LOCUS BBa_J176139 5946 bp DNA linear 22-Dec-2024 DEFINITION miR-34 BCL2 sensor ACCESSION BBa_J176139 VERSION BBa_J176139.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 616..621 /label=BBa_J176041 misc_feature 622..638 /label=Gal4 misc_feature 641..657 /label=Gal4 misc_feature 660..676 /label=Gal4 misc_feature 679..695 /label=Gal4 misc_feature 698..714 /label=Gal4 misc_feature 622..714 /label=BBa_J176019 misc_feature 1..714 /label=BBa_S04655 misc_feature 715..720 /label=BBa_J176041 RBS 721..738 /label=BBa_J176012 misc_feature 739..744 /label=BBa_J176041 misc_feature 745..1431 /label=BBa_J176022 misc_feature 1432..1437 /label=BBa_J176041 misc_feature 1438..2124 /label=BBa_J176022 misc_feature 2125..2130 /label=BBa_J176041 misc_feature 2131..2151 /label=BBa_J176008 misc_feature 2152..2157 /label=BBa_J176041 terminator 2158..2170 /label=BBa_J176016 misc_feature 745..2170 /label=BBa_S04684 misc_feature 721..2170 /label=BBa_S04738 misc_feature 2171..2176 /label=BBa_J176041 misc_feature 2177..2401 /label=BBa_J176009 misc_feature 721..2401 /label=BBa_S04739 misc_feature 1..2401 /label=BBa_J176091 misc_feature 2402..2443 /label=BBa_J176039 misc_feature 2696..2696 /label=mutated SpeI misc_feature 2954..2954 /label=mutated PstI promoter 2444..2959 /label=BBa_J176028 misc_feature 2960..2965 /label=BBa_J176041 RBS 2966..2983 /label=BBa_J176012 misc_feature 2984..2989 /label=BBa_J176041 misc_feature 2990..3448 /label=BBa_J176020 misc_feature 3449..3454 /label=BBa_J176041 misc_feature 3455..3988 /label=BBa_J176018 misc_feature 3989..3994 /label=BBa_J176041 misc_feature 3995..4699 /label=BBa_J176005 misc_feature 4700..4705 /label=BBa_J176041 misc_feature 4706..5410 /label=BBa_J176005 misc_feature 5411..5416 /label=BBa_J176041 misc_feature 5417..5437 /label=BBa_J176008 misc_feature 5438..5443 /label=BBa_J176041 terminator 5444..5456 /label=BBa_J176016 misc_feature 5457..5462 /label=BBa_J176041 misc_feature 5463..5482 /label=Bcl2 miR-34 target misc_feature 5483..5502 /label=Bcl2 miR-34 target misc_feature 5503..5522 /label=Bcl2 miR-34 target misc_feature 5463..5522 /label=BBa_J176133 misc_feature 5523..5528 /label=BBa_J176041 misc_feature 5556..5574 /label=repeat 1 misc_feature 5583..5601 /label=repeat 2 misc_feature 5610..5628 /label=repeat 3 misc_feature 5637..5655 /label=repeat 4 misc_feature 5663..5681 /label=repeat 5 misc_feature 5529..5715 /label=BBa_J176025 misc_feature 5716..5721 /label=BBa_J176041 misc_feature 5722..5946 /label=BBa_J176009 misc_feature 5529..5946 /label=BBa_S04730 misc_feature 2444..5946 /label=BBa_J176136 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acggagtact gtcctccgag cggagtactg tcctccgagc 661 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgactaga 721 cccgccgcca ccatggagac tagaatggcc ctgtccaaca agttcatcgg cgacgacatg 781 aagatgacct accacatgga cggctgcgtg aacggccact acttcaccgt gaagggcgag 841 ggcagcggca agccctacga gggcacccag acctccacct tcaaggtgac catggccaac 901 ggcggccccc tggccttctc cttcgacatc ctgtccaccg tgttcatgta cggcaaccgc 961 tgcttcaccg cctaccccac cagcatgccc gactacttca agcaggcctt ccccgacggc 1021 atgtcctacg agagaacctt cacctacgag gacggcggcg tggccaccgc cagctgggag 1081 atcagcctga agggcaactg cttcgagcac aagtccacct tccacggcgt gaacttcccc 1141 gccgacggcc ccgtgatggc caagaagacc accggctggg acccctcctt cgagaagatg 1201 accgtgtgcg acggcatctt gaagggcgac gtgaccgcct tcctgatgct gcaaggcggc 1261 ggcaactaca gatgccagtt ccacacctcc tacaagacca agaagcccgt gaccatgccc 1321 cccaaccacg tggtggagca ccgcatcgcc agaaccgacc tggacaaggg cggcaacagc 1381 gtgcagctga ccgagcacgc cgtggcccac atcacctccg tggtgccctt cactagaatg 1441 gccctgtcca acaagttcat cggcgacgac atgaagatga cctaccacat ggacggctgc 1501 gtgaacggcc actacttcac cgtgaagggc gagggcagcg gcaagcccta cgagggcacc 1561 cagacctcca ccttcaaggt gaccatggcc aacggcggcc ccctggcctt ctccttcgac 1621 atcctgtcca ccgtgttcat gtacggcaac cgctgcttca ccgcctaccc caccagcatg 1681 cccgactact tcaagcaggc cttccccgac ggcatgtcct acgagagaac cttcacctac 1741 gaggacggcg gcgtggccac cgccagctgg gagatcagcc tgaagggcaa ctgcttcgag 1801 cacaagtcca ccttccacgg cgtgaacttc cccgccgacg gccccgtgat ggccaagaag 1861 accaccggct gggacccctc cttcgagaag atgaccgtgt gcgacggcat cttgaagggc 1921 gacgtgaccg ccttcctgat gctgcaaggc ggcggcaact acagatgcca gttccacacc 1981 tcctacaaga ccaagaagcc cgtgaccatg ccccccaacc