BBa_J176009 1 polyA polyA2 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA BGH polyA signal cloned from pcDNA5/FRT vector false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176016 1 STOP STOP 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA contains stop codons TAA and TGA in frame false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176012 1 K Kozak 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 yeast/ human Kozak plus start codon false false _863_ 0 10865 9 In stock false TBA false Brady Laughlin BBa_J176041 1 scar Silver scar 2011-10-04T11:00:00Z 2015-08-31T04:08:34Z This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard). Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard). false false _863_ 0 1144 61 Not in stock false The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts. false Karmella Haynes BBa_J176025 1 miRts luc miRts luc 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA miRNA target for luciferase RNAi false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166808 1 repeat 2 range2166808 1 55 73 annotation2166807 1 repeat 1 range2166807 1 28 46 annotation2166811 1 repeat 5 range2166811 1 135 153 annotation2166810 1 repeat 4 range2166810 1 109 127 annotation2166809 1 repeat 3 range2166809 1 82 100 BBa_S04730 1 BBa_S04730 J176025:J176009 2011-10-14T11:00:00Z 2015-05-08T01:14:44Z false false _9_ 0 10865 9 Not in stock false false Brady Laughlin component2158523 1 BBa_J176009 component2158522 1 BBa_J176041 component2158521 1 BBa_J176025 annotation2158523 1 BBa_J176009 range2158523 1 194 418 annotation2158521 1 BBa_J176025 range2158521 1 1 187 annotation2158522 1 BBa_J176041 range2158522 1 188 193 BBa_J176020 1 Gal4DB Gal4 DB 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Gal4 DNA binding domain (remade by KAH) false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes BBa_J176138 1 BBa_J176138 HPK-RFP-miRts CDK6 2011-12-10T12:00:00Z 2015-08-31T04:08:35Z Constructed by Daniel Flicker TBA false false _863_ 0 1144 61 Not in stock false TBA false Karmella Haynes, Daniel Flicker component2238525 1 BBa_J176135 component2238520 1 BBa_J176016 component2238518 1 BBa_J176008 component2238513 1 BBa_J176039 component2238509 1 BBa_J176039 component2238511 1 BBa_J176039 component2238514 1 BBa_J176005 component2238517 1 BBa_J176039 component2238526 1 BBa_J176039 component2238508 1 BBa_J176012 component2238506 1 BBa_J176028 component2238512 1 BBa_J176018 component2238515 1 BBa_J176039 component2238521 1 BBa_J176039 component2238516 1 BBa_J176005 component2238519 1 BBa_J176039 component2238510 1 BBa_J176020 component2238507 1 BBa_J176039 component2238530 1 BBa_S04730 annotation2238530 1 BBa_S04730 range2238530 1 3552 3969 annotation2238521 1 BBa_J176039 range2238521 1 3384 3425 annotation2238512 1 BBa_J176018 range2238512 1 1166 1699 annotation2238518 1 BBa_J176008 range2238518 1 3284 3304 annotation2238508 1 BBa_J176012 range2238508 1 575 592 annotation2238515 1 BBa_J176039 range2238515 1 2471 2512 annotation2238506 1 BBa_J176028 range2238506 1 1 516 annotation2238507 1 BBa_J176039 range2238507 1 525 566 annotation2238525 1 BBa_J176135 range2238525 1 3434 3493 annotation2238514 1 BBa_J176005 range2238514 1 1758 2462 annotation2238510 1 BBa_J176020 range2238510 1 649 1107 annotation2238513 1 BBa_J176039 range2238513 1 1708 1749 annotation2238509 1 BBa_J176039 range2238509 1 601 642 annotation2238511 1 BBa_J176039 range2238511 1 1116 1157 annotation2238526 1 BBa_J176039 range2238526 1 3502 3543 annotation2238517 1 BBa_J176039 range2238517 1 3234 3275 annotation2238519 1 BBa_J176039 range2238519 1 3313 3354 annotation2238520 1 BBa_J176016 range2238520 1 3363 3375 annotation2238516 1 BBa_J176005 range2238516 1 2521 3225 BBa_J176039 1 Spacer Spacer 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Noncoding spacer to put between TF binding site and TATA false false _863_ 0 10865 9 Not in stock false TBA false Brady Laughlin, Karmella Haynes BBa_J176005 1 mCh mCh 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Humanized mCherry fluorescent protein false false _863_ 0 10865 9 It's complicated In stock false TBA false Karmella Haynes BBa_J176018 1 PEST PEST 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA degradation tag false false _863_ 0 10865 9 It's complicated false TBA false Karmella Haynes BBa_J176008 1 NLS NLS 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA Released HQ 2013 SV40 nuclear localization sequence false false _863_ 0 10865 9 In stock false TBA false Karmella Haynes BBa_J176028 1 HPK HPK 2011-09-28T11:00:00Z 2015-08-31T04:08:33Z TBA constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO false false _863_ 0 10865 9 Not in stock false TBA false Karmella Haynes annotation2166554 1 mutated PstI range2166554 1 511 511 annotation2166553 1 mutated SpeI range2166553 1 253 253 BBa_J176135 1 miRts CDK6 miRts CDK6 2011-12-10T12:00:00Z 2015-08-31T04:08:35Z De novo synthesis (IDT DNA) TBA false false _863_ 0 1144 61 Not in stock false Contains three short repeats false Karmella Haynes, Francesca Ceroni annotation2166754 1 CDK6 miR-34 target range2166754 1 21 40 annotation2166753 1 CDK6 miR-34 target range2166753 1 1 20 annotation2166755 1 CDK6 miR-34 target range2166755 1 41 60 BBa_J176138_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccctactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagagcccgccgccaccatggagtactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatctactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagaggcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaatatactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagaggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagaggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagagcccaagaaaaagcgcaaggtatactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagaggcgtaaagctgagtactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagagcagtgtggaaattcactgcccagtgtggaaattcactgcccagtgtggaaattcactgcctactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagagatagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176018_sequence 1 gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata BBa_J176005_sequence 1 gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag BBa_J176041_sequence 1 actaga BBa_J176135_sequence 1 cagtgtggaaattcactgcccagtgtggaaattcactgcccagtgtggaaattcactgcc BBa_J176009_sequence 1 ctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176020_sequence 1 atgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatc BBa_J176016_sequence 1 gcgtaaagctgag BBa_J176012_sequence 1 cccgccgccaccatggag BBa_J176039_sequence 1 gtgacgccggtgacgccgactagagtgacgccggtgacgccg BBa_J176025_sequence 1 atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgc BBa_S04730_sequence 1 atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg BBa_J176028_sequence 1 ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc BBa_J176008_sequence 1 cccaagaaaaagcgcaaggta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z