BBa_J176138
type: ComponentDefinition
persistent identity: BBa J176138
display id: BBa_J176138
version: 1
was generated by: iGEM to SBOL conversion
description: HPK-RFP-miRts CDK6
created: 2011-12-10T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: Constructed by Daniel Flicker
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes, Daniel Flicker
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J176039
component: BBa_J176005
component: BBa_J176020
component: BBa_J176018
component: BBa_J176135
component: BBa_S04730
component: BBa_J176008
component: BBa_J176016
component: BBa_J176028
component: BBa_J176012
sequence annotation: BBa_J176028
sequence annotation: BBa_J176039
sequence annotation: BBa_J176012
sequence annotation: BBa_J176020
sequence annotation: BBa_J176018
sequence annotation: BBa_J176005
sequence annotation: BBa_J176008
sequence annotation: BBa_J176016
sequence annotation: BBa_J176135
sequence annotation: BBa_S04730
sequence: https://synbiohub.org/public/igem/BBa_J176138_sequence/1

BBa_J176039
type: Component
persistent identity: component2238515
display id: component2238515
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176005
type: Component
persistent identity: component2238514
display id: component2238514
version: 1
top level: BBa_J176138
definition: mCh
access: public

BBa_J176039
type: Component
persistent identity: component2238517
display id: component2238517
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176005
type: Component
persistent identity: component2238516
display id: component2238516
version: 1
top level: BBa_J176138
definition: mCh
access: public

BBa_J176039
type: Component
persistent identity: component2238511
display id: component2238511
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176020
type: Component
persistent identity: component2238510
display id: component2238510
version: 1
top level: BBa_J176138
definition: Gal4DB
access: public

BBa_J176039
type: Component
persistent identity: component2238513
display id: component2238513
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176018
type: Component
persistent identity: component2238512
display id: component2238512
version: 1
top level: BBa_J176138
definition: PEST
access: public

BBa_J176039
type: Component
persistent identity: component2238526
display id: component2238526
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176135
type: Component
persistent identity: component2238525
display id: component2238525
version: 1
top level: BBa_J176138
definition: miRts CDK6
access: public

BBa_S04730
type: Component
persistent identity: component2238530
display id: component2238530
version: 1
top level: BBa_J176138
definition: BBa_S04730
access: public

BBa_J176039
type: Component
persistent identity: component2238519
display id: component2238519
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176008
type: Component
persistent identity: component2238518
display id: component2238518
version: 1
top level: BBa_J176138
definition: NLS
access: public

BBa_J176039
type: Component
persistent identity: component2238521
display id: component2238521
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176016
type: Component
persistent identity: component2238520
display id: component2238520
version: 1
top level: BBa_J176138
definition: STOP
access: public

BBa_J176039
type: Component
persistent identity: component2238507
display id: component2238507
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176028
type: Component
persistent identity: component2238506
display id: component2238506
version: 1
top level: BBa_J176138
definition: HPK
access: public

BBa_J176039
type: Component
persistent identity: component2238509
display id: component2238509
version: 1
top level: BBa_J176138
definition: Spacer
access: public

BBa_J176012
type: Component
persistent identity: component2238508
display id: component2238508
version: 1
top level: BBa_J176138
definition: K
access: public

BBa_J176028
type: SequenceAnnotation
persistent identity: annotation2238506
display id: annotation2238506
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238506/range2238506/1
component: BBa_J176028

https://synbiohub.org/public/igem/BBa_J176138/annotation2238506/range2238506/1
type: Range
persistent identity: range2238506
display id: range2238506
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 1
end: 516
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238507
display id: annotation2238507
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238507/range2238507/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238507/range2238507/1
type: Range
persistent identity: range2238507
display id: range2238507
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 525
end: 566
orientation: inline

BBa_J176012
type: SequenceAnnotation
persistent identity: annotation2238508
display id: annotation2238508
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238508/range2238508/1
component: BBa_J176012

https://synbiohub.org/public/igem/BBa_J176138/annotation2238508/range2238508/1
type: Range
persistent identity: range2238508
display id: range2238508
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 575
end: 592
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238509
display id: annotation2238509
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238509/range2238509/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238509/range2238509/1
type: Range
persistent identity: range2238509
display id: range2238509
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 601
end: 642
orientation: inline

