LOCUS BBa_J176122 4529 bp DNA linear 26-Nov-2024 DEFINITION pcDNA3.1+ puro ACCESSION BBa_J176122 VERSION BBa_J176122.1 FEATURES Location/Qualifiers primer_bind 186..203 /label=DD122B misc_feature 232..249 /label=pCMV remnant misc_feature 250..324 /label=SpeI EcoRI PstI NotI XbaI misc_feature 320..322 /label=STOP in frame w/ XbaI misc_feature 329..331 /label=STOP in frame w/ XbaI primer_bind 336..353 /label=BGH polyA_site 342..566 /label=BGH primer_bind 344..362 /label=DD123B misc_feature 612..1040 /label=f1 origin promoter 1045..1389 /label=SV40 CDS 1393..1992 /label=Puromycin resistance polyA_site 2206..2335 /label=SV40 CDS 3533..4393 /label=Ampicillin resistance promoter 4394..4492 /label=bla ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct 301 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 361 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 421 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 481 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 541 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 601 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 841 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 901 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 961 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 1021 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 1081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 1141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 1201 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 1261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 1321 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 1381 aaaaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 1441 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 1501 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 1561 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 1621 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 1681 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 1741 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 1801 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 1861 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 1921 accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag 1981 cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc 2041 catgcatcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 2101 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 2161 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 2221 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 2281 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 2341 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 2401 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 2461 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 2521 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 2581 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 2641 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 2701 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 2761 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 2821 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 2881 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 2941 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 3001 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3061 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 3121 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3181 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 3241 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3301 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 3361 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 3421 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 3481 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 3541 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 3601 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 3661 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 3721 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 3781 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 3841 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 3901 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 3961 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 4021 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 4081 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 4141 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 4201 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 4261 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 4321 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 4381 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 4441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 4501 catttccccg aaaagtgcca cctgacgtc //