LOCUS BBa_J176129 5587 bp DNA linear 23-Dec-2024 DEFINITION pcVN ACCESSION BBa_J176129 VERSION BBa_J176129.1 FEATURES Location/Qualifiers misc_feature 232..819 /label=CMV promoter primer_bind 863..882 /label=T7 promoter priming misc_feature 895..951 /label=MCS primer_bind 1181..1198 /label=BGH reverse priming misc_feature 1187..1411 /label=BGH polyadenylation sequence misc_feature 1457..1885 /label=f1 origin misc_feature 1890..2233 /label=SV40 promoter/origin misc_feature 3263..3393 /label=SV40 early polyA signal misc_feature 3776..4446 /label=pUC origin misc_feature 4591..5587 /label=Amp resistance gene misc_feature 1129..1149 /label=NLS - T0100 misc_feature 1150..1155 /label=X/S mixed site misc_feature 1156..1169 /label=MCS (continued misc_feature 952..972 /label=BB prefix misc_feature 2295..3089 /label=Neomycin R misc_feature 973..1122 /label=VP64 misc_feature 1123..1128 /label=X/S mixed site misc_feature 1..5587 /label=source ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcg 961 gccgcatcta gagacgcttt ggacgacttc gacttggaca tgttgggttc tgacgctttg 1021 gacgacttcg acttggacat gttgggttct gacgctttgg acgacttcga cttggacatg 1081 ttgggttctg acgctttgga cgacttcgac ttggacatgt tgactagacc caagaaaaag 1141 cgcaaggtaa ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt 1201 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 1261 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 1321 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 1381 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 1441 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 1501 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 1561 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 1621 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 1681 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 1741 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 1801 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 1861 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa 1921 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 1981 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 2041 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 2101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 2161 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 2221 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 2281 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 2341 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 2401 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 2461 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 2521 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 2581 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 2641 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 2701 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 2761 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 2821 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 2881 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 2941 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 3001 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 3061 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 3121 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 3181 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 3241 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 3301 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 3361 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 3421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 3481 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 3541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 3601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 3661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4261 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt 4381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 4441 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 4501 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 4561 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 4621 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 4681 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 4741 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 4801 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 4861 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 4921 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 4981 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 5041 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 5101 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 5161 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 5221 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 5281 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 5341 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 5401 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 5461 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 5521 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 5581 tgacgtc //