BBa_J176020
type: SequenceAnnotation
persistent identity: annotation2238510
display id: annotation2238510
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238510/range2238510/1
component: BBa_J176020

https://synbiohub.org/public/igem/BBa_J176138/annotation2238510/range2238510/1
type: Range
persistent identity: range2238510
display id: range2238510
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 649
end: 1107
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238511
display id: annotation2238511
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238511/range2238511/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238511/range2238511/1
type: Range
persistent identity: range2238511
display id: range2238511
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 1116
end: 1157
orientation: inline

BBa_J176018
type: SequenceAnnotation
persistent identity: annotation2238512
display id: annotation2238512
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238512/range2238512/1
component: BBa_J176018

https://synbiohub.org/public/igem/BBa_J176138/annotation2238512/range2238512/1
type: Range
persistent identity: range2238512
display id: range2238512
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 1166
end: 1699
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238513
display id: annotation2238513
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238513/range2238513/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238513/range2238513/1
type: Range
persistent identity: range2238513
display id: range2238513
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 1708
end: 1749
orientation: inline

BBa_J176005
type: SequenceAnnotation
persistent identity: annotation2238514
display id: annotation2238514
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238514/range2238514/1
component: BBa_J176005

https://synbiohub.org/public/igem/BBa_J176138/annotation2238514/range2238514/1
type: Range
persistent identity: range2238514
display id: range2238514
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 1758
end: 2462
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238515
display id: annotation2238515
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238515/range2238515/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238515/range2238515/1
type: Range
persistent identity: range2238515
display id: range2238515
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 2471
end: 2512
orientation: inline

BBa_J176005
type: SequenceAnnotation
persistent identity: annotation2238516
display id: annotation2238516
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238516/range2238516/1
component: BBa_J176005

https://synbiohub.org/public/igem/BBa_J176138/annotation2238516/range2238516/1
type: Range
persistent identity: range2238516
display id: range2238516
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 2521
end: 3225
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238517
display id: annotation2238517
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238517/range2238517/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238517/range2238517/1
type: Range
persistent identity: range2238517
display id: range2238517
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 3234
end: 3275
orientation: inline

BBa_J176008
type: SequenceAnnotation
persistent identity: annotation2238518
display id: annotation2238518
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238518/range2238518/1
component: BBa_J176008

https://synbiohub.org/public/igem/BBa_J176138/annotation2238518/range2238518/1
type: Range
persistent identity: range2238518
display id: range2238518
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 3284
end: 3304
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238519
display id: annotation2238519
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238519/range2238519/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238519/range2238519/1
type: Range
persistent identity: range2238519
display id: range2238519
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 3313
end: 3354
orientation: inline

BBa_J176016
type: SequenceAnnotation
persistent identity: annotation2238520
display id: annotation2238520
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238520/range2238520/1
component: BBa_J176016

https://synbiohub.org/public/igem/BBa_J176138/annotation2238520/range2238520/1
type: Range
persistent identity: range2238520
display id: range2238520
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 3363
end: 3375
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238521
display id: annotation2238521
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238521/range2238521/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238521/range2238521/1
type: Range
persistent identity: range2238521
display id: range2238521
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 3384
end: 3425
orientation: inline

BBa_J176135
type: SequenceAnnotation
persistent identity: annotation2238525
display id: annotation2238525
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238525/range2238525/1
component: BBa_J176135

https://synbiohub.org/public/igem/BBa_J176138/annotation2238525/range2238525/1
type: Range
persistent identity: range2238525
display id: range2238525
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 3434
end: 3493
orientation: inline

BBa_J176039
type: SequenceAnnotation
persistent identity: annotation2238526
display id: annotation2238526
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238526/range2238526/1
component: BBa_J176039

https://synbiohub.org/public/igem/BBa_J176138/annotation2238526/range2238526/1
type: Range
persistent identity: range2238526
display id: range2238526
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 3502
end: 3543
orientation: inline

BBa_S04730
type: SequenceAnnotation
persistent identity: annotation2238530
display id: annotation2238530
version: 1
top level: BBa_J176138
location: https://synbiohub.org/public/igem/BBa_J176138/annotation2238530/range2238530/1
component: BBa_S04730

https://synbiohub.org/public/igem/BBa_J176138/annotation2238530/range2238530/1
type: Range
persistent identity: range2238530
display id: range2238530
version: 1
top level: BBa_J176138
direction: direction/reverse
start: 3552
end: 3969
orientation: inline

mCh
type: ComponentDefinition
persistent identity: BBa J176005
display id: BBa_J176005
version: 1
was generated by: iGEM to SBOL conversion
description: mCh
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Humanized mCherry fluorescent protein
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
sample status: In stock
status: status/Available
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_J176005_sequence/1

NLS
type: ComponentDefinition
persistent identity: BBa J176008
display id: BBa_J176008
version: 1
was generated by: iGEM to SBOL conversion
description: NLS
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: SV40 nuclear localization sequence
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176008_sequence/1

STOP
type: ComponentDefinition
persistent identity: BBa J176016
display id: BBa_J176016
version: 1
was generated by: iGEM to SBOL conversion
description: STOP
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: contains stop codons TAA and TGA in frame
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_J176016_sequence/1

PEST
type: ComponentDefinition
persistent identity: BBa J176018
display id: BBa_J176018
version: 1
was generated by: iGEM to SBOL conversion
description: PEST
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: degradation tag
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence: https://synbiohub.org/public/igem/BBa_J176018_sequence/1

Gal4DB
type: ComponentDefinition
persistent identity: BBa J176020
display id: BBa_J176020
version: 1
was generated by: iGEM to SBOL conversion
description: Gal4 DB
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Gal4 DNA binding domain (remade by KAH)
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_J176020_sequence/1

HPK
type: ComponentDefinition
persistent identity: BBa J176028
display id: BBa_J176028
version: 1
was generated by: iGEM to SBOL conversion
description: HPK
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: constitute human phosphoglycerate kinase promoter; PCR cloned from pmirGLO
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: mutated SpeI
sequence annotation: mutated PstI
sequence: https://synbiohub.org/public/igem/BBa_J176028_sequence/1

mutated SpeI
type: SequenceAnnotation
persistent identity: annotation2166553
display id: annotation2166553
version: 1
top level: HPK
location: https://synbiohub.org/public/igem/BBa_J176028/annotation2166553/range2166553/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J176028/annotation2166553/range2166553/1
type: Range
persistent identity: range2166553
display id: range2166553
version: 1
top level: HPK
direction: direction/forward
start: 253
end: 253
orientation: inline

mutated PstI
type: SequenceAnnotation
persistent identity: annotation2166554
display id: annotation2166554
version: 1
top level: HPK
location: https://synbiohub.org/public/igem/BBa_J176028/annotation2166554/range2166554/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J176028/annotation2166554/range2166554/1
type: Range
persistent identity: range2166554
display id: range2166554
version: 1
top level: HPK
direction: direction/forward
start: 511
end: 511
orientation: inline

Spacer
type: ComponentDefinition
persistent identity: BBa J176039
display id: BBa_J176039
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: Noncoding spacer to put between TF binding site and TATA
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin, Karmella Haynes
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J176039_sequence/1

BBa_S04730
type: ComponentDefinition
persistent identity: BBa S04730
display id: BBa_S04730
version: 1
was generated by: iGEM to SBOL conversion
description: J176025:J176009
created: 2011-10-14T11:00:00Z
modified: 2015-05-08T01:14:44Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Brady Laughlin
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_J176025
component: BBa_J176009
component: BBa_J176041
sequence annotation: BBa_J176025
sequence annotation: BBa_J176041
sequence annotation: BBa_J176009
sequence: https://synbiohub.org/public/igem/BBa_S04730_sequence/1

BBa_J176025
type: Component
persistent identity: component2158521
display id: component2158521
version: 1
top level: BBa_S04730
definition: miRts luc
access: public

BBa_J176009
type: Component
persistent identity: component2158523
display id: component2158523
version: 1
top level: BBa_S04730
definition: polyA
access: public

BBa_J176041
type: Component
persistent identity: component2158522
display id: component2158522
version: 1
top level: BBa_S04730
definition: scar
access: public

BBa_J176025
type: SequenceAnnotation
persistent identity: annotation2158521
display id: annotation2158521
version: 1
top level: BBa_S04730
location: https://synbiohub.org/public/igem/BBa_S04730/annotation2158521/range2158521/1
component: BBa_J176025

https://synbiohub.org/public/igem/BBa_S04730/annotation2158521/range2158521/1
type: Range
persistent identity: range2158521
display id: range2158521
version: 1
top level: BBa_S04730
direction: direction/reverse
start: 1
end: 187
orientation: inline

BBa_J176041
type: SequenceAnnotation
persistent identity: annotation2158522
display id: annotation2158522
version: 1
top level: BBa_S04730
location: https://synbiohub.org/public/igem/BBa_S04730/annotation2158522/range2158522/1
component: BBa_J176041

https://synbiohub.org/public/igem/BBa_S04730/annotation2158522/range2158522/1
type: Range
persistent identity: range2158522
display id: range2158522
version: 1
top level: BBa_S04730
direction: direction/reverse
start: 188
end: 193
orientation: inline

BBa_J176009
type: SequenceAnnotation
persistent identity: annotation2158523
display id: annotation2158523
version: 1
top level: BBa_S04730
location: https://synbiohub.org/public/igem/BBa_S04730/annotation2158523/range2158523/1
component: BBa_J176009

https://synbiohub.org/public/igem/BBa_S04730/annotation2158523/range2158523/1
type: Range
persistent identity: range2158523
display id: range2158523
version: 1
top level: BBa_S04730
direction: direction/reverse
start: 194
end: 418
orientation: inline

miRts CDK6
type: ComponentDefinition
persistent identity: BBa J176135
display id: BBa_J176135
version: 1
was generated by: iGEM to SBOL conversion
description: miRts CDK6
created: 2011-12-10T12:00:00Z
modified: 2015-08-31T04:08:35Z
mutable provenance: De novo synthesis (IDT DNA)
owned by: james
owned by: myers
mutable description: TBA
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Contains three short repeats
star: false
creator: Karmella Haynes, Francesca Ceroni
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: CDK6 miR-34 target
sequence: https://synbiohub.org/public/igem/BBa_J176135_sequence/1

CDK6 miR-34 target
type: SequenceAnnotation
persistent identity: annotation2166753
display id: annotation2166753
version: 1
top level: miRts CDK6
location: https://synbiohub.org/public/igem/BBa_J176135/annotation2166753/range2166753/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J176135/annotation2166753/range2166753/1
type: Range
persistent identity: range2166753
display id: range2166753
version: 1
top level: miRts CDK6
direction: direction/forward
start: 1
end: 20
orientation: inline

CDK6 miR-34 target
type: SequenceAnnotation
persistent identity: annotation2166754
display id: annotation2166754
version: 1
top level: miRts CDK6
location: https://synbiohub.org/public/igem/BBa_J176135/annotation2166754/range2166754/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J176135/annotation2166754/range2166754/1
type: Range
persistent identity: range2166754
display id: range2166754
version: 1
top level: miRts CDK6
direction: direction/forward
start: 21
end: 40
orientation: inline

CDK6 miR-34 target
type: SequenceAnnotation
persistent identity: annotation2166755
display id: annotation2166755
version: 1
top level: miRts CDK6
location: https://synbiohub.org/public/igem/BBa_J176135/annotation2166755/range2166755/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_J176135/annotation2166755/range2166755/1
type: Range
persistent identity: range2166755
display id: range2166755
version: 1
top level: miRts CDK6
direction: direction/forward
start: 41
end: 60
orientation: inline

K
type: ComponentDefinition
persistent identity: BBa J176012
display id: BBa_J176012
version: 1
was generated by: iGEM to SBOL conversion
description: Kozak
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: yeast/ human Kozak plus start codon
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TBA
star: false
creator: Brady Laughlin
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J176012_sequence/1

polyA
type: ComponentDefinition
persistent identity: BBa J176009
display id: BBa_J176009
version: 1
was generated by: iGEM to SBOL conversion
description: polyA2
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: BGH polyA signal cloned from pcDNA5/FRT vector
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J176009_sequence/1

miRts luc
type: ComponentDefinition
persistent identity: BBa J176025
display id: BBa_J176025
version: 1
was generated by: iGEM to SBOL conversion
description: miRts luc
created: 2011-09-28T11:00:00Z
modified: 2015-08-31T04:08:33Z
mutable provenance: TBA
owned by: james
owned by: myers
mutable description: miRNA target for luciferase RNAi
discontinued: false
dominant: false
experience: experience/Works
group u list: _863_
m user id: 0
owner id: 10865
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TBA
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: repeat 1
sequence annotation: repeat 2
sequence annotation: repeat 3
sequence annotation: repeat 4
sequence annotation: repeat 5
sequence: https://synbiohub.org/public/igem/BBa_J176025_sequence/1

