RFP-miRtsC
type: ComponentDefinition
persistent identity: BBa J176137
display id: BBa_J176137
version: 1
was generated by: iGEM to SBOL conversion
description: HPK-RFP-miRts CCND1
created: 2011-12-10T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes, Daniel Flicker
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J176039
component: BBa_S04730
component: BBa_J176005
component: BBa_J176008
component: BBa_J176016
component: BBa_J176028
component: BBa_J176012
component: BBa_J176020
component: BBa_J176018
component: BBa_J176134
sequence annotation: BBa_J176028
sequence annotation: BBa_J176039
sequence annotation: BBa_J176012
sequence annotation: BBa_J176020
sequence annotation: BBa_J176018
sequence annotation: BBa_J176005
sequence annotation: BBa_J176008
sequence annotation: BBa_J176016
sequence annotation: BBa_J176134
sequence annotation: BBa_S04730
sequence: https://synbiohub.org/public/igem/BBa_J176137_sequence/1

BBa_J176039
type: Component
persistent identity: component2238494
display id: component2238494
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_S04730
type: Component
persistent identity: component2238503
display id: component2238503
version: 1
top level: RFP-miRtsC
definition: BBa_S04730
access: public

BBa_J176039
type: Component
persistent identity: component2238499
display id: component2238499
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_J176005
type: Component
persistent identity: component2238487
display id: component2238487
version: 1
top level: RFP-miRtsC
definition: mCh
access: public

BBa_J176039
type: Component
persistent identity: component2238486
display id: component2238486
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_J176005
type: Component
persistent identity: component2238489
display id: component2238489
version: 1
top level: RFP-miRtsC
definition: mCh
access: public

BBa_J176039
type: Component
persistent identity: component2238488
display id: component2238488
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_J176008
type: Component
persistent identity: component2238491
display id: component2238491
version: 1
top level: RFP-miRtsC
definition: NLS
access: public

BBa_J176039
type: Component
persistent identity: component2238490
display id: component2238490
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_J176016
type: Component
persistent identity: component2238493
display id: component2238493
version: 1
top level: RFP-miRtsC
definition: STOP
access: public

BBa_J176039
type: Component
persistent identity: component2238492
display id: component2238492
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_J176028
type: Component
persistent identity: component2238479
display id: component2238479
version: 1
top level: RFP-miRtsC
definition: HPK
access: public

BBa_J176012
type: Component
persistent identity: component2238481
display id: component2238481
version: 1
top level: RFP-miRtsC
definition: K
access: public

BBa_J176039
type: Component
persistent identity: component2238480
display id: component2238480
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_J176020
type: Component
persistent identity: component2238483
display id: component2238483
version: 1
top level: RFP-miRtsC
definition: Gal4DB
access: public

BBa_J176039
type: Component
persistent identity: component2238482
display id: component2238482
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_J176018
type: Component
persistent identity: component2238485
display id: component2238485
version: 1
top level: RFP-miRtsC
definition: PEST
access: public

BBa_J176039
type: Component
persistent identity: component2238484
display id: component2238484
version: 1
top level: RFP-miRtsC
definition: Spacer
access: public

BBa_J176134
type: Component
persistent identity: component2238498
display id: component2238498
version: 1
top level: RFP-miRtsC
definition: miRts CCND
access: public

BBa_J176028
type: SequenceAnnotation
persistent identity: annotation2238479
display id: annotation2238479
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238479/range2238479/1
component: BBa_J176028

https://synbiohub.org/public/igem/BBa_J176137/annotation2238479/range2238479/1
type: Range
persistent identity: range2238479
display id: range2238479
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 1
end: 516
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238480
display id: annotation2238480
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238480/range2238480/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238480/range2238480/1
type: Range
persistent identity: range2238480
display id: range2238480
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 517
end: 558
orientation: inline

BBa_J176012
type: SequenceAnnotation
persistent identity: annotation2238481
display id: annotation2238481
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238481/range2238481/1
component: BBa_J176012

https://synbiohub.org/public/igem/BBa_J176137/annotation2238481/range2238481/1
type: Range
persistent identity: range2238481
display id: range2238481
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 559
end: 576
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238482
display id: annotation2238482
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238482/range2238482/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238482/range2238482/1
type: Range
persistent identity: range2238482
display id: range2238482
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 577
end: 618
orientation: inline

