type: SequenceAnnotation
persistent identity: annotation2166553
display id: annotation2166553
version: 1
top level: HPK
location: https://synbiohub.org/public/igem/BBa_J176028/annotation2166553/range2166553/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J176028/annotation2166553/range2166553/1
type: Range
persistent identity: range2166553
display id: range2166553
version: 1
top level: HPK
direction: direction/forward
start: 253
end: 253
orientation: inline

mutated PstI
type: SequenceAnnotation
persistent identity: annotation2166554
display id: annotation2166554
version: 1
top level: HPK
location: https://synbiohub.org/public/igem/BBa_J176028/annotation2166554/range2166554/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J176028/annotation2166554/range2166554/1
type: Range
persistent identity: range2166554
display id: range2166554
version: 1
top level: HPK
direction: direction/forward
start: 511
end: 511
orientation: inline

BBa_S04733
type: ComponentDefinition
persistent identity: BBa S04733
display id: BBa_S04733
version: 1
was generated by: iGEM to SBOL conversion
description: J176012:J176018
created: 2011-10-14T11:00:00Z
modified: 2015-05-08T01:14:44Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J176041
component: BBa_J176008
component: BBa_J176016
component: BBa_J176022
component: BBa_J176012
component: BBa_J176018
sequence annotation: BBa_J176012
sequence annotation: BBa_J176041
sequence annotation: BBa_J176018
sequence annotation: BBa_J176022
sequence annotation: BBa_J176008
sequence annotation: BBa_J176016
sequence: https://synbiohub.org/public/igem/BBa_S04733_sequence/1

BBa_J176041
type: Component
persistent identity: component2158557
display id: component2158557
version: 1
top level: BBa_S04733
definition: scar
access: public

BBa_J176008
type: Component
persistent identity: component2158556
display id: component2158556
version: 1
top level: BBa_S04733
definition: NLS
access: public

BBa_J176016
type: Component
persistent identity: component2158558
display id: component2158558
version: 1
top level: BBa_S04733
definition: STOP
access: public

BBa_J176041
type: Component
persistent identity: component2158553
display id: component2158553
version: 1
top level: BBa_S04733
definition: scar
access: public

BBa_J176022
type: Component
persistent identity: component2158552
display id: component2158552
version: 1
top level: BBa_S04733
definition: AmCyan
access: public

BBa_J176041
type: Component
persistent identity: component2158555
display id: component2158555
version: 1
top level: BBa_S04733
definition: scar
access: public

BBa_J176022
type: Component
persistent identity: component2158554
display id: component2158554
version: 1
top level: BBa_S04733
definition: AmCyan
access: public

BBa_J176041
type: Component
persistent identity: component2158549
display id: component2158549
version: 1
top level: BBa_S04733
definition: scar
access: public

BBa_J176012
type: Component
persistent identity: component2158548
display id: component2158548
version: 1
top level: BBa_S04733
definition: K
access: public

BBa_J176041
type: Component
persistent identity: component2158551
display id: component2158551
version: 1
top level: BBa_S04733
definition: scar
access: public

BBa_J176018
type: Component
persistent identity: component2158550
display id: component2158550
version: 1
top level: BBa_S04733
definition: PEST
access: public

BBa_J176012
type: SequenceAnnotation
persistent identity: annotation2158548
display id: annotation2158548
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158548/range2158548/1
component: BBa_J176012

https://synbiohub.org/public/igem/BBa_S04733/annotation2158548/range2158548/1
type: Range
persistent identity: range2158548
display id: range2158548
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 1
end: 18
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158549
display id: annotation2158549
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158549/range2158549/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04733/annotation2158549/range2158549/1
type: Range
persistent identity: range2158549
display id: range2158549
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 19
end: 24
orientation: inline

BBa_J176018
type: SequenceAnnotation
persistent identity: annotation2158550
display id: annotation2158550
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158550/range2158550/1
component: BBa_J176018

https://synbiohub.org/public/igem/BBa_S04733/annotation2158550/range2158550/1
type: Range
persistent identity: range2158550
display id: range2158550
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 25
end: 558
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158551
display id: annotation2158551
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158551/range2158551/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04733/annotation2158551/range2158551/1
type: Range
persistent identity: range2158551
display id: range2158551
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 559
end: 564
orientation: inline

BBa_J176022
type: SequenceAnnotation
persistent identity: annotation2158552
display id: annotation2158552
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158552/range2158552/1
component: BBa_J176022

https://synbiohub.org/public/igem/BBa_S04733/annotation2158552/range2158552/1
type: Range
persistent identity: range2158552
display id: range2158552
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 565
end: 1251
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158553
display id: annotation2158553
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158553/range2158553/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04733/annotation2158553/range2158553/1
type: Range
persistent identity: range2158553
display id: range2158553
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 1252
end: 1257
orientation: inline

BBa_J176022
type: SequenceAnnotation
persistent identity: annotation2158554
display id: annotation2158554
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158554/range2158554/1
component: BBa_J176022

https://synbiohub.org/public/igem/BBa_S04733/annotation2158554/range2158554/1
type: Range
persistent identity: range2158554
display id: range2158554
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 1258
end: 1944
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158555
display id: annotation2158555
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158555/range2158555/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04733/annotation2158555/range2158555/1
type: Range
persistent identity: range2158555
display id: range2158555
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 1945
end: 1950
orientation: inline

BBa_J176008
type: SequenceAnnotation
persistent identity: annotation2158556
display id: annotation2158556
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158556/range2158556/1
component: BBa_J176008

https://synbiohub.org/public/igem/BBa_S04733/annotation2158556/range2158556/1
type: Range
persistent identity: range2158556
display id: range2158556
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 1951
end: 1971
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158557
display id: annotation2158557
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158557/range2158557/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04733/annotation2158557/range2158557/1
type: Range
persistent identity: range2158557
display id: range2158557
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 1972
end: 1977
orientation: inline

BBa_J176016
type: SequenceAnnotation
persistent identity: annotation2158558
display id: annotation2158558
version: 1
top level: BBa_S04733
location: https://synbiohub.org/public/igem/BBa_S04733/annotation2158558/range2158558/1
component: BBa_J176016

https://synbiohub.org/public/igem/BBa_S04733/annotation2158558/range2158558/1
type: Range
persistent identity: range2158558
display id: range2158558
version: 1
top level: BBa_S04733
direction: direction/reverse
start: 1978
end: 1990
orientation: inline

NLS
type: ComponentDefinition
persistent identity: BBa J176008
display id: BBa_J176008
version: 1
was generated by: iGEM to SBOL conversion
description: NLS
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: SV40 nuclear localization sequence
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176008_sequence/1

K
type: ComponentDefinition
persistent identity: BBa J176012
display id: BBa_J176012
version: 1
was generated by: iGEM to SBOL conversion
description: Kozak
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: yeast/ human Kozak plus start codon
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J176012_sequence/1

STOP
type: ComponentDefinition
persistent identity: BBa J176016
display id: BBa_J176016
version: 1
was generated by: iGEM to SBOL conversion
description: STOP
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: contains stop codons TAA and TGA in frame
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J176016_sequence/1

PEST
type: ComponentDefinition
persistent identity: BBa J176018
display id: BBa_J176018
version: 1
was generated by: iGEM to SBOL conversion
description: PEST
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: degradation tag
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176018_sequence/1

AmCyan
type: ComponentDefinition
persistent identity: BBa J176022
display id: BBa_J176022
version: 1
was generated by: iGEM to SBOL conversion
description: AmCyan
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: cyan fluorescent protein from PamCyan-1C1
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176022_sequence/1

https://synbiohub.org/public/igem/BBa_J176089_sequence/1
type: Sequence
persistent identity: BBa J176089 sequence
display id: BBa_J176089_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacccgccgccaccatggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176041_sequence/1
type: Sequence
persistent identity: BBa J176041 sequence
display id: BBa_J176041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04655_sequence/1
type: Sequence
persistent identity: BBa S04655 sequence
display id: BBa_S04655_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04734_sequence/1
type: Sequence
persistent identity: BBa S04734 sequence
display id: BBa_S04734_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176009_sequence/1
type: Sequence
persistent identity: BBa J176009 sequence
display id: BBa_J176009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176019_sequence/1
type: Sequence
persistent identity: BBa J176019 sequence
display id: BBa_J176019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176028_sequence/1
type: Sequence
persistent identity: BBa J176028 sequence
display id: BBa_J176028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04733_sequence/1
type: Sequence
persistent identity: BBa S04733 sequence
display id: BBa_S04733_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176008_sequence/1
type: Sequence
persistent identity: BBa J176008 sequence
display id: BBa_J176008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccaagaaaaagcgcaaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176012_sequence/1
type: Sequence
persistent identity: BBa J176012 sequence
display id: BBa_J176012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176016_sequence/1
type: Sequence
persistent identity: BBa J176016 sequence
display id: BBa_J176016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaaagctgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176018_sequence/1
type: Sequence
persistent identity: BBa J176018 sequence
display id: BBa_J176018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176022_sequence/1
type: Sequence
persistent identity: BBa J176022 sequence
display id: BBa_J176022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z