acgtggtgga gcaccgcatc 2041 gccagaaccg acctggacaa gggcggcaac agcgtgcagc tgaccgagca cgccgtggcc 2101 cacatcacct ccgtggtgcc cttcactaga cccaagaaaa agcgcaaggt aactagagcg 2161 taaagctgag actagactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 2221 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 2281 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 2341 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 2401 ggtgacgccg gtgacgccga ctagagtgac gccggtgacg ccgctaccgg gtaggggagg 2461 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct 2521 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc 2581 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc 2641 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcacaagtc 2701 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc 2761 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg 2821 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg 2881 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 2941 gcctttcgac ctggagccca ctagacccgc cgccaccatg gagactagaa tgaagctact 3001 gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga 3061 aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac 3121 caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact 3181 ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga 3241 ttctttacag gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa 3301 agatgccgtc acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca 3361 gcatagaata agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt 3421 gactgtatcg ccggaatttc cggggatcac tagagcatcc aacttgaaca tttcgagaaa 3481 gcttaccata tcaaccccat catgctcttt cgaaaattca aatagcacat ccattccttc 3541 gcccgcttcc tcatctcaaa gccacactcc aatgagaaac atgagctcac tctctgataa 3601 cagcgttttc agccggaata tggaacaatc atcaccaatc actccaagta tgtaccaatt 3661 tggtcagcag cagtcaaaca gtatatgtgg tagcaccgtt agtgtgaata gtctggtgaa 3721 tacaaataac aaacaaagga tctacgaaca aatcacgggt cctaacagca ataacgcaac 3781 caatgattat attgatttgc taaacctaaa tgagtctaac aaggaaaacc aaaatcccgc 3841 aacggcgcat tacctcaatg ggggcccacc caagacaagc ttcattaacc atggaatgtt 3901 cccctcgcca actgggacca taaatagcgg taaatctagc agtgcctcat ctttaatttc 3961 ttttggtatg ggcaataccc aagtaataac tagagtgagc aagggcgagg aggataacat 4021 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 4081 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 4141 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 4201 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 4261 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 4321 gaccgtgacc caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg 4381 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 4441 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 4501 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 4561 gcccgtgcag ctacccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 4621 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 4681 catggacgag ctgtacaaga ctagagtgag caagggcgag gaggataaca tggccatcat 4741 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 4801 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 4861 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 4921 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 4981 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 5041 ccaggactcc tccttgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 5101 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 5161 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 5221 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 5281 gctacccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 5341 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 5401 gctgtacaag actagaccca agaaaaagcg caaggtaact agagcgtaaa gctgagacta 5461 gagaatcagc tatttactgc cagaatcagc tatttactgc cagaatcagc tatttactgc 5521 caactagaat agttgtttaa acgagctcgc tagcaaatca agagtcctca taaaggaatt 5581 aaaatcaaga gtcctcataa agtctagcaa atcaagagtc ctcataaagc tagacaaatc 5641 aagagtcctc ataaataatt ccaatcaaga gtcctcataa agtcgacctg caagcatgca 5701 agctgatccg gctgcactag actgtgcctt ctagttgcca gccatctgtt gtttgcccct 5761 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 5821 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 5881 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 5941 ctatgg //