BBa_J176096 1 BBa_J176096 HPK-5xGal4-CFP 2011-10-14T11:00:00Z 2015-08-31T04:08:34Z TBA KAH189 false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes component2158903 1 BBa_S04739 component2158889 1 BBa_J176041 component2158888 1 BBa_S04643 annotation2158888 1 BBa_S04643 range2158888 1 1 615 annotation2158889 1 BBa_J176041 range2158889 1 616 621 annotation2158903 1 BBa_S04739 range2158903 1 622 2302 BBa_J176009 1 polyA polyA2 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA BGH polyA signal cloned from pcDNA5/FRT vector false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176019 1 5xGal4 5xGal4 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA target sequence of GAL4 DB; PCR cloned from pNEB false false _863_ 0 10865 9 Not in stock false TBA false Brady Laughlin annotation2159561 1 Gal4 range2159561 1 39 55 annotation2159563 1 Gal4 range2159563 1 77 93 annotation2159562 1 Gal4 range2159562 1 58 74 annotation2159559 1 Gal4 range2159559 1 1 17 annotation2159560 1 Gal4 range2159560 1 20 36 BBa_J176016 1 STOP STOP 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA contains stop codons TAA and TGA in frame false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_S04738 1 BBa_S04738 J176012:J176041 2011-10-14T11:00:00Z 2015-05-08T01:14:44Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2158749 1 BBa_J176012 component2158758 1 BBa_S04684 component2158750 1 BBa_J176041 annotation2158749 1 BBa_J176012 range2158749 1 1 18 annotation2158758 1 BBa_S04684 range2158758 1 25 1450 annotation2158750 1 BBa_J176041 range2158750 1 19 24 BBa_J176012 1 K Kozak 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 yeast/ human Kozak plus start codon false false _863_ 0 10865 9 In stock false TBA false Brady Laughlin BBa_J176041 1 scar Silver scar 2011-10-04T11:00:00Z 2015-08-31T04:08:34Z This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard). Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard). false false _863_ 0 1144 61 Not in stock false The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts. false Karmella Haynes BBa_J176028 1 HPK HPK 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166554 1 mutated PstI range2166554 1 511 511 annotation2166553 1 mutated SpeI range2166553 1 253 253 BBa_S04739 1 BBa_S04739 J176041:J176041 2011-10-14T11:00:00Z 2015-05-08T01:14:44Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2158786 1 BBa_J176041 component2158785 1 BBa_S04738 component2158787 1 BBa_J176009 annotation2158787 1 BBa_J176009 range2158787 1 1457 1681 annotation2158786 1 BBa_J176041 range2158786 1 1451 1456 annotation2158785 1 BBa_S04738 range2158785 1 1 1450 BBa_S04643 1 BBa_S04643 J176028:J176019 2011-10-03T11:00:00Z 2015-05-08T01:14:43Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2154821 1 BBa_J176041 component2154822 1 BBa_J176019 component2154820 1 BBa_J176028 annotation2154820 1 BBa_J176028 range2154820 1 1 516 annotation2154822 1 BBa_J176019 range2154822 1 523 615 annotation2154821 1 BBa_J176041 range2154821 1 517 522 BBa_J176022 1 AmCyan AmCyan 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA cyan fluorescent protein from PamCyan-1C1 false false _863_ 0 10865 9 It's complicated false TBA false Karmella Haynes BBa_J176008 1 NLS NLS 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 SV40 nuclear localization sequence false false _863_ 0 10865 9 In stock false TBA false Karmella Haynes BBa_S04684 1 BBa_S04684 J176022:J176022 2011-10-06T11:00:00Z 2015-05-08T01:14:43Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2156210 1 BBa_J176041 component2156207 1 BBa_J176022 component2156208 1 BBa_J176041 component2156209 1 BBa_J176022 component2156213 1 BBa_J176016 component2156212 1 BBa_J176041 component2156211 1 BBa_J176008 annotation2156212 1 BBa_J176041 range2156212 1 1408 1413 annotation2156211 1 BBa_J176008 range2156211 1 1387 1407 annotation2156207 1 BBa_J176022 range2156207 1 1 687 annotation2156208 1 BBa_J176041 range2156208 1 688 693 annotation2156209 1 BBa_J176022 range2156209 1 694 1380 annotation2156210 1 BBa_J176041 range2156210 1 1381 1386 annotation2156213 1 BBa_J176016 range2156213 1 1414 1426 BBa_S04739_sequence 1 cccgccgccaccatggagactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176041_sequence 1 actaga BBa_J176009_sequence 1 ctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_S04738_sequence 1 cccgccgccaccatggagactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgag BBa_J176016_sequence 1 gcgtaaagctgag BBa_J176012_sequence 1 cccgccgccaccatggag BBa_J176096_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacccgccgccaccatggagactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176022_sequence 1 atggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttc BBa_J176028_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc BBa_S04684_sequence 1 atggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgag BBa_J176019_sequence 1 cggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccg BBa_S04643_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccg BBa_J176008_sequence 1 cccaagaaaaagcgcaaggta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z