repeat 1
type: SequenceAnnotation
persistent identity: annotation2166807
display id: annotation2166807
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166807/range2166807/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176025/annotation2166807/range2166807/1
type: Range
persistent identity: range2166807
display id: range2166807
version: 1
top level: miRts luc
direction: direction/forward
start: 28
end: 46
orientation: inline

repeat 2
type: SequenceAnnotation
persistent identity: annotation2166808
display id: annotation2166808
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166808/range2166808/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176025/annotation2166808/range2166808/1
type: Range
persistent identity: range2166808
display id: range2166808
version: 1
top level: miRts luc
direction: direction/forward
start: 55
end: 73
orientation: inline

repeat 3
type: SequenceAnnotation
persistent identity: annotation2166809
display id: annotation2166809
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166809/range2166809/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J176025/annotation2166809/range2166809/1
type: Range
persistent identity: range2166809
display id: range2166809
version: 1
top level: miRts luc
direction: direction/forward
start: 82
end: 100
orientation: inline

repeat 4
type: SequenceAnnotation
persistent identity: annotation2166810
display id: annotation2166810
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166810/range2166810/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J176025/annotation2166810/range2166810/1
type: Range
persistent identity: range2166810
display id: range2166810
version: 1
top level: miRts luc
direction: direction/forward
start: 109
end: 127
orientation: inline

repeat 5
type: SequenceAnnotation
persistent identity: annotation2166811
display id: annotation2166811
version: 1
top level: miRts luc
location: https://synbiohub.org/public/igem/BBa_J176025/annotation2166811/range2166811/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J176025/annotation2166811/range2166811/1
type: Range
persistent identity: range2166811
display id: range2166811
version: 1
top level: miRts luc
direction: direction/forward
start: 135
end: 153
orientation: inline

scar
type: ComponentDefinition
persistent identity: BBa J176041
display id: BBa_J176041
version: 1
was generated by: iGEM to SBOL conversion
description: Silver scar
created: 2011-10-04T11:00:00Z
modified: 2015-08-31T04:08:34Z
mutable provenance: This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard).
owned by: james
owned by: myers
mutable description: Six base pair scar between parts that have been assembled using BioBrick assembly standard 23 (Silver standard).
discontinued: false
dominant: false
experience: experience/None
group u list: _863_
m user id: 0
owner id: 1144
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts.
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J176041_sequence/1

https://synbiohub.org/public/igem/BBa_J176138_sequence/1
type: Sequence
persistent identity: BBa J176138 sequence
display id: BBa_J176138_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccctactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagagcccgccgccaccatggagtactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatctactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagaggcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaatatactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagaggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176005_sequence/1
type: Sequence
persistent identity: BBa J176005 sequence
display id: BBa_J176005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176008_sequence/1
type: Sequence
persistent identity: BBa J176008 sequence
display id: BBa_J176008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccaagaaaaagcgcaaggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176016_sequence/1
type: Sequence
persistent identity: BBa J176016 sequence
display id: BBa_J176016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaaagctgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176018_sequence/1
type: Sequence
persistent identity: BBa J176018 sequence
display id: BBa_J176018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176020_sequence/1
type: Sequence
persistent identity: BBa J176020 sequence
display id: BBa_J176020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176028_sequence/1
type: Sequence
persistent identity: BBa J176028 sequence
display id: BBa_J176028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176039_sequence/1
type: Sequence
persistent identity: BBa J176039 sequence
display id: BBa_J176039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgacgccggtgacgccgactagagtgacgccggtgacgccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176012_sequence/1
type: Sequence
persistent identity: BBa J176012 sequence
display id: BBa_J176012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04730_sequence/1
type: Sequence
persistent identity: BBa S04730 sequence
display id: BBa_S04730_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176135_sequence/1
type: Sequence
persistent identity: BBa J176135 sequence
display id: BBa_J176135_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagtgtggaaattcactgcccagtgtggaaattcactgcccagtgtggaaattcactgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176009_sequence/1
type: Sequence
persistent identity: BBa J176009 sequence
display id: BBa_J176009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176025_sequence/1
type: Sequence
persistent identity: BBa J176025 sequence
display id: BBa_J176025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J176041_sequence/1
type: Sequence
persistent identity: BBa J176041 sequence
display id: BBa_J176041_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z