LOCUS BBa_J176121 4442 bp DNA linear 27-Dec-2024 DEFINITION V0200 ACCESSION BBa_J176121 VERSION BBa_J176121.1 FEATURES Location/Qualifiers primer_bind 205..228 /label=3t3 2.0 misc_feature 305..335 /label=NotI SpeI primer_bind 430..447 /label=BGH polyA_site 436..660 /label=BGH misc_feature 943..980 /label=FRT CDS 998..1990 /label=Hygromycin resistance polyA_site 2150..2280 /label=SV40 early polyadenylation signal misc_feature 2663..3336 /label=pUC Origin CDS 3481..4341 /label=Ampicillin resistance promoter 4342..4440 /label=bla ORIGIN 1 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 61 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct 121 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag 181 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct 241 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactaggc ttttgcaaaa 301 agctcaccgc ggccgcagtg tcaagggtac tagtcggccc gcggttcgaa ggtaagccta 361 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga gtttaaaccc 421 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 481 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 541 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 601 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 661 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 721 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 781 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 841 cccgtcaagc tctaaatcgg gggtcccttt agggttccga tttagtgctt tacggcacct 901 cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag 961 ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac 1021 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 1081 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 1141 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 1201 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 1261 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 1321 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca 1381 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 1441 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 1501 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 1561 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 1621 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 1681 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 1741 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 1801 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 1861 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 1921 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 1981 aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga 2041 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 2101 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 2161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 2221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 2281 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 2341 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 2401 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 2461 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 2521 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 2581 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2641 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2701 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2761 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2821 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2881 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2941 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3001 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3061 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3121 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 3181 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3241 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3301 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3361 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3421 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 3481 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 3541 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 3601 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 3661 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 3721 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 3781 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 3841 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 3901 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 3961 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 4021 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 4081 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 4141 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 4201 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 4261 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 4321 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 4381 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 4441 tc //