BBa_J176009 1 polyA polyA2 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA BGH polyA signal cloned from pcDNA5/FRT vector false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176136 1 RFP-miRtsB HPK-RFP-miRts BCL2 2011-12-10T12:00:00Z 2015-08-31T04:08:35Z TBA TBA false false _863_ 0 1144 61 Not in stock false TBA false Karmella Haynes, Daniel Flicker component2167416 1 BBa_J176041 component2167409 1 BBa_J176012 component2167420 1 BBa_J176041 component2167408 1 BBa_J176041 component2167407 1 BBa_J176028 component2167426 1 BBa_J176133 component2167412 1 BBa_J176041 component2167427 1 BBa_J176041 component2167415 1 BBa_J176005 component2167417 1 BBa_J176005 component2167419 1 BBa_J176008 component2167421 1 BBa_J176016 component2167410 1 BBa_J176041 component2167418 1 BBa_J176041 component2167422 1 BBa_J176041 component2167411 1 BBa_J176020 component2167414 1 BBa_J176041 component2167431 1 BBa_S04730 component2167413 1 BBa_J176018 annotation2167415 1 BBa_J176005 range2167415 1 1552 2256 annotation2167413 1 BBa_J176018 range2167413 1 1012 1545 annotation2167408 1 BBa_J176041 range2167408 1 517 522 annotation2167410 1 BBa_J176041 range2167410 1 541 546 annotation2167412 1 BBa_J176041 range2167412 1 1006 1011 annotation2167426 1 BBa_J176133 range2167426 1 3020 3079 annotation2167414 1 BBa_J176041 range2167414 1 1546 1551 annotation2167409 1 BBa_J176012 range2167409 1 523 540 annotation2167416 1 BBa_J176041 range2167416 1 2257 2262 annotation2167421 1 BBa_J176016 range2167421 1 3001 3013 annotation2167427 1 BBa_J176041 range2167427 1 3080 3085 annotation2167407 1 BBa_J176028 range2167407 1 1 516 annotation2167431 1 BBa_S04730 range2167431 1 3086 3503 annotation2167417 1 BBa_J176005 range2167417 1 2263 2967 annotation2167420 1 BBa_J176041 range2167420 1 2995 3000 annotation2167411 1 BBa_J176020 range2167411 1 547 1005 annotation2167418 1 BBa_J176041 range2167418 1 2968 2973 annotation2167419 1 BBa_J176008 range2167419 1 2974 2994 annotation2167422 1 BBa_J176041 range2167422 1 3014 3019 BBa_J176016 1 STOP STOP 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA contains stop codons TAA and TGA in frame false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176028 1 HPK HPK 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166554 1 mutated PstI range2166554 1 511 511 annotation2166553 1 mutated SpeI range2166553 1 253 253 BBa_S04730 1 BBa_S04730 J176025:J176009 2011-10-14T11:00:00Z 2015-05-08T01:14:44Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2158521 1 BBa_J176025 component2158522 1 BBa_J176041 component2158523 1 BBa_J176009 annotation2158522 1 BBa_J176041 range2158522 1 188 193 annotation2158521 1 BBa_J176025 range2158521 1 1 187 annotation2158523 1 BBa_J176009 range2158523 1 194 418 BBa_J176133 1 miRts BCL2 miRts BCL2 2011-12-10T12:00:00Z 2015-08-31T04:08:35Z Synthesized de novo (IDT DNA) Insert this sequence into the 3' UTR of your gene construct (i.e., after the ORF) to place it under control of the natural microRNA miR-34 in human cells. false false _863_ 0 1144 61 Not in stock false This sequence contains three short repeats. false Karmella Haynes, Francesca Ceroni annotation2166748 1 Bcl2 miR-34 target range2166748 1 21 40 annotation2166747 1 Bcl2 miR-34 target range2166747 1 1 20 annotation2166749 1 Bcl2 miR-34 target range2166749 1 41 60 BBa_J176012 1 K Kozak 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 yeast/ human Kozak plus start codon false false _863_ 0 10865 9 In stock false TBA false Brady Laughlin BBa_J176041 1 scar Silver scar 2011-10-04T11:00:00Z 2015-08-31T04:08:34Z This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard). Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard). false false _863_ 0 1144 61 Not in stock false The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts. false Karmella Haynes BBa_J176020 1 Gal4DB Gal4 DB 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Gal4 DNA binding domain (remade by KAH) false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176005 1 mCh mCh 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Humanized mCherry fluorescent protein false false _863_ 0 10865 9 It's complicated In stock false TBA false Karmella Haynes BBa_J176018 1 PEST PEST 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA degradation tag false false _863_ 0 10865 9 It's complicated false TBA false Karmella Haynes BBa_J176008 1 NLS NLS 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 SV40 nuclear localization sequence false false _863_ 0 10865 9 In stock false TBA false Karmella Haynes BBa_J176025 1 miRts luc miRts luc 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA miRNA target for luciferase RNAi false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166811 1 repeat 5 range2166811 1 135 153 annotation2166809 1 repeat 3 range2166809 1 82 100 annotation2166807 1 repeat 1 range2166807 1 28 46 annotation2166808 1 repeat 2 range2166808 1 55 73 annotation2166810 1 repeat 4 range2166810 1 109 127 BBa_J176133_sequence 1 gaatcagctatttactgccagaatcagctatttactgccagaatcagctatttactgcca BBa_J176136_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacccgccgccaccatggagactagaatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagactagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagagaatcagctatttactgccagaatcagctatttactgccagaatcagctatttactgccaactagaatagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176018_sequence 1 gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata BBa_J176005_sequence 1 gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag BBa_J176041_sequence 1 actaga BBa_J176009_sequence 1 ctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176020_sequence 1 atgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatc BBa_J176016_sequence 1 gcgtaaagctgag BBa_J176012_sequence 1 cccgccgccaccatggag BBa_J176025_sequence 1 atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgc BBa_S04730_sequence 1 atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176028_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc BBa_J176008_sequence 1 cccaagaaaaagcgcaaggta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z