##gff-version 3
##sequence-region BBa_J176140 1 6270
BBa_J176140	.	sequence_feature	2402	2443	.	+	0	ID=BBa_J176039;Name=BBa_J176039
BBa_J176140	.	engineered_region	2444	6270	.	+	0	ID=BBa_J176137;Name=BBa_J176137
BBa_J176140	.	sequence_feature	3020	3061	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	sequence_feature	5654	5695	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	ribosome_entry_site	3002	3019	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_J176137
BBa_J176140	.	polypeptide_domain	4139	4843	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_J176137
BBa_J176140	.	promoter	2444	2959	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_J176137
BBa_J176140	.	sequence_alteration	2954	2954	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176140	.	sequence_alteration	2696	2696	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176140	.	sequence_feature	5709	5750	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	sequence_feature	5811	5852	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	polypeptide_domain	4886	5590	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_J176137
BBa_J176140	.	sequence_feature	5591	5632	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	sequence_feature	4097	4138	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	tag	5633	5653	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_J176137
BBa_J176140	.	sequence_feature	4844	4885	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	tag	3563	4096	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_J176137
BBa_J176140	.	mature_transcript_region	5751	5810	.	+	0	ID=BBa_J176134;Name=BBa_J176134;Parent=BBa_J176137
BBa_J176140	.	non_covalent_binding_site	5771	5790	.	+	0	ID=annotation2166751;Name=CCND1 miR-34 target;Parent=BBa_J176134
BBa_J176140	.	non_covalent_binding_site	5791	5810	.	+	0	ID=annotation2166752;Name=CCND1 miR-34 target;Parent=BBa_J176134
BBa_J176140	.	non_covalent_binding_site	5751	5770	.	+	0	ID=annotation2166750;Name=CCND1 miR-34 target;Parent=BBa_J176134
BBa_J176140	.	polypeptide_domain	3062	3520	.	+	0	ID=BBa_J176020;Name=BBa_J176020;Parent=BBa_J176137
BBa_J176140	.	sequence_feature	2960	3001	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	engineered_region	5853	6270	.	+	0	ID=BBa_S04730;Name=BBa_S04730;Parent=BBa_J176137
BBa_J176140	.	sequence_feature	6040	6045	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04730
BBa_J176140	.	tag	6046	6270	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04730
BBa_J176140	.	mature_transcript_region	5853	6039	.	+	0	ID=BBa_J176025;Name=BBa_J176025;Parent=BBa_S04730
BBa_J176140	.	sequence_feature	5907	5925	.	+	0	ID=annotation2166808;Name=repeat 2;Parent=BBa_J176025
BBa_J176140	.	sequence_feature	5934	5952	.	+	0	ID=annotation2166809;Name=repeat 3;Parent=BBa_J176025
BBa_J176140	.	sequence_feature	5987	6005	.	+	0	ID=annotation2166811;Name=repeat 5;Parent=BBa_J176025
BBa_J176140	.	sequence_feature	5880	5898	.	+	0	ID=annotation2166807;Name=repeat 1;Parent=BBa_J176025
BBa_J176140	.	sequence_feature	5961	5979	.	+	0	ID=annotation2166810;Name=repeat 4;Parent=BBa_J176025
BBa_J176140	.	sequence_feature	3521	3562	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176137
BBa_J176140	.	terminator	5696	5708	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_J176137
BBa_J176140	.	engineered_region	1	2401	.	+	0	ID=BBa_J176091;Name=BBa_J176091
BBa_J176140	.	sequence_feature	715	720	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176091
BBa_J176140	.	engineered_region	721	2401	.	+	0	ID=BBa_S04739;Name=BBa_S04739;Parent=BBa_J176091
BBa_J176140	.	engineered_region	721	2170	.	+	0	ID=BBa_S04738;Name=BBa_S04738;Parent=BBa_S04739
BBa_J176140	.	sequence_feature	739	744	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04738
BBa_J176140	.	engineered_region	745	2170	.	+	0	ID=BBa_S04684;Name=BBa_S04684;Parent=BBa_S04738
BBa_J176140	.	sequence_feature	2152	2157	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176140	.	tag	2131	2151	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04684
BBa_J176140	.	polypeptide_domain	745	1431	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176140	.	sequence_feature	1432	1437	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176140	.	polypeptide_domain	1438	2124	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176140	.	sequence_feature	2125	2130	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176140	.	terminator	2158	2170	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04684
BBa_J176140	.	ribosome_entry_site	721	738	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04738
BBa_J176140	.	tag	2177	2401	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04739
BBa_J176140	.	sequence_feature	2171	2176	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04739
BBa_J176140	.	engineered_region	1	714	.	+	0	ID=BBa_S04655;Name=BBa_S04655;Parent=BBa_J176091
BBa_J176140	.	sequence_feature	622	714	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176140	.	non_covalent_binding_site	660	676	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	non_covalent_binding_site	622	638	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	non_covalent_binding_site	679	695	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	non_covalent_binding_site	641	657	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	non_covalent_binding_site	698	714	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	sequence_feature	616	621	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176140	.	sequence_feature	517	522	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176140	.	sequence_feature	523	615	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176140	.	non_covalent_binding_site	561	577	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	non_covalent_binding_site	523	539	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	non_covalent_binding_site	580	596	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	non_covalent_binding_site	542	558	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	non_covalent_binding_site	599	615	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176140	.	promoter	1	516	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04655
BBa_J176140	.	sequence_alteration	511	511	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176140	.	sequence_alteration	253	253	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
>BBa_J176140
ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctac
acaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcg
cgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatgga
agtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggc
caatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggc
tcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct
gttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtc
ctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacggagtactgt
cctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcct
ccgactagacccgccgccaccatggagactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacct
accacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcaccca
gacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatg
tacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcct
acgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcga
gcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccc
tccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggca
actacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccg
catcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtg
gtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcg
tgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggt
gaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttc
accgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacct
acgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttcca
cggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgacc
gtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttcc
acacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacct
ggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaccc
aagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgtgccttctagttgccagccatctgttgtttgcc
cctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgca
ttgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagc
aggcatgctggggatgcggtgggctctatgggtgacgccggtgacgccgactagagtgacgccggtgacgccgctaccg
ggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagt
ggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccac
cttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagc
acgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatag
cagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggg
gcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctc
ctcttcctcatctccgggcctttcgacctggagcccgtgacgccggtgacgccgactagagtgacgccggtgacgccgc
ccgccgccaccatggaggtgacgccggtgacgccgactagagtgacgccggtgacgccgatgaagctactgtcttctat
cgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaag
aacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaa
ggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattcttt
acaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggct
tcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaaca
aaggtcaaagacagttgactgtatcgccggaatttccggggatcgtgacgccggtgacgccgactagagtgacgccggt
gacgccggcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagc
acatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcg
ttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtat
atgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcct
aacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaa
cggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccat
aaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaatagtgacgccggtg
acgccgactagagtgacgccggtgacgccggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgc
gcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacga
gggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttc
atgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttca
agtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagtt
catctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggag
gcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcg
gccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacat
caagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccacc
ggcggcatggacgagctgtacaaggtgacgccggtgacgccgactagagtgacgccggtgacgccggtgagcaagggcg
aggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagtt
cgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggcccc
ctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatcc
ccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgac
cgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgac
ggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagg
gcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaa
gcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtg
gaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggtgacgccggtgacgccga
ctagagtgacgccggtgacgccgcccaagaaaaagcgcaaggtagtgacgccggtgacgccgactagagtgacgccggt
gacgccggcgtaaagctgaggtgacgccggtgacgccgactagagtgacgccggtgacgccgacaatgtcatatactgc
catacaatgtcatatactgccatacaatgtcatatactgccatgtgacgccggtgacgccgactagagtgacgccggtg
acgccgatagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaa
gtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataa
agtcgacctgcaagcatgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccc
tcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcatt
gtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcag
gcatgctggggatgcggtgggctctatgg