LOCUS BBa_J176138 3969 bp DNA linear 22-Nov-2024 DEFINITION HPK-RFP-miRts CDK6 ACCESSION BBa_J176138 VERSION BBa_J176138.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 525..566 /label=BBa_J176039 RBS 575..592 /label=BBa_J176012 misc_feature 601..642 /label=BBa_J176039 misc_feature 649..1107 /label=BBa_J176020 misc_feature 1116..1157 /label=BBa_J176039 misc_feature 1166..1699 /label=BBa_J176018 misc_feature 1708..1749 /label=BBa_J176039 misc_feature 1758..2462 /label=BBa_J176005 misc_feature 2471..2512 /label=BBa_J176039 misc_feature 2521..3225 /label=BBa_J176005 misc_feature 3234..3275 /label=BBa_J176039 misc_feature 3284..3304 /label=BBa_J176008 misc_feature 3313..3354 /label=BBa_J176039 terminator 3363..3375 /label=BBa_J176016 misc_feature 3384..3425 /label=BBa_J176039 misc_feature 3434..3453 /label=CDK6 miR-34 target misc_feature 3454..3473 /label=CDK6 miR-34 target misc_feature 3474..3493 /label=CDK6 miR-34 target misc_feature 3434..3493 /label=BBa_J176135 misc_feature 3502..3543 /label=BBa_J176039 misc_feature 3579..3597 /label=repeat 1 misc_feature 3606..3624 /label=repeat 2 misc_feature 3633..3651 /label=repeat 3 misc_feature 3660..3678 /label=repeat 4 misc_feature 3686..3704 /label=repeat 5 misc_feature 3552..3738 /label=BBa_J176025 misc_feature 3739..3744 /label=BBa_J176041 misc_feature 3745..3969 /label=BBa_J176009 misc_feature 3552..3969 /label=BBa_S04730 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagccctact agaggtgacg ccggtgacgc 541 cgactagagt gacgccggtg acgccgtact agagcccgcc gccaccatgg agtactagag 601 gtgacgccgg tgacgccgac tagagtgacg ccggtgacgc cgtactagat gaagctactg 661 tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa 721 aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc 781 aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg 841 gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat 901 tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa 961 gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag 1021 catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg 1081 actgtatcgc cggaatttcc ggggatctac tagaggtgac gccggtgacg ccgactagag 1141 tgacgccggt gacgccgtac tagaggcatc caacttgaac atttcgagaa agcttaccat 1201 atcaacccca tcatgctctt tcgaaaattc aaatagcaca tccattcctt cgcccgcttc 1261 ctcatctcaa agccacactc caatgagaaa catgagctca ctctctgata acagcgtttt 1321 cagccggaat atggaacaat catcaccaat cactccaagt atgtaccaat ttggtcagca 1381 gcagtcaaac agtatatgtg gtagcaccgt tagtgtgaat agtctggtga atacaaataa 1441 caaacaaagg atctacgaac aaatcacggg tcctaacagc aataacgcaa ccaatgatta 1501 tattgatttg ctaaacctaa atgagtctaa caaggaaaac caaaatcccg caacggcgca 1561 ttacctcaat gggggcccac ccaagacaag cttcattaac catggaatgt tcccctcgcc 1621 aactgggacc ataaatagcg gtaaatctag cagtgcctca tctttaattt cttttggtat 1681 gggcaatacc caagtaatat actagaggtg acgccggtga cgccgactag agtgacgccg 1741 gtgacgccgt actagaggtg agcaagggcg aggaggataa catggccatc atcaaggagt 1801 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg 1861 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg 1921 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg 1981 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct 2041 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact 2101 cctccttgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct 2161 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt 2221 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg 2281 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctacccg 2341 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg 2401 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca 2461 agtactagag gtgacgccgg tgacgccgac tagagtgacg ccggtgacgc cgtactagag 2521 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg 2581 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 2641 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc 2701 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc 2761 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 2821 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc 2881 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg 2941 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc 3001 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag 3061 gtcaagacca cctacaaggc caagaagccc gtgcagctac ccggcgccta caacgtcaac 3121 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc 3181 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtacta gaggtgacgc 3241 cggtgacgcc gactagagtg acgccggtga cgccgtacta gagcccaaga aaaagcgcaa 3301 ggtatactag aggtgacgcc ggtgacgccg actagagtga cgccggtgac gccgtactag 3361 aggcgtaaag ctgagtacta gaggtgacgc cggtgacgcc gactagagtg acgccggtga 3421 cgccgtacta gagcagtgtg gaaattcact gcccagtgtg gaaattcact gcccagtgtg 3481 gaaattcact gcctactaga ggtgacgccg gtgacgccga ctagagtgac gccggtgacg 3541 ccgtactaga gatagttgtt taaacgagct cgctagcaaa tcaagagtcc tcataaagga 3601 attaaaatca agagtcctca taaagtctag caaatcaaga gtcctcataa agctagacaa 3661 atcaagagtc ctcataaata attccaatca agagtcctca taaagtcgac ctgcaagcat 3721 gcaagctgat ccggctgcac tagactgtgc cttctagttg ccagccatct gttgtttgcc 3781 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 3841 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 3901 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 3961 gctctatgg //