##gff-version 3
##sequence-region BBa_J176126 1 5157
BBa_J176126	.	CDS	1713	2705	.	+	0	ID=annotation2160378;Name=Hygromycin resistance gene
BBa_J176126	.	CDS	4196	5056	.	+	0	ID=annotation2160381;Name=bla (ampicilin resistance)
BBa_J176126	.	sequence_feature	1020	5157	.	+	0	ID=annotation2160383;Name=Digestion product of ApaI and HindIII building bloc	variation
BBa_J176126	.	polyA_site	1151	1375	.	+	0	ID=annotation2160376;Name=BGH polyadenylation signal
BBa_J176126	.	sequence_feature	1145	1162	.	+	0	ID=annotation2160375;Name=BGH reverse priming site
BBa_J176126	.	primer_binding_site	667	686	.	+	0	ID=annotation2160403;Name=bglobinsulator
BBa_J176126	.	primer_binding_site	736	750	.	+	0	ID=annotation2160405;Name=bglobinsulator
BBa_J176126	.	primer_binding_site	411	425	.	+	0	ID=annotation2160400;Name=bglobinsulator
BBa_J176126	.	sequence_feature	677	677	.	+	0	ID=annotation2160402;Name=bglobinsulator
BBa_J176126	.	sequence_feature	1	5157	.	+	0	ID=annotation2160411;Name=bglobinsulator
BBa_J176126	.	sequence_feature	1045	1047	.	+	0	ID=annotation2160388;Name=SpeI - XbaI ligation
BBa_J176126	.	sequence_feature	2865	2995	.	+	0	ID=annotation2160379;Name=SV40 early polyadelylation signal
BBa_J176126	.	primer_binding_site	906	930	.	+	0	ID=annotation2160390;Name=3t3 forward primer bind
BBa_J176126	.	sequence_feature	760	762	.	+	0	ID=annotation2160408;Name=bglobinsulator
BBa_J176126	.	sequence_feature	1129	1131	.	+	0	ID=annotation2160386;Name=stop codon?
BBa_J176126	.	CDS	65	404	.	+	0	ID=annotation2160395;Name=bglobinsulator
BBa_J176126	.	sequence_feature	405	410	.	+	0	ID=annotation2160401;Name=bglobinsulator
BBa_J176126	.	promoter	5057	5155	.	+	0	ID=annotation2160382;Name=bla promoter
BBa_J176126	.	primer_binding_site	60	74	.	+	0	ID=annotation2160409;Name=bglobinsulator
BBa_J176126	.	sequence_feature	1020	1050	.	+	0	ID=annotation2160387;Name=micro MCS site for biobrick inclusion
BBa_J176126	.	sequence_feature	763	767	.	+	0	ID=annotation2160393;Name=possible biobrickable terminator with polyA signal	terminator
BBa_J176126	.	sequence_feature	47	51	.	+	0	ID=annotation2160407;Name=bglobinsulator
BBa_J176126	.	sequence_feature	1	4	.	+	0	ID=annotation2160384;Name=Digestion product of ApaI and HindIII building bloc	variation
BBa_J176126	.	primer_binding_site	390	404	.	+	0	ID=annotation2160398;Name=bglobinsulator
BBa_J176126	.	sequence_feature	1048	1048	.	+	0	ID=annotation2160389;Name=SpeI - XbaI ligation
BBa_J176126	.	CDS	411	750	.	+	0	ID=annotation2160399;Name=bglobinsulator
BBa_J176126	.	primer_binding_site	321	340	.	+	0	ID=annotation2160397;Name=bglobinsulator
BBa_J176126	.	primer_binding_site	920	943	.	+	0	ID=annotation2160391;Name=3t3 forward primer 2.0 hopefully better
BBa_J176126	.	sequence_feature	52	54	.	+	0	ID=annotation2160406;Name=bglobinsulator
BBa_J176126	.	sequence_feature	3378	4051	.	+	0	ID=annotation2160380;Name=pUC origin
BBa_J176126	.	primer_binding_site	667	686	.	+	0	ID=annotation2160404;Name=bglobinsulator
BBa_J176126	.	primer_binding_site	321	340	.	+	0	ID=annotation2160396;Name=bglobinsulator
BBa_J176126	.	primer_binding_site	64	64	.	+	0	ID=annotation2160410;Name=bglobinsulator
BBa_J176126	.	sequence_feature	1066	1107	.	+	0	ID=annotation2160374;Name=V5 epitope tagging
BBa_J176126	.	sequence_feature	331	331	.	+	0	ID=annotation2160394;Name=bglobinsulator
BBa_J176126	.	sequence_feature	1658	1695	.	+	0	ID=annotation2160377;Name=FRT site
BBa_J176126	.	sequence_feature	1117	1375	.	+	0	ID=annotation2160392;Name=possible biobrickable terminator with polyA signal	terminator
BBa_J176126	.	sequence_feature	1051	1054	.	+	0	ID=annotation2160385;Name=digestion overhangs
>BBa_J176126
cgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatcgtacgcatctagtttaattaactcgagt
cctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggat
gtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcc
cgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgc
tctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcg
aggcgcgccactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacgg
ggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggct
ccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgg
gatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatc
ttctacactgggacgcaaggattatcctcgaggcgcgccacaagtagctacgatctcccgatcccctatggtgcactct
cagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgc
gcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttg
cgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactaggcttttgcaaaaagctcaccgcgg
ccgcagtgtcaagggtactagtcggcccgcggttcgaaggtaagcctatccctaaccctctcctcggtctcgattctac
gcgtaccggttagtaatgagtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgc
ccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgc
attgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatag
caggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagctggggctctagggggtatccccac
gcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctag
cgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggtc
cctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtacctaga
agttcctattccgaagttcctattctctagaaagtataggaacttccttggccaaaaagcctgaactcaccgcgacgtc
tgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgct
ttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatg
tttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgaccta
ttgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtc
gcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcg
gtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacga
caccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctc
gtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcga
tgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcg
ctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaa
ctctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccg
gagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgc
cgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatagcacgtactacgagatttcgattccaccgcc
gccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctccagcgcggggatctcatgc
tggagttcttcgcccaccccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcac
aaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccg
tcgacctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccac
acaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcg
ctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggt
ttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag
ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagca
aaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat
cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc
gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatag
ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagccc
gaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca
ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacac
tagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggc
aaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaag
atcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatc
aaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttgg
tctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgac
tccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacg
ctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatcc
gcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttg
ccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcg
agttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggcc
gcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtga
ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacggga
taataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatc
ttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcg
tttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat
actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttag
aaaaataaacaaataggggttc