##gff-version 3
##sequence-region BBa_J176141 1 6412
BBa_J176141	.	sequence_feature	2402	2443	.	+	0	ID=BBa_J176039;Name=BBa_J176039
BBa_J176141	.	engineered_region	2444	6412	.	+	0	ID=BBa_J176138;Name=BBa_J176138
BBa_J176141	.	promoter	2444	2959	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_J176138
BBa_J176141	.	sequence_alteration	2954	2954	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176141	.	sequence_alteration	2696	2696	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176141	.	sequence_feature	5945	5986	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	sequence_feature	5827	5868	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	polypeptide_domain	4201	4905	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_J176138
BBa_J176141	.	sequence_feature	2968	3009	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	sequence_feature	5677	5718	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	polypeptide_domain	4964	5668	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_J176138
BBa_J176141	.	polypeptide_domain	3092	3550	.	+	0	ID=BBa_J176020;Name=BBa_J176020;Parent=BBa_J176138
BBa_J176141	.	tag	5727	5747	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_J176138
BBa_J176141	.	engineered_region	5995	6412	.	+	0	ID=BBa_S04730;Name=BBa_S04730;Parent=BBa_J176138
BBa_J176141	.	sequence_feature	6182	6187	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04730
BBa_J176141	.	mature_transcript_region	5995	6181	.	+	0	ID=BBa_J176025;Name=BBa_J176025;Parent=BBa_S04730
BBa_J176141	.	sequence_feature	6049	6067	.	+	0	ID=annotation2166808;Name=repeat 2;Parent=BBa_J176025
BBa_J176141	.	sequence_feature	6076	6094	.	+	0	ID=annotation2166809;Name=repeat 3;Parent=BBa_J176025
BBa_J176141	.	sequence_feature	6129	6147	.	+	0	ID=annotation2166811;Name=repeat 5;Parent=BBa_J176025
BBa_J176141	.	sequence_feature	6103	6121	.	+	0	ID=annotation2166810;Name=repeat 4;Parent=BBa_J176025
BBa_J176141	.	sequence_feature	6022	6040	.	+	0	ID=annotation2166807;Name=repeat 1;Parent=BBa_J176025
BBa_J176141	.	tag	6188	6412	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04730
BBa_J176141	.	sequence_feature	5756	5797	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	sequence_feature	4151	4192	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	mature_transcript_region	5877	5936	.	+	0	ID=BBa_J176135;Name=BBa_J176135;Parent=BBa_J176138
BBa_J176141	.	non_covalent_binding_site	5877	5896	.	+	0	ID=annotation2166753;Name=CDK6 miR-34 target;Parent=BBa_J176135
BBa_J176141	.	non_covalent_binding_site	5897	5916	.	+	0	ID=annotation2166754;Name=CDK6 miR-34 target;Parent=BBa_J176135
BBa_J176141	.	non_covalent_binding_site	5917	5936	.	+	0	ID=annotation2166755;Name=CDK6 miR-34 target;Parent=BBa_J176135
BBa_J176141	.	sequence_feature	4914	4955	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	terminator	5806	5818	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_J176138
BBa_J176141	.	sequence_feature	3559	3600	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	tag	3609	4142	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_J176138
BBa_J176141	.	sequence_feature	3044	3085	.	+	0	ID=BBa_J176039;Name=BBa_J176039;Parent=BBa_J176138
BBa_J176141	.	ribosome_entry_site	3018	3035	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_J176138
BBa_J176141	.	engineered_region	1	2401	.	+	0	ID=BBa_J176091;Name=BBa_J176091
BBa_J176141	.	engineered_region	721	2401	.	+	0	ID=BBa_S04739;Name=BBa_S04739;Parent=BBa_J176091
BBa_J176141	.	sequence_feature	2171	2176	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04739
BBa_J176141	.	engineered_region	721	2170	.	+	0	ID=BBa_S04738;Name=BBa_S04738;Parent=BBa_S04739
BBa_J176141	.	ribosome_entry_site	721	738	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04738
BBa_J176141	.	sequence_feature	739	744	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04738
BBa_J176141	.	engineered_region	745	2170	.	+	0	ID=BBa_S04684;Name=BBa_S04684;Parent=BBa_S04738
BBa_J176141	.	sequence_feature	2152	2157	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176141	.	tag	2131	2151	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04684
BBa_J176141	.	polypeptide_domain	745	1431	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176141	.	sequence_feature	1432	1437	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176141	.	polypeptide_domain	1438	2124	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176141	.	sequence_feature	2125	2130	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176141	.	terminator	2158	2170	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04684
BBa_J176141	.	tag	2177	2401	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04739
BBa_J176141	.	engineered_region	1	714	.	+	0	ID=BBa_S04655;Name=BBa_S04655;Parent=BBa_J176091
BBa_J176141	.	sequence_feature	622	714	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176141	.	non_covalent_binding_site	641	657	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	non_covalent_binding_site	622	638	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	non_covalent_binding_site	660	676	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	non_covalent_binding_site	698	714	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	non_covalent_binding_site	679	695	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	sequence_feature	517	522	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176141	.	promoter	1	516	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04655
BBa_J176141	.	sequence_alteration	511	511	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176141	.	sequence_alteration	253	253	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176141	.	sequence_feature	616	621	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176141	.	sequence_feature	523	615	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176141	.	non_covalent_binding_site	542	558	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	non_covalent_binding_site	523	539	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	non_covalent_binding_site	561	577	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	non_covalent_binding_site	599	615	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	non_covalent_binding_site	580	596	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176141	.	sequence_feature	715	720	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176091
>BBa_J176141
ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctac
acaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcg
cgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatgga
agtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggc
caatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggc
tcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct
gttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtc
ctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacggagtactgt
cctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcct
ccgactagacccgccgccaccatggagactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacct
accacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcaccca
gacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatg
tacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcct
acgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcga
gcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccc
tccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggca
actacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccg
catcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtg
gtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcg
tgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggt
gaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttc
accgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacct
acgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttcca
cggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgacc
gtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttcc
acacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacct
ggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaccc
aagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgtgccttctagttgccagccatctgttgtttgcc
cctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgca
ttgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagc
aggcatgctggggatgcggtgggctctatgggtgacgccggtgacgccgactagagtgacgccggtgacgccgctaccg
ggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagt
ggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccac
cttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagc
acgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatag
cagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggg
gcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctc
ctcttcctcatctccgggcctttcgacctggagccctactagaggtgacgccggtgacgccgactagagtgacgccggt
gacgccgtactagagcccgccgccaccatggagtactagaggtgacgccggtgacgccgactagagtgacgccggtgac
gccgtactagatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaa
gaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctga
ctagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaaga
ccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtg
aataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtg
cgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatctacta
gaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagaggcatccaacttgaacatttcgagaaa
gcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaa
agccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaa
tcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggt
gaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgat
ttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaaga
caagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatcttt
aatttcttttggtatgggcaatacccaagtaatatactagaggtgacgccggtgacgccgactagagtgacgccggtga
cgccgtactagaggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatg
gagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgcca
agctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggc
ctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatg
aacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagc
tgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggat
gtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgag
gtcaagaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacct
cccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagct
gtacaagtactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagaggtgagcaagggcga
ggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttc
gagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccc
tgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccc
cgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgacc
gtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacg
gccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaaggg
cgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaag
cccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtgg
aacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtactagaggtgacgccggtg
acgccgactagagtgacgccggtgacgccgtactagagcccaagaaaaagcgcaaggtatactagaggtgacgccggtg
acgccgactagagtgacgccggtgacgccgtactagaggcgtaaagctgagtactagaggtgacgccggtgacgccgac
tagagtgacgccggtgacgccgtactagagcagtgtggaaattcactgcccagtgtggaaattcactgcccagtgtgga
aattcactgcctactagaggtgacgccggtgacgccgactagagtgacgccggtgacgccgtactagagatagttgttt
aaacgagctcgctagcaaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaaga
gtcctcataaagctagacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagca
tgcaagctgatccggctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttcct
tgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtca
ttctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcg
gtgggctctatgg