##gff-version 3
##sequence-region BBa_J176139 1 5946
BBa_J176139	.	sequence_feature	2402	2443	.	+	0	ID=BBa_J176039;Name=BBa_J176039
BBa_J176139	.	engineered_region	2444	5946	.	+	0	ID=BBa_J176136;Name=BBa_J176136
BBa_J176139	.	ribosome_entry_site	2966	2983	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	4700	4705	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	3989	3994	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	polypeptide_domain	2990	3448	.	+	0	ID=BBa_J176020;Name=BBa_J176020;Parent=BBa_J176136
BBa_J176139	.	engineered_region	5529	5946	.	+	0	ID=BBa_S04730;Name=BBa_S04730;Parent=BBa_J176136
BBa_J176139	.	tag	5722	5946	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04730
BBa_J176139	.	sequence_feature	5716	5721	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04730
BBa_J176139	.	mature_transcript_region	5529	5715	.	+	0	ID=BBa_J176025;Name=BBa_J176025;Parent=BBa_S04730
BBa_J176139	.	sequence_feature	5583	5601	.	+	0	ID=annotation2166808;Name=repeat 2;Parent=BBa_J176025
BBa_J176139	.	sequence_feature	5610	5628	.	+	0	ID=annotation2166809;Name=repeat 3;Parent=BBa_J176025
BBa_J176139	.	sequence_feature	5556	5574	.	+	0	ID=annotation2166807;Name=repeat 1;Parent=BBa_J176025
BBa_J176139	.	sequence_feature	5663	5681	.	+	0	ID=annotation2166811;Name=repeat 5;Parent=BBa_J176025
BBa_J176139	.	sequence_feature	5637	5655	.	+	0	ID=annotation2166810;Name=repeat 4;Parent=BBa_J176025
BBa_J176139	.	polypeptide_domain	4706	5410	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	5438	5443	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	5411	5416	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	5523	5528	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	2960	2965	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	5457	5462	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	polypeptide_domain	3995	4699	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_J176136
BBa_J176139	.	promoter	2444	2959	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_J176136
BBa_J176139	.	sequence_alteration	2954	2954	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176139	.	sequence_alteration	2696	2696	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176139	.	tag	5417	5437	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	3449	3454	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	mature_transcript_region	5463	5522	.	+	0	ID=BBa_J176133;Name=BBa_J176133;Parent=BBa_J176136
BBa_J176139	.	non_covalent_binding_site	5463	5482	.	+	0	ID=annotation2166747;Name=Bcl2 miR-34 target;Parent=BBa_J176133
BBa_J176139	.	non_covalent_binding_site	5503	5522	.	+	0	ID=annotation2166749;Name=Bcl2 miR-34 target;Parent=BBa_J176133
BBa_J176139	.	non_covalent_binding_site	5483	5502	.	+	0	ID=annotation2166748;Name=Bcl2 miR-34 target;Parent=BBa_J176133
BBa_J176139	.	terminator	5444	5456	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_J176136
BBa_J176139	.	sequence_feature	2984	2989	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176136
BBa_J176139	.	tag	3455	3988	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_J176136
BBa_J176139	.	engineered_region	1	2401	.	+	0	ID=BBa_J176091;Name=BBa_J176091
BBa_J176139	.	engineered_region	1	714	.	+	0	ID=BBa_S04655;Name=BBa_S04655;Parent=BBa_J176091
BBa_J176139	.	promoter	1	516	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04655
BBa_J176139	.	sequence_alteration	511	511	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176139	.	sequence_alteration	253	253	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176139	.	sequence_feature	517	522	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176139	.	sequence_feature	622	714	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176139	.	non_covalent_binding_site	660	676	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	non_covalent_binding_site	679	695	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	non_covalent_binding_site	622	638	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	non_covalent_binding_site	641	657	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	non_covalent_binding_site	698	714	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	sequence_feature	616	621	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176139	.	sequence_feature	523	615	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176139	.	non_covalent_binding_site	561	577	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	non_covalent_binding_site	580	596	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	non_covalent_binding_site	523	539	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	non_covalent_binding_site	542	558	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	non_covalent_binding_site	599	615	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176139	.	engineered_region	721	2401	.	+	0	ID=BBa_S04739;Name=BBa_S04739;Parent=BBa_J176091
BBa_J176139	.	sequence_feature	2171	2176	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04739
BBa_J176139	.	engineered_region	721	2170	.	+	0	ID=BBa_S04738;Name=BBa_S04738;Parent=BBa_S04739
BBa_J176139	.	sequence_feature	739	744	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04738
BBa_J176139	.	ribosome_entry_site	721	738	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04738
BBa_J176139	.	engineered_region	745	2170	.	+	0	ID=BBa_S04684;Name=BBa_S04684;Parent=BBa_S04738
BBa_J176139	.	sequence_feature	2152	2157	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176139	.	tag	2131	2151	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04684
BBa_J176139	.	polypeptide_domain	745	1431	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176139	.	sequence_feature	1432	1437	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176139	.	polypeptide_domain	1438	2124	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176139	.	sequence_feature	2125	2130	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176139	.	terminator	2158	2170	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04684
BBa_J176139	.	tag	2177	2401	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04739
BBa_J176139	.	sequence_feature	715	720	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176091
>BBa_J176139
ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctac
acaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcg
cgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatgga
agtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggc
caatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggc
tcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct
gttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtc
ctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacggagtactgt
cctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcct
ccgactagacccgccgccaccatggagactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacct
accacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcaccca
gacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatg
tacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcct
acgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcga
gcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccc
tccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggca
actacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccg
catcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtg
gtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcg
tgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggt
gaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttc
accgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacct
acgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttcca
cggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgacc
gtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttcc
acacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacct
ggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaccc
aagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgtgccttctagttgccagccatctgttgtttgcc
cctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgca
ttgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagc
aggcatgctggggatgcggtgggctctatgggtgacgccggtgacgccgactagagtgacgccggtgacgccgctaccg
ggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagt
ggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccac
cttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagc
acgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatag
cagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggg
gcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctc
ctcttcctcatctccgggcctttcgacctggagcccactagacccgccgccaccatggagactagaatgaagctactgt
cttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtg
tctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtg
gaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatgg
attctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatag
attggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagt
agtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagagcatccaacttgaacatttcga
gaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatc
tcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatca
ccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtc
tggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatat
tgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccaccc
aagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcat
ctttaatttcttttggtatgggcaatacccaagtaataactagagtgagcaagggcgaggaggataacatggccatcat
caaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgag
ggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcc
tgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtcctt
ccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttg
caggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaaga
ccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaa
gctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcc
tacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagg
gccgccactccaccggcggcatggacgagctgtacaagactagagtgagcaagggcgaggaggataacatggccatcat
caaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgag
ggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcc
tgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtcctt
ccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttg
caggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaaga
ccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaa
gctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcc
tacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagg
gccgccactccaccggcggcatggacgagctgtacaagactagacccaagaaaaagcgcaaggtaactagagcgtaaag
ctgagactagagaatcagctatttactgccagaatcagctatttactgccagaatcagctatttactgccaactagaat
agttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagca
aatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacc
tgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgt
gccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagt
aggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctg
gggatgcggtgggctctatgg