LOCUS BBa_J176127 4968 bp DNA linear 22-Nov-2024 DEFINITION V0120 ACCESSION BBa_J176127 VERSION BBa_J176127.1 FEATURES Location/Qualifiers primer_bind 1..20 /label=V0120 forward primer misc_feature 101..121 /label=EcoRI NotI XbaI misc_feature 122..245 /label=attR1 CDS 355..1013 /label=Cm(R) CDS 1357..1661 /label=ccdB misc_feature 1702..1824 /label=attR2 misc_feature 1825..1844 /label=SpeI NotI PstI primer_bind 1940..1962 /label=V0120 reverse primer CDS 3079..3936 /label=Ampicillin resistance promoter 3937..4035 /label=bla ORIGIN 1 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattggaggc tacagtcagt 61 ggagaggact ttcactgact gactgactgc gtctcaacct gaattcgcgg ccgcatctag 121 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 181 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 241 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat 301 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag 361 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 421 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 481 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 541 cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg 601 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 661 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 721 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 781 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 841 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 901 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc 961 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct 1021 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 1081 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc 1141 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 1201 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 1261 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 1321 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata 1381 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 1441 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 1501 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 1561 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 1621 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac 1681 acagccagtc ttcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 1741 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 1801 cgttcagctt tctacaaagt ggtgactagt agcggccgct gcagagcaag agacgactga 1861 ctgactgact ggaaagagga aggctggaag aggaaggagc ttggcgtaat catggtcata 1921 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 1981 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 2041 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 2101 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 2161 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 2221 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 2281 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 2341 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 2401 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 2461 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 2521 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 2581 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 2641 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 2701 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 2761 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 2821 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 2881 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 2941 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 3001 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 3061 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 3121 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 3181 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 3241 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 3301 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 3361 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 3421 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 3481 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3541 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 3601 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 3661 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 3721 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 3781 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3841 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 3901 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 3961 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 4021 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 4081 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 4141 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 4201 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 4261 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 4321 taaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 4381 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga 4441 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 4501 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 4561 caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 4621 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 4681 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 4741 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac tatggttgct ttgacgtatg 4801 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca 4861 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 4921 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgcca //