LOCUS BBa_J176136 3503 bp DNA linear 22-Nov-2024 DEFINITION HPK-RFP-miRts BCL2 ACCESSION BBa_J176136 VERSION BBa_J176136.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 RBS 523..540 /label=BBa_J176012 misc_feature 541..546 /label=BBa_J176041 misc_feature 547..1005 /label=BBa_J176020 misc_feature 1006..1011 /label=BBa_J176041 misc_feature 1012..1545 /label=BBa_J176018 misc_feature 1546..1551 /label=BBa_J176041 misc_feature 1552..2256 /label=BBa_J176005 misc_feature 2257..2262 /label=BBa_J176041 misc_feature 2263..2967 /label=BBa_J176005 misc_feature 2968..2973 /label=BBa_J176041 misc_feature 2974..2994 /label=BBa_J176008 misc_feature 2995..3000 /label=BBa_J176041 terminator 3001..3013 /label=BBa_J176016 misc_feature 3014..3019 /label=BBa_J176041 misc_feature 3020..3039 /label=Bcl2 miR-34 target misc_feature 3040..3059 /label=Bcl2 miR-34 target misc_feature 3060..3079 /label=Bcl2 miR-34 target misc_feature 3020..3079 /label=BBa_J176133 misc_feature 3080..3085 /label=BBa_J176041 misc_feature 3113..3131 /label=repeat 1 misc_feature 3140..3158 /label=repeat 2 misc_feature 3167..3185 /label=repeat 3 misc_feature 3194..3212 /label=repeat 4 misc_feature 3220..3238 /label=repeat 5 misc_feature 3086..3272 /label=BBa_J176025 misc_feature 3273..3278 /label=BBa_J176041 misc_feature 3279..3503 /label=BBa_J176009 misc_feature 3086..3503 /label=BBa_S04730 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacccgccgc caccatggag 541 actagaatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag 601 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt 661 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa 721 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac 781 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta 841 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg 901 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac 961 aaaggtcaaa gacagttgac tgtatcgccg gaatttccgg ggatcactag agcatccaac 1021 ttgaacattt cgagaaagct taccatatca accccatcat gctctttcga aaattcaaat 1081 agcacatcca ttccttcgcc cgcttcctca tctcaaagcc acactccaat gagaaacatg 1141 agctcactct ctgataacag cgttttcagc cggaatatgg aacaatcatc accaatcact 1201 ccaagtatgt accaatttgg tcagcagcag tcaaacagta tatgtggtag caccgttagt 1261 gtgaatagtc tggtgaatac aaataacaaa caaaggatct acgaacaaat cacgggtcct 1321 aacagcaata acgcaaccaa tgattatatt gatttgctaa acctaaatga gtctaacaag 1381 gaaaaccaaa atcccgcaac ggcgcattac ctcaatgggg gcccacccaa gacaagcttc 1441 attaaccatg gaatgttccc ctcgccaact gggaccataa atagcggtaa atctagcagt 1501 gcctcatctt taatttcttt tggtatgggc aatacccaag taataactag agtgagcaag 1561 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 1621 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 1681 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 1741 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 1801 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 1861 gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc 1921 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 1981 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 2041 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 2101 acctacaagg ccaagaagcc cgtgcagcta cccggcgcct acaacgtcaa catcaagttg 2161 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 2221 cgccactcca ccggcggcat ggacgagctg tacaagacta gagtgagcaa gggcgaggag 2281 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg 2341 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 2401 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc 2461 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac 2521 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 2581 ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg 2641 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc 2701 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag 2761 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag 2821 gccaagaagc ccgtgcagct acccggcgcc tacaacgtca acatcaagtt ggacatcacc 2881 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc 2941 accggcggca tggacgagct gtacaagact agacccaaga aaaagcgcaa ggtaactaga 3001 gcgtaaagct gagactagag aatcagctat ttactgccag aatcagctat ttactgccag 3061 aatcagctat ttactgccaa ctagaatagt tgtttaaacg agctcgctag caaatcaaga 3121 gtcctcataa aggaattaaa atcaagagtc ctcataaagt ctagcaaatc aagagtcctc 3181 ataaagctag acaaatcaag agtcctcata aataattcca atcaagagtc ctcataaagt 3241 cgacctgcaa gcatgcaagc tgatccggct gcactagact gtgccttcta gttgccagcc 3301 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 3361 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 3421 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 3481 tggggatgcg gtgggctcta tgg //