BBa_J176020
type: SequenceAnnotation
persistent identity: annotation2238483
display id: annotation2238483
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238483/range2238483/1
component: BBa_J176020

https://synbiohub.org/public/igem/BBa_J176137/annotation2238483/range2238483/1
type: Range
persistent identity: range2238483
display id: range2238483
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 619
end: 1077
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238484
display id: annotation2238484
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238484/range2238484/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238484/range2238484/1
type: Range
persistent identity: range2238484
display id: range2238484
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 1078
end: 1119
orientation: inline

BBa_J176018
type: SequenceAnnotation
persistent identity: annotation2238485
display id: annotation2238485
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238485/range2238485/1
component: BBa_J176018

https://synbiohub.org/public/igem/BBa_J176137/annotation2238485/range2238485/1
type: Range
persistent identity: range2238485
display id: range2238485
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 1120
end: 1653
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238486
display id: annotation2238486
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238486/range2238486/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238486/range2238486/1
type: Range
persistent identity: range2238486
display id: range2238486
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 1654
end: 1695
orientation: inline

BBa_J176005
type: SequenceAnnotation
persistent identity: annotation2238487
display id: annotation2238487
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238487/range2238487/1
component: BBa_J176005

https://synbiohub.org/public/igem/BBa_J176137/annotation2238487/range2238487/1
type: Range
persistent identity: range2238487
display id: range2238487
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 1696
end: 2400
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238488
display id: annotation2238488
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238488/range2238488/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238488/range2238488/1
type: Range
persistent identity: range2238488
display id: range2238488
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 2401
end: 2442
orientation: inline

BBa_J176005
type: SequenceAnnotation
persistent identity: annotation2238489
display id: annotation2238489
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238489/range2238489/1
component: BBa_J176005

https://synbiohub.org/public/igem/BBa_J176137/annotation2238489/range2238489/1
type: Range
persistent identity: range2238489
display id: range2238489
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 2443
end: 3147
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238490
display id: annotation2238490
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238490/range2238490/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238490/range2238490/1
type: Range
persistent identity: range2238490
display id: range2238490
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 3148
end: 3189
orientation: inline

BBa_J176008
type: SequenceAnnotation
persistent identity: annotation2238491
display id: annotation2238491
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238491/range2238491/1
component: BBa_J176008

https://synbiohub.org/public/igem/BBa_J176137/annotation2238491/range2238491/1
type: Range
persistent identity: range2238491
display id: range2238491
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 3190
end: 3210
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238492
display id: annotation2238492
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238492/range2238492/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238492/range2238492/1
type: Range
persistent identity: range2238492
display id: range2238492
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 3211
end: 3252
orientation: inline

BBa_J176016
type: SequenceAnnotation
persistent identity: annotation2238493
display id: annotation2238493
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238493/range2238493/1
component: BBa_J176016

https://synbiohub.org/public/igem/BBa_J176137/annotation2238493/range2238493/1
type: Range
persistent identity: range2238493
display id: range2238493
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 3253
end: 3265
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238494
display id: annotation2238494
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238494/range2238494/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238494/range2238494/1
type: Range
persistent identity: range2238494
display id: range2238494
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 3266
end: 3307
orientation: inline

BBa_J176134
type: SequenceAnnotation
persistent identity: annotation2238498
display id: annotation2238498
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238498/range2238498/1
component: BBa_J176134

https://synbiohub.org/public/igem/BBa_J176137/annotation2238498/range2238498/1
type: Range
persistent identity: range2238498
display id: range2238498
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 3308
end: 3367
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238499
display id: annotation2238499
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238499/range2238499/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176137/annotation2238499/range2238499/1
type: Range
persistent identity: range2238499
display id: range2238499
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 3368
end: 3409
orientation: inline

