BBa_J176009 1 polyA polyA2 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA BGH polyA signal cloned from pcDNA5/FRT vector false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176016 1 STOP STOP 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA contains stop codons TAA and TGA in frame false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176028 1 HPK HPK 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166554 1 mutated PstI range2166554 1 511 511 annotation2166553 1 mutated SpeI range2166553 1 253 253 BBa_J176134 1 miRts CCND miRts CCND1 2011-12-10T12:00:00Z 2015-08-31T04:08:35Z De novo DNA synthesis (IDT DNA) TBA false false _863_ 0 1144 61 Not in stock false Contains three short repeats false Karmella Haynes, Francesca Ceroni annotation2166750 1 CCND1 miR-34 target range2166750 1 1 20 annotation2166752 1 CCND1 miR-34 target range2166752 1 41 60 annotation2166751 1 CCND1 miR-34 target range2166751 1 21 40 BBa_S04730 1 BBa_S04730 J176025:J176009 2011-10-14T11:00:00Z 2015-05-08T01:14:44Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2158521 1 BBa_J176025 component2158522 1 BBa_J176041 component2158523 1 BBa_J176009 annotation2158522 1 BBa_J176041 range2158522 1 188 193 annotation2158521 1 BBa_J176025 range2158521 1 1 187 annotation2158523 1 BBa_J176009 range2158523 1 194 418 BBa_J176012 1 K Kozak 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 yeast/ human Kozak plus start codon false false _863_ 0 10865 9 In stock false TBA false Brady Laughlin BBa_J176041 1 scar Silver scar 2011-10-04T11:00:00Z 2015-08-31T04:08:34Z This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard). Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard). false false _863_ 0 1144 61 Not in stock false The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts. false Karmella Haynes BBa_J176137 1 RFP-miRtsC HPK-RFP-miRts CCND1 2011-12-10T12:00:00Z 2015-08-31T04:08:35Z TBA TBA false false _863_ 0 1144 61 Not in stock false TBA false Karmella Haynes, Daniel Flicker component2238491 1 BBa_J176008 component2238480 1 BBa_J176039 component2238498 1 BBa_J176134 component2238488 1 BBa_J176039 component2238489 1 BBa_J176005 component2238485 1 BBa_J176018 component2238492 1 BBa_J176039 component2238483 1 BBa_J176020 component2238494 1 BBa_J176039 component2238486 1 BBa_J176039 component2238503 1 BBa_S04730 component2238481 1 BBa_J176012 component2238484 1 BBa_J176039 component2238490 1 BBa_J176039 component2238482 1 BBa_J176039 component2238487 1 BBa_J176005 component2238499 1 BBa_J176039 component2238479 1 BBa_J176028 component2238493 1 BBa_J176016 annotation2238490 1 BBa_J176039 range2238490 1 3148 3189 annotation2238482 1 BBa_J176039 range2238482 1 577 618 annotation2238493 1 BBa_J176016 range2238493 1 3253 3265 annotation2238485 1 BBa_J176018 range2238485 1 1120 1653 annotation2238479 1 BBa_J176028 range2238479 1 1 516 annotation2238491 1 BBa_J176008 range2238491 1 3190 3210 annotation2238498 1 BBa_J176134 range2238498 1 3308 3367 annotation2238487 1 BBa_J176005 range2238487 1 1696 2400 annotation2238499 1 BBa_J176039 range2238499 1 3368 3409 annotation2238503 1 BBa_S04730 range2238503 1 3410 3827 annotation2238492 1 BBa_J176039 range2238492 1 3211 3252 annotation2238494 1 BBa_J176039 range2238494 1 3266 3307 annotation2238481 1 BBa_J176012 range2238481 1 559 576 annotation2238484 1 BBa_J176039 range2238484 1 1078 1119 annotation2238489 1 BBa_J176005 range2238489 1 2443 3147 annotation2238486 1 BBa_J176039 range2238486 1 1654 1695 annotation2238483 1 BBa_J176020 range2238483 1 619 1077 annotation2238488 1 BBa_J176039 range2238488 1 2401 2442 annotation2238480 1 BBa_J176039 range2238480 1 517 558 BBa_J176020 1 Gal4DB Gal4 DB 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Gal4 DNA binding domain (remade by KAH) false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176039 1 Spacer Spacer 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Noncoding spacer to put between TF binding site and TATA false false _863_ 0 10865 9 Not in stock false TBA false Brady Laughlin, Karmella Haynes BBa_J176005 1 mCh mCh 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Humanized mCherry fluorescent protein false false _863_ 0 10865 9 It's complicated In stock false TBA false Karmella Haynes BBa_J176018 1 PEST PEST 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA degradation tag false false _863_ 0 10865 9 It's complicated false TBA false Karmella Haynes BBa_J176008 1 NLS NLS 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 SV40 nuclear localization sequence false false _863_ 0 10865 9 In stock false TBA false Karmella Haynes BBa_J176025 1 miRts luc miRts luc 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA miRNA target for luciferase RNAi false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166811 1 repeat 5 range2166811 1 135 153 annotation2166809 1 repeat 3 range2166809 1 82 100 annotation2166807 1 repeat 1 range2166807 1 28 46 annotation2166808 1 repeat 2 range2166808 1 55 73 annotation2166810 1 repeat 4 range2166810 1 109 127 BBa_J176134_sequence 1 acaatgtcatatactgccatacaatgtcatatactgccatacaatgtcatatactgccat BBa_J176018_sequence 1 gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata BBa_J176005_sequence 1 gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag BBa_J176041_sequence 1 actaga BBa_J176009_sequence 1 ctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176020_sequence 1 atgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatc BBa_J176016_sequence 1 gcgtaaagctgag BBa_J176012_sequence 1 cccgccgccaccatggag BBa_J176039_sequence 1 gtgacgccggtgacgccgactagagtgacgccggtgacgccg BBa_J176025_sequence 1 atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgc BBa_S04730_sequence 1 atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176028_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc BBa_J176137_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccgtgacgccggtgacgccgactagagtgacgccggtgacgccgcccgccgccaccatggaggtgacgccggtgacgccgactagagtgacgccggtgacgccgatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcgtgacgccggtgacgccgactagagtgacgccggtgacgccggcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaatagtgacgccggtgacgccgactagagtgacgccggtgacgccggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggtgacgccggtgacgccgactagagtgacgccggtgacgccggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggtgacgccggtgacgccgactagagtgacgccggtgacgccgcccaagaaaaagcgcaaggtagtgacgccggtgacgccgactagagtgacgccggtgacgccggcgtaaagctgaggtgacgccggtgacgccgactagagtgacgccggtgacgccgacaatgtcatatactgccatacaatgtcatatactgccatacaatgtcatatactgccatgtgacgccggtgacgccgactagagtgacgccggtgacgccgatagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176008_sequence 1 cccaagaaaaagcgcaaggta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z