LOCUS BBa_J176125 5431 bp DNA linear 23-May-2024 DEFINITION NLS-pcDNA3.1+ ACCESSION BBa_J176125 VERSION BBa_J176125.1 FEATURES Location/Qualifiers misc_feature 232..819 /label=CMV promoter primer_bind 863..882 /label=T7 promoter priming misc_feature 895..951 /label=MCS primer_bind 1025..1042 /label=BGH reverse priming misc_feature 1031..1255 /label=BGH polyadenylation sequence misc_feature 1301..1729 /label=f1 origin misc_feature 1734..2077 /label=SV40 promoter/ origin misc_feature 3107..3237 /label=SV40 early polyA signal misc_feature 3620..4290 /label=pUC origin misc_feature 4435..5431 /label=AMP resistance gene misc_feature 973..993 /label=NLS - T0100 misc_feature 994..999 /label=X/S mixed site misc_feature 1000..1013 /label=MCS (continued) misc_feature 952..972 /label=BB prefix misc_feature 2139..2933 /label=Neomycin R misc_feature 1..5431 /label=source ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcg 961 gccgcatcta gacccaagaa aaagcgcaag gtaactagag ggcccgttta aacccgctga 1021 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 1081 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 1141 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 1201 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 1261 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 1321 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 1381 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 1441 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 1501 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 1561 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 1621 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 1681 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 1741 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 1801 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 1861 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 1921 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 1981 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 2041 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 2101 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 2161 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 2221 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 2281 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat 2341 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 2401 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 2461 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 2521 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 2581 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 2641 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc 2701 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 2761 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 2821 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 2881 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 2941 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 3001 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 3061 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc 3121 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 3181 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat 3241 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 3301 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 3361 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 3421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 3481 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 3541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 3601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 3901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 4021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 4081 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 4141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 4201 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4321 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 4441 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 4501 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 4561 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 4621 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 4681 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 4741 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 4801 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 4861 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 4921 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 4981 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 5041 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 5101 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 5161 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 5221 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 5281 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 5341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 5401 cacatttccc cgaaaagtgc cacctgacgt c //