LOCUS BBa_J176141 6412 bp DNA linear 22-Dec-2024 DEFINITION miR-34 CDK6 sensor ACCESSION BBa_J176141 VERSION BBa_J176141.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 616..621 /label=BBa_J176041 misc_feature 622..638 /label=Gal4 misc_feature 641..657 /label=Gal4 misc_feature 660..676 /label=Gal4 misc_feature 679..695 /label=Gal4 misc_feature 698..714 /label=Gal4 misc_feature 622..714 /label=BBa_J176019 misc_feature 1..714 /label=BBa_S04655 misc_feature 715..720 /label=BBa_J176041 RBS 721..738 /label=BBa_J176012 misc_feature 739..744 /label=BBa_J176041 misc_feature 745..1431 /label=BBa_J176022 misc_feature 1432..1437 /label=BBa_J176041 misc_feature 1438..2124 /label=BBa_J176022 misc_feature 2125..2130 /label=BBa_J176041 misc_feature 2131..2151 /label=BBa_J176008 misc_feature 2152..2157 /label=BBa_J176041 terminator 2158..2170 /label=BBa_J176016 misc_feature 745..2170 /label=BBa_S04684 misc_feature 721..2170 /label=BBa_S04738 misc_feature 2171..2176 /label=BBa_J176041 misc_feature 2177..2401 /label=BBa_J176009 misc_feature 721..2401 /label=BBa_S04739 misc_feature 1..2401 /label=BBa_J176091 misc_feature 2402..2443 /label=BBa_J176039 misc_feature 2696..2696 /label=mutated SpeI misc_feature 2954..2954 /label=mutated PstI promoter 2444..2959 /label=BBa_J176028 misc_feature 2968..3009 /label=BBa_J176039 RBS 3018..3035 /label=BBa_J176012 misc_feature 3044..3085 /label=BBa_J176039 misc_feature 3092..3550 /label=BBa_J176020 misc_feature 3559..3600 /label=BBa_J176039 misc_feature 3609..4142 /label=BBa_J176018 misc_feature 4151..4192 /label=BBa_J176039 misc_feature 4201..4905 /label=BBa_J176005 misc_feature 4914..4955 /label=BBa_J176039 misc_feature 4964..5668 /label=BBa_J176005 misc_feature 5677..5718 /label=BBa_J176039 misc_feature 5727..5747 /label=BBa_J176008 misc_feature 5756..5797 /label=BBa_J176039 terminator 5806..5818 /label=BBa_J176016 misc_feature 5827..5868 /label=BBa_J176039 misc_feature 5877..5896 /label=CDK6 miR-34 target misc_feature 5897..5916 /label=CDK6 miR-34 target misc_feature 5917..5936 /label=CDK6 miR-34 target misc_feature 5877..5936 /label=BBa_J176135 misc_feature 5945..5986 /label=BBa_J176039 misc_feature 6022..6040 /label=repeat 1 misc_feature 6049..6067 /label=repeat 2 misc_feature 6076..6094 /label=repeat 3 misc_feature 6103..6121 /label=repeat 4 misc_feature 6129..6147 /label=repeat 5 misc_feature 5995..6181 /label=BBa_J176025 misc_feature 6182..6187 /label=BBa_J176041 misc_feature 6188..6412 /label=BBa_J176009 misc_feature 5995..6412 /label=BBa_S04730 misc_feature 2444..6412 /label=BBa_J176138 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acggagtact gtcctccgag cggagtactg tcctccgagc 661 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgactaga 721 cccgccgcca ccatggagac tagaatggcc ctgtccaaca agttcatcgg cgacgacatg 781 aagatgacct accacatgga cggctgcgtg aacggccact acttcaccgt gaagggcgag 841 ggcagcggca agccctacga gggcacccag acctccacct tcaaggtgac catggccaac 901 ggcggccccc tggccttctc cttcgacatc ctgtccaccg tgttcatgta cggcaaccgc 961 tgcttcaccg cctaccccac cagcatgccc gactacttca agcaggcctt ccccgacggc 1021 atgtcctacg agagaacctt cacctacgag gacggcggcg tggccaccgc cagctgggag 1081 atcagcctga agggcaactg cttcgagcac aagtccacct tccacggcgt gaacttcccc 1141 gccgacggcc ccgtgatggc caagaagacc accggctggg acccctcctt cgagaagatg 1201 accgtgtgcg acggcatctt gaagggcgac gtgaccgcct tcctgatgct gcaaggcggc 1261 ggcaactaca gatgccagtt ccacacctcc tacaagacca agaagcccgt gaccatgccc 1321 cccaaccacg tggtggagca ccgcatcgcc agaaccgacc tggacaaggg cggcaacagc 1381 gtgcagctga ccgagcacgc cgtggcccac atcacctccg tggtgccctt cactagaatg 1441 gccctgtcca acaagttcat cggcgacgac atgaagatga cctaccacat ggacggctgc 1501 gtgaacggcc actacttcac cgtgaagggc gagggcagcg gcaagcccta cgagggcacc 1561 cagacctcca ccttcaaggt gaccatggcc aacggcggcc ccctggcctt ctccttcgac 1621 atcctgtcca ccgtgttcat gtacggcaac cgctgcttca ccgcctaccc caccagcatg 1681 cccgactact tcaagcaggc cttccccgac ggcatgtcct acgagagaac cttcacctac 1741 gaggacggcg gcgtggccac cgccagctgg gagatcagcc tgaagggcaa ctgcttcgag 1801 cacaagtcca ccttccacgg cgtgaacttc cccgccgacg gccccgtgat ggccaagaag 1861 accaccggct gggacccctc cttcgagaag atgaccgtgt gcgacggcat cttgaagggc 1921 gacgtgaccg ccttcctgat gctgcaaggc ggcggcaact acagatgcca gttccacacc 1981 tcctacaaga ccaagaagcc cgtgaccatg ccccccaacc acgtggtgga gcaccgcatc 2041 gccagaaccg acctggacaa gggcggcaac agcgtgcagc tgaccgagca cgccgtggcc 2101 cacatcacct ccgtggtgcc cttcactaga cccaagaaaa agcgcaaggt aactagagcg 2161 taaagctgag actagactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 2221 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 2281 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 2341 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 2401 ggtgacgccg gtgacgccga ctagagtgac gccggtgacg ccgctaccgg gtaggggagg 2461 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct 2521 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc 2581 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc 2641 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcacaagtc 2701 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc 2761 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg 2821 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg 2881 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 2941 gcctttcgac ctggagccct actagaggtg acgccggtga cgccgactag agtgacgccg 3001 gtgacgccgt actagagccc gccgccacca tggagtacta gaggtgacgc cggtgacgcc 3061 gactagagtg acgccggtga cgccgtacta gatgaagcta ctgtcttcta tcgaacaagc 3121 atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa 3181 gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac 3241 tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact 3301 gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac aggatataaa 3361 agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg tcacagatag 3421 attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa taagtgcgac 3481 atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat cgccggaatt 3541 tccggggatc tactagaggt gacgccggtg acgccgacta gagtgacgcc ggtgacgccg 3601 tactagaggc atccaacttg aacatttcga gaaagcttac catatcaacc ccatcatgct 3661 ctttcgaaaa ttcaaatagc acatccattc cttcgcccgc ttcctcatct caaagccaca 3721 ctccaatgag aaacatgagc tcactctctg ataacagcgt tttcagccgg aatatggaac 3781 aatcatcacc aatcactcca agtatgtacc aatttggtca gcagcagtca aacagtatat 3841 gtggtagcac cgttagtgtg aatagtctgg tgaatacaaa taacaaacaa aggatctacg 3901 aacaaatcac gggtcctaac agcaataacg caaccaatga ttatattgat ttgctaaacc 3961 taaatgagtc taacaaggaa aaccaaaatc ccgcaacggc gcattacctc aatgggggcc 4021 cacccaagac aagcttcatt aaccatggaa tgttcccctc gccaactggg accataaata 4081 gcggtaaatc tagcagtgcc tcatctttaa tttcttttgg tatgggcaat acccaagtaa 4141 tatactagag gtgacgccgg tgacgccgac tagagtgacg ccggtgacgc cgtactagag 4201 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg 4261 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 4321 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc 4381 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc 4441 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 4501 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc 4561 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg 4621 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc 4681 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag 4741 gtcaagacca cctacaaggc caagaagccc gtgcagctac ccggcgccta caacgtcaac 4801 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc 4861 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtacta gaggtgacgc 4921 cggtgacgcc gactagagtg acgccggtga cgccgtacta gaggtgagca agggcgagga 4981 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt 5041 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca 5101 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc 5161 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta 5221 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg 5281 cggcgtggtg accgtgaccc aggactcctc cttgcaggac ggcgagttca tctacaaggt 5341 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg 5401 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa 5461 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa 5521 ggccaagaag cccgtgcagc tacccggcgc ctacaacgtc aacatcaagt tggacatcac 5581 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc 5641 caccggcggc atggacgagc tgtacaagta ctagaggtga cgccggtgac gccgactaga 5701 gtgacgccgg tgacgccgta ctagagccca agaaaaagcg caaggtatac tagaggtgac 5761 gccggtgacg ccgactagag tgacgccggt gacgccgtac tagaggcgta aagctgagta 5821 ctagaggtga cgccggtgac gccgactaga gtgacgccgg tgacgccgta ctagagcagt 5881 gtggaaattc actgcccagt gtggaaattc actgcccagt gtggaaattc actgcctact 5941 agaggtgacg ccggtgacgc cgactagagt gacgccggtg acgccgtact agagatagtt 6001 gtttaaacga gctcgctagc aaatcaagag tcctcataaa ggaattaaaa tcaagagtcc 6061 tcataaagtc tagcaaatca agagtcctca taaagctaga caaatcaaga gtcctcataa 6121 ataattccaa tcaagagtcc tcataaagtc gacctgcaag catgcaagct gatccggctg 6181 cactagactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 6241 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 6301 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 6361 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat gg //