##gff-version 3
##sequence-region BBa_J176090 1 2743
BBa_J176090	.	sequence_feature	517	522	.	+	0	ID=BBa_J176041;Name=BBa_J176041
BBa_J176090	.	promoter	1	516	.	+	0	ID=BBa_J176028;Name=BBa_J176028
BBa_J176090	.	sequence_alteration	511	511	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176090	.	sequence_alteration	253	253	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176090	.	engineered_region	523	2743	.	+	0	ID=BBa_S04734;Name=BBa_S04734
BBa_J176090	.	engineered_region	523	2512	.	+	0	ID=BBa_S04733;Name=BBa_S04733;Parent=BBa_S04734
BBa_J176090	.	polypeptide_domain	1780	2466	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04733
BBa_J176090	.	polypeptide_domain	1087	1773	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04733
BBa_J176090	.	sequence_feature	1774	1779	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04733
BBa_J176090	.	ribosome_entry_site	523	540	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04733
BBa_J176090	.	sequence_feature	541	546	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04733
BBa_J176090	.	tag	547	1080	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_S04733
BBa_J176090	.	sequence_feature	1081	1086	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04733
BBa_J176090	.	terminator	2500	2512	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04733
BBa_J176090	.	sequence_feature	2467	2472	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04733
BBa_J176090	.	tag	2473	2493	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04733
BBa_J176090	.	sequence_feature	2494	2499	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04733
BBa_J176090	.	tag	2519	2743	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04734
BBa_J176090	.	sequence_feature	2513	2518	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04734
>BBa_J176090
ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctac
acaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcg
cgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatgga
agtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggc
caatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggc
tcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct
gttctcctcttcctcatctccgggcctttcgacctggagcccactagacccgccgccaccatggagactagagcatcca
acttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttc
gcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaat
atggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccg
ttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgc
aaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctc
aatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaat
ctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagaatggccctgtccaacaagtt
catcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggc
agcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctcct
tcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaa
gcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggag
atcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatgg
ccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgc
cttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatg
ccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagc
acgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaa
gatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgag
ggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccg
tgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacgg
catgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaac
tgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggct
gggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaagg
cggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtg
gagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatca
cctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgtgccttc
tagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcc
taataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagca
agggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg