LOCUS BBa_J176123 4952 bp DNA linear 06-Dec-2025 DEFINITION pcDNA3.1+ hygro ACCESSION BBa_J176123 VERSION BBa_J176123.1 FEATURES Location/Qualifiers misc_feature 232..249 /label=CMV promoter remnant misc_feature 250..324 /label=SpeI NotI XbaI primer_bind 336..353 /label=BGH polyA_site 342..566 /label=BGH misc_feature 612..1040 /label=f1 origin promoter 1045..1388 /label=SV40 CDS 1438..2468 /label=Hygromycin resistance polyA_site 2628..2758 /label=SV40 misc_feature 3141..3811 /label=pUC origin CDS 3956..4816 /label=Ampicillin resistance promoter 4816..4915 /label=bla primer_bind 186..203 /label=DD122B ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct 301 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 361 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 421 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 481 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 541 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 601 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 841 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 901 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 961 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 1021 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 1081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 1141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 1201 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 1261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 1321 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 1381 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 1441 tcatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg 1501 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg 1561 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag 1621 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca 1681 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt 1741 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg 1801 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag 1861 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt 1921 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg 1981 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg 2041 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg 2101 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg 2161 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc 2221 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg 2281 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg 2341 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg 2401 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa 2461 aggaatagtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 2521 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 2581 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 2641 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 2701 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 2761 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 2821 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 2881 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 2941 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 3001 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 3061 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 3121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 3181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 3241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 3301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 3361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 3421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 3481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 3541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 3601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 3661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3721 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 3781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 3841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 3901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 3961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 4321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 4381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 4441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 4501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 4561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 4681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 4741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 4801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 4861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 4921 gcacatttcc ccgaaaagtg ccacctgacg tc //