LOCUS BBa_J176128 4742 bp DNA linear 23-Dec-2024 DEFINITION pcDNA3.1+ neo ACCESSION BBa_J176128 VERSION BBa_J176128.1 FEATURES Location/Qualifiers misc_feature 232..249 /label=CMV promoter remnant misc_feature 250..324 /label=SpeI EcoRI NotI XbaI primer_bind 336..353 /label=BGH polyA_site 342..566 /label=BGH misc_feature 612..1040 /label=f1 origin promoter 1045..1388 /label=SV40 CDS 1450..2244 /label=Neomycin resistance polyA_site 2418..2548 /label=SV40 misc_feature 2931..3601 /label=pUC origin CDS 3746..4606 /label=Ampicillin resistance promoter 4607..4705 /label=bla primer_bind 186..203 /label=DD122B ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct 301 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 361 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 421 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 481 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 541 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag 601 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 661 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 841 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 901 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 961 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 1021 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc 1081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 1141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 1201 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 1261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 1321 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 1381 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga 1441 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 1501 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 1561 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 1621 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 1681 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 1741 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 1801 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 1861 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 1921 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 1981 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 2041 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 2101 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 2161 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 2221 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 2281 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 2341 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 2401 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2461 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2521 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 2581 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 2641 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 2701 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 2761 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 2821 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 2881 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 2941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3301 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3421 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca 3541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 3601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 3661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 3721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 3781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 3841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 3901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 3961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 4021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 4081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 4141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 4201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 4261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 4321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 4381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 4441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 4501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 4561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 4621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 4681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 4741 tc //