BBa_S04730
type: SequenceAnnotation
persistent identity: annotation2238503
display id: annotation2238503
version: 1
top level: RFP-miRtsC
location: https://synbiohub.org/public/igem/BBa_J176137/annotation2238503/range2238503/1
component: BBa_S04730

https://synbiohub.org/public/igem/BBa_J176137/annotation2238503/range2238503/1
type: Range
persistent identity: range2238503
display id: range2238503
version: 1
top level: RFP-miRtsC
direction: direction/reverse
start: 3410
end: 3827
orientation: inline

mCh
type: ComponentDefinition
persistent identity: BBa J176005
display id: BBa_J176005
version: 1
was generated by: iGEM to SBOL conversion
description: mCh
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Humanized mCherry fluorescent protein
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
sample status: In stock
status: status/Available
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176005_sequence/1

NLS
type: ComponentDefinition
persistent identity: BBa J176008
display id: BBa_J176008
version: 1
was generated by: iGEM to SBOL conversion
description: NLS
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: SV40 nuclear localization sequence
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176008_sequence/1

K
type: ComponentDefinition
persistent identity: BBa J176012
display id: BBa_J176012
version: 1
was generated by: iGEM to SBOL conversion
description: Kozak
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: yeast/ human Kozak plus start codon
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_J176012_sequence/1

STOP
type: ComponentDefinition
persistent identity: BBa J176016
display id: BBa_J176016
version: 1
was generated by: iGEM to SBOL conversion
description: STOP
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: contains stop codons TAA and TGA in frame
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_J176016_sequence/1

PEST
type: ComponentDefinition
persistent identity: BBa J176018
display id: BBa_J176018
version: 1
was generated by: iGEM to SBOL conversion
description: PEST
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: degradation tag
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_J176018_sequence/1

Gal4DB
type: ComponentDefinition
persistent identity: BBa J176020
display id: BBa_J176020
version: 1
was generated by: iGEM to SBOL conversion
description: Gal4 DB
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Gal4 DNA binding domain (remade by KAH)
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J176020_sequence/1

HPK
type: ComponentDefinition
persistent identity: BBa J176028
display id: BBa_J176028
version: 1
was generated by: iGEM to SBOL conversion
description: HPK
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: mutated SpeI
sequence annotation: mutated PstI
sequence: https://synbiohub.org/public/igem/BBa_J176028_sequence/1

mutated SpeI
type: SequenceAnnotation
persistent identity: annotation2166553
display id: annotation2166553
version: 1
top level: HPK
location: https://synbiohub.org/public/igem/BBa_J176028/annotation2166553/range2166553/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J176028/annotation2166553/range2166553/1
type: Range
persistent identity: range2166553
display id: range2166553
version: 1
top level: HPK
direction: direction/forward
start: 253
end: 253
orientation: inline

mutated PstI
type: SequenceAnnotation
persistent identity: annotation2166554
display id: annotation2166554
version: 1
top level: HPK
location: https://synbiohub.org/public/igem/BBa_J176028/annotation2166554/range2166554/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J176028/annotation2166554/range2166554/1
type: Range
persistent identity: range2166554
display id: range2166554
version: 1
top level: HPK
direction: direction/forward
start: 511
end: 511
orientation: inline

Spacer
type: ComponentDefinition
persistent identity: BBa J176039
display id: BBa_J176039
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Noncoding spacer to put between TF binding site and TATA
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin, Karmella Haynes
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J176039_sequence/1

BBa_S04730
type: ComponentDefinition
persistent identity: BBa S04730
display id: BBa_S04730
version: 1
was generated by: iGEM to SBOL conversion
description: J176025:J176009
created: 2011-10-14T11:00:00Z
modified: 2015-05-08T01:14:44Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J176025
component: BBa_J176009
component: BBa_J176041
sequence annotation: BBa_J176025
sequence annotation: BBa_J176041
sequence annotation: BBa_J176009
sequence: https://synbiohub.org/public/igem/BBa_S04730_sequence/1

BBa_J176025
type: Component
persistent identity: component2158521
display id: component2158521
version: 1
top level: BBa_S04730
definition: miRts luc
access: public

BBa_J176009
type: Component
persistent identity: component2158523
display id: component2158523
version: 1
top level: BBa_S04730
definition: polyA
access: public

BBa_J176041
type: Component
persistent identity: component2158522
display id: component2158522
version: 1
top level: BBa_S04730
definition: scar
access: public

BBa_J176025
type: SequenceAnnotation
persistent identity: annotation2158521
display id: annotation2158521
version: 1
top level: BBa_S04730
location: https://synbiohub.org/public/igem/BBa_S04730/annotation2158521/range2158521/1
component: BBa_J176025

https://synbiohub.org/public/igem/BBa_S04730/annotation2158521/range2158521/1
type: Range
persistent identity: range2158521
display id: range2158521
version: 1
top level: BBa_S04730
direction: direction/reverse
start: 1
end: 187
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158522
display id: annotation2158522
version: 1
top level: BBa_S04730
location: https://synbiohub.org/public/igem/BBa_S04730/annotation2158522/range2158522/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04730/annotation2158522/range2158522/1
type: Range
persistent identity: range2158522
display id: range2158522
version: 1
top level: BBa_S04730
direction: direction/reverse
start: 188
end: 193
orientation: inline

BBa_J176009
type: SequenceAnnotation
persistent identity: annotation2158523
display id: annotation2158523
version: 1
top level: BBa_S04730
location: https://synbiohub.org/public/igem/BBa_S04730/annotation2158523/range2158523/1
component: BBa_J176009

https://synbiohub.org/public/igem/BBa_S04730/annotation2158523/range2158523/1
type: Range
persistent identity: range2158523
display id: range2158523
version: 1
top level: BBa_S04730
direction: direction/reverse
start: 194
end: 418
orientation: inline

miRts CCND
type: ComponentDefinition
persistent identity: BBa J176134
display id: BBa_J176134
version: 1
was generated by: iGEM to SBOL conversion
description: miRts CCND1
created: 2011-12-10T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: De novo DNA synthesis (IDT DNA)
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Contains three short repeats
star: false
creator: Karmella Haynes, Francesca Ceroni
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: CCND1 miR-34 target
sequence: https://synbiohub.org/public/igem/BBa_J176134_sequence/1

CCND1 miR-34 target
type: SequenceAnnotation
persistent identity: annotation2166750
display id: annotation2166750
version: 1
top level: miRts CCND
location: https://synbiohub.org/public/igem/BBa_J176134/annotation2166750/range2166750/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J176134/annotation2166750/range2166750/1
type: Range
persistent identity: range2166750
display id: range2166750
version: 1
top level: miRts CCND
direction: direction/forward
start: 1
end: 20
orientation: inline

CCND1 miR-34 target
type: SequenceAnnotation
persistent identity: annotation2166751
display id: annotation2166751
version: 1
top level: miRts CCND
location: https://synbiohub.org/public/igem/BBa_J176134/annotation2166751/range2166751/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J176134/annotation2166751/range2166751/1
type: Range
persistent identity: range2166751
display id: range2166751
version: 1
top level: miRts CCND
direction: direction/forward
start: 21
end: 40
orientation: inline

CCND1 miR-34 target
type: SequenceAnnotation
persistent identity: annotation2166752
display id: annotation2166752
version: 1
top level: miRts CCND
location: https://synbiohub.org/public/igem/BBa_J176134/annotation2166752/range2166752/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176134/annotation2166752/range2166752/1
type: Range
persistent identity: range2166752
display id: range2166752
version: 1
top level: miRts CCND
direction: direction/forward
start: 41
end: 60
orientation: inline

polyA
type: ComponentDefinition
persistent identity: BBa J176009
display id: BBa_J176009
version: 1
was generated by: iGEM to SBOL conversion
description: polyA2
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: BGH polyA signal cloned from pcDNA5/FRT vector
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176009_sequence/1

miRts luc
type: ComponentDefinition
persistent identity: BBa J176025
display id: BBa_J176025
version: 1
was generated by: iGEM to SBOL conversion
description: miRts luc
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: miRNA target for luciferase RNAi
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: repeat 1
sequence annotation: repeat 2
sequence annotation: repeat 3
sequence annotation: repeat 4
sequence annotation: repeat 5
sequence: https://synbiohub.org/public/igem/BBa_J176025_sequence/1

repeat 1
type: SequenceAnnotation
persistent identity: annotation2166807
display id: annotation2166807
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166807/range2166807/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176025/annotation2166807/range2166807/1
type: Range
persistent identity: range2166807
display id: range2166807
version: 1
top level: miRts luc
direction: direction/forward
start: 28
end: 46
orientation: inline

repeat 2
type: SequenceAnnotation
persistent identity: annotation2166808
display id: annotation2166808
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166808/range2166808/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176025/annotation2166808/range2166808/1
type: Range
persistent identity: range2166808
display id: range2166808
version: 1
top level: miRts luc
direction: direction/forward
start: 55
end: 73
orientation: inline

repeat 3
type: SequenceAnnotation
persistent identity: annotation2166809
display id: annotation2166809
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166809/range2166809/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J176025/annotation2166809/range2166809/1
type: Range
persistent identity: range2166809
display id: range2166809
version: 1
top level: miRts luc
direction: direction/forward
start: 82
end: 100
orientation: inline

repeat 4
type: SequenceAnnotation
persistent identity: annotation2166810
display id: annotation2166810
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166810/range2166810/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J176025/annotation2166810/range2166810/1
type: Range
persistent identity: range2166810
display id: range2166810
version: 1
top level: miRts luc
direction: direction/forward
start: 109
end: 127
orientation: inline

repeat 5
type: SequenceAnnotation
persistent identity: annotation2166811
display id: annotation2166811
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166811/range2166811/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176025/annotation2166811/range2166811/1
type: Range
persistent identity: range2166811
display id: range2166811
version: 1
top level: miRts luc
direction: direction/forward
start: 135
end: 153
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa J176041
display id: BBa_J176041
version: 1
was generated by: iGEM to SBOL conversion
description: Silver scar
created: 2011-10-04T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard).
owned by: james
owned by: myers
mutable description: Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard).
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts.
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J176041_sequence/1

https://synbiohub.org/public/igem/BBa_J176137_sequence/1
type: Sequence
persistent identity: BBa J176137 sequence
display id: BBa_J176137_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccgtgacgccggtgacgccgactagagtgacgccggtgacgccgcccgccgccaccatggaggtgacgccggtgacgccgactagagtgacgccggtgacgccgatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcgtgacgccggtgacgccgactagagtgacgccggtgacgccggcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaatagtgacgccggtgacgccgactagagtgacgccggtgacgccggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176005_sequence/1
type: Sequence
persistent identity: BBa J176005 sequence
display id: BBa_J176005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176008_sequence/1
type: Sequence
persistent identity: BBa J176008 sequence
display id: BBa_J176008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccaagaaaaagcgcaaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176012_sequence/1
type: Sequence
persistent identity: BBa J176012 sequence
display id: BBa_J176012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176016_sequence/1
type: Sequence
persistent identity: BBa J176016 sequence
display id: BBa_J176016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaaagctgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176018_sequence/1
type: Sequence
persistent identity: BBa J176018 sequence
display id: BBa_J176018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176020_sequence/1
type: Sequence
persistent identity: BBa J176020 sequence
display id: BBa_J176020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176028_sequence/1
type: Sequence
persistent identity: BBa J176028 sequence
display id: BBa_J176028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176039_sequence/1
type: Sequence
persistent identity: BBa J176039 sequence
display id: BBa_J176039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgacgccggtgacgccgactagagtgacgccggtgacgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04730_sequence/1
type: Sequence
persistent identity: BBa S04730 sequence
display id: BBa_S04730_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176134_sequence/1
type: Sequence
persistent identity: BBa J176134 sequence
display id: BBa_J176134_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acaatgtcatatactgccatacaatgtcatatactgccatacaatgtcatatactgccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176009_sequence/1
type: Sequence
persistent identity: BBa J176009 sequence
display id: BBa_J176009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176025_sequence/1
type: Sequence
persistent identity: BBa J176025 sequence
display id: BBa_J176025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176041_sequence/1
type: Sequence
persistent identity: BBa J176041 sequence
display id: BBa_J176041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z