LOCUS BBa_J176143 5498 bp DNA linear 25-Nov-2024 DEFINITION Test miR-luc sensor 2 ACCESSION BBa_J176143 VERSION BBa_J176143.1 FEATURES Location/Qualifiers promoter 1..368 /label=BBa_J176003 misc_feature 369..374 /label=BBa_J176041 RBS 375..392 /label=BBa_J176012 misc_feature 393..398 /label=BBa_J176041 misc_feature 399..1085 /label=BBa_J176022 misc_feature 1086..1091 /label=BBa_J176041 misc_feature 1092..1778 /label=BBa_J176022 misc_feature 1779..1784 /label=BBa_J176041 misc_feature 1785..1805 /label=BBa_J176008 misc_feature 1806..1811 /label=BBa_J176041 terminator 1812..1824 /label=BBa_J176016 misc_feature 399..1824 /label=BBa_S04684 misc_feature 375..1824 /label=BBa_S04738 misc_feature 1825..1830 /label=BBa_J176041 misc_feature 1831..2055 /label=BBa_J176009 misc_feature 375..2055 /label=BBa_S04739 misc_feature 1..2055 /label=BBa_J176093 misc_feature 2056..2061 /label=BBa_J176041 misc_feature 2314..2314 /label=mutated SpeI misc_feature 2572..2572 /label=mutated PstI promoter 2062..2577 /label=BBa_J176028 misc_feature 2578..2583 /label=BBa_J176041 RBS 2584..2601 /label=BBa_J176012 misc_feature 2602..2607 /label=BBa_J176041 misc_feature 2608..3066 /label=BBa_J176020 misc_feature 3067..3072 /label=BBa_J176041 misc_feature 3073..3606 /label=BBa_J176018 misc_feature 3607..3612 /label=BBa_J176041 misc_feature 3613..4317 /label=BBa_J176005 misc_feature 4318..4323 /label=BBa_J176041 misc_feature 4324..5028 /label=BBa_J176005 misc_feature 5029..5034 /label=BBa_J176041 misc_feature 5035..5055 /label=BBa_J176008 misc_feature 5056..5061 /label=BBa_J176041 terminator 5062..5074 /label=BBa_J176016 misc_feature 2062..5074 /label=BBa_S04732 misc_feature 5075..5080 /label=BBa_J176041 misc_feature 5108..5126 /label=repeat 1 misc_feature 5135..5153 /label=repeat 2 misc_feature 5162..5180 /label=repeat 3 misc_feature 5189..5207 /label=repeat 4 misc_feature 5215..5233 /label=repeat 5 misc_feature 5081..5267 /label=BBa_J176025 misc_feature 5268..5273 /label=BBa_J176041 misc_feature 5274..5498 /label=BBa_J176009 misc_feature 5081..5498 /label=BBa_S04730 misc_feature 2062..5498 /label=BBa_S04736 ORIGIN 1 aaactggcct ccgcgccggg cttttggcgc ctcccgcggg cgcccccctc ctcacggcga 61 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca 121 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca 181 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag 241 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac 301 gccgatgatt atataaggac gcgccgggtg tggcacagct agttactaga cccgccgcca 361 ccatggagac tagacccgcc gccaccatgg agactagaat ggccