BBa_J176126 1 BBa_J176126 V0201 2011-10-22T11:00:00Z 2015-08-31T04:08:35Z TBA VP64-NLS-pcDNA3.1+, pcDNA3.1+ with Eco/Spe NLS BioBrick inserted into Eco/Xba; amp, neo false false _863_ 0 10865 9 Not in stock false TBA false Brady Laughlin annotation2160382 1 bla promoter range2160382 1 5057 5155 annotation2160396 1 bglobinsulator range2160396 1 321 340 annotation2160381 1 bla (ampicilin resistance) range2160381 1 4196 5056 annotation2160407 1 bglobinsulator range2160407 1 47 51 annotation2160378 1 Hygromycin resistance gene range2160378 1 1713 2705 annotation2160376 1 BGH polyadenylation signal range2160376 1 1151 1375 annotation2160403 1 bglobinsulator range2160403 1 667 686 annotation2160375 1 BGH reverse priming site range2160375 1 1145 1162 annotation2160411 1 bglobinsulator range2160411 1 1 5157 annotation2160406 1 bglobinsulator range2160406 1 52 54 annotation2160374 1 V5 epitope tagging range2160374 1 1066 1107 annotation2160402 1 bglobinsulator range2160402 1 677 677 annotation2160384 1 Digestion product of ApaI and HindIII building bloc variation range2160384 1 1 4 annotation2160393 1 possible biobrickable terminator with polyA signal terminator range2160393 1 763 767 annotation2160400 1 bglobinsulator range2160400 1 411 425 annotation2160388 1 SpeI - XbaI ligation range2160388 1 1045 1047 annotation2160386 1 stop codon? range2160386 1 1129 1131 annotation2160390 1 3t3 forward primer bind range2160390 1 906 930 annotation2160379 1 SV40 early polyadelylation signal range2160379 1 2865 2995 annotation2160408 1 bglobinsulator range2160408 1 760 762 annotation2160401 1 bglobinsulator range2160401 1 405 410 annotation2160389 1 SpeI - XbaI ligation range2160389 1 1048 1048 annotation2160377 1 FRT site range2160377 1 1658 1695 annotation2160410 1 bglobinsulator range2160410 1 64 64 annotation2160395 1 bglobinsulator range2160395 1 65 404 annotation2160409 1 bglobinsulator range2160409 1 60 74 annotation2160404 1 bglobinsulator range2160404 1 667 686 annotation2160391 1 3t3 forward primer 2.0 hopefully better range2160391 1 920 943 annotation2160399 1 bglobinsulator range2160399 1 411 750 annotation2160394 1 bglobinsulator range2160394 1 331 331 annotation2160398 1 bglobinsulator range2160398 1 390 404 annotation2160397 1 bglobinsulator range2160397 1 321 340 annotation2160380 1 pUC origin range2160380 1 3378 4051 annotation2160387 1 micro MCS site for biobrick inclusion range2160387 1 1020 1050 annotation2160405 1 bglobinsulator range2160405 1 736 750 annotation2160383 1 Digestion product of ApaI and HindIII building bloc variation range2160383 1 1020 5157 annotation2160392 1 possible biobrickable terminator with polyA signal terminator range2160392 1 1117 1375 annotation2160385 1 digestion overhangs range2160385 1 1051 1054 BBa_J176126_sequence 1 cgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcgtacgcatctagtttaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccacaagtagctacgatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactaggcttttgcaaaaagctcaccgcggccgcagtgtcaagggtactagtcggcccgcggttcgaaggtaagcctatccctaaccctctcctcggtctcgattctacgcgtaccggttagtaatgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctagaagttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgccgccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z