LOCUS BBa_J176126 5157 bp DNA linear 11-Oct-2024 DEFINITION V0201 ACCESSION BBa_J176126 VERSION BBa_J176126.1 FEATURES Location/Qualifiers misc_feature 1066..1107 /label=V5 epitope tagging misc_feature 1145..1162 /label=BGH reverse priming site polyA_site 1151..1375 /label=BGH polyadenylation signal misc_feature 1658..1695 /label=FRT site CDS 1713..2705 /label=Hygromycin resistance gene misc_feature 2865..2995 /label=SV40 early polyadelylation signal misc_feature 3378..4051 /label=pUC origin CDS 4196..5056 /label=bla (ampicilin resistance) promoter 5057..5155 /label=bla promoter misc_feature 1020..5157 /label=Digestion product of ApaI and HindIII building bloc variation misc_feature 1..4 /label=Digestion product of ApaI and HindIII building bloc variation misc_feature 1051..1054 /label=digestion overhangs misc_feature 1129..1131 /label=stop codon? misc_feature 1020..1050 /label=micro MCS site for biobrick inclusion misc_feature 1045..1047 /label=SpeI - XbaI ligation misc_feature 1048..1048 /label=SpeI - XbaI ligation primer_bind 906..930 /label=3t3 forward primer bind primer_bind 920..943 /label=3t3 forward primer 2.0 hopefully better misc_feature 1117..1375 /label=possible biobrickable terminator with polyA signal terminator misc_feature 763..767 /label=possible biobrickable terminator with polyA signal terminator misc_feature 331..331 /label=bglobinsulator CDS 65..404 /label=bglobinsulator primer_bind 321..340 /label=bglobinsulator primer_bind 321..340 /label=bglobinsulator primer_bind 390..404 /label=bglobinsulator CDS 411..750 /label=bglobinsulator primer_bind 411..425 /label=bglobinsulator misc_feature 405..410 /label=bglobinsulator misc_feature 677..677 /label=bglobinsulator primer_bind 667..686 /label=bglobinsulator primer_bind 667..686 /label=bglobinsulator primer_bind 736..750 /label=bglobinsulator misc_feature 52..54 /label=bglobinsulator misc_feature 47..51 /label=bglobinsulator misc_feature 760..762 /label=bglobinsulator primer_bind 60..74 /label=bglobinsulator primer_bind 64..64 /label=bglobinsulator misc_feature 1..5157 /label=bglobinsulator ORIGIN 1 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgtacgcatc 61 tagtttaatt aactcgagtc ctaaagaagt cggcttctcc atcggatcct gatcagggga 121 gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct 181 agggggcagc agcgaccgcc cggggctccg ctccggtccg gcgctccccc cgcatcccga 241 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct 301 gaacgcttct cgctgctctt tgagcctgca aacacctggg ggatacgggg aaaatgatca 361 agatcttcta cactgggacg caaggattat cctcgaggcg cgccactaga ttaattaact 421 cgagtcctaa agaagtcggc ttctccatcg gatcctgatc aggggagctc acggggacag 481 ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg 541 accgcccggg gctccgctcc ggtccggcgc tccccccgca tcccgagccg gcagcgtgcg 601 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct 661 gctctttgag cctgcaaaca cctgggggat acggggaaaa tgatcaagat cttctacact 721 gggacgcaag gattatcctc gaggcgcgcc acaagtagct acgatctccc gatcccctat 781 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 841 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 901 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 961 atgtacgggc cagatatacg cgttgacatt gattattgac taggcttttg caaaaagctc 1021 accgcggccg cagtgtcaag ggtactagtc ggcccgcggt tcgaaggtaa gcctatccct 1081 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta aacccgctga 1141 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 1201 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 1261 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 1321 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 1381 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 1441 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 1501 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 1561 caagctctaa atcgggggtc cctttagggt tccgatttag tgctttacgg cacctcgacc 1621 ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct 1681 attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg 1741 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg 1801 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa 1861 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg 1921 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca 1981 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg 2041 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga 2101 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca 2161 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca 2221 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag 2281 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca 2341 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca 2401 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc 2461 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc 2521 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg 2581 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc 2641 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc 2701 gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca 2761 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 2821 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 2881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 2941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 3001 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 3061 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 3121 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 3181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3241 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3301 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3361 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3421 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3481 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3541 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3601 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 3661 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3721 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 3781 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3841 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 3901 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3961 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4021 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4081 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4141 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 4201 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4261 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4321 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4381 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4441 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4501 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 4561 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 4621 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 4681 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 4741 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 4801 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4861 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 4921 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 4981 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 5041 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 5101 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttc //