LOCUS BBa_J176124 4594 bp DNA linear 23-Dec-2024 DEFINITION pcDNA3.1+ zeo ACCESSION BBa_J176124 VERSION BBa_J176124.1 FEATURES Location/Qualifiers misc_feature 232..249 /label=CMV promoter remnant misc_feature 250..324 /label=SpeI EcoRI PstI NotI XbaI primer_bind 336..353 /label=BGH polyA_site 342..566 /label=BGH misc_feature 612..1040 /label=f1 origin promoter 1045..1388 /label=SV40 promoter 1548..1614 /label=EM7 CDS 1615..1989 /label=Zeocin resistance polyA_site 2003..2084 /label=SV40 polyA_site 2270..2400 /label=SV40 misc_feature 2783..3453 /label=pUC origin CDS 3598..4458 /label=Ampicillin resistance promoter 4459..4557 /label=bla primer_bind 186..203 /label=DD122B ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct 301 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 361 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 421 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 481 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 541 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 601 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 841 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 901 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 961 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 1021 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 1081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 1141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 1201 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 1261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 1321 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 1381 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 1441 tcccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg 1501 gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cggaccgtgt tgacaattaa 1561 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc 1621 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc 1681 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc 1741 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc 1801 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg 1861 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg 1921 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag 1981 caggactgac actcgacctc gaaacttgtt tattgcagct tataatggtt acaaataaag 2041 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 2101 gtccaaactc ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 2161 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 2221 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 2281 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 2341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 2401 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 2461 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 2521 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 2581 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 2641 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 2701 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2761 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2821 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2881 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2941 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 3001 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 3061 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 3121 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 3181 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 3241 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 3301 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 3361 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 3421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 3481 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 3541 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 3601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 3661 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 3721 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 3781 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 3841 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 3901 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 3961 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 4021 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 4081 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 4141 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 4201 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 4261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 4321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 4381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 4441 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 4501 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 4561 gcgcacattt ccccgaaaag tgccacctga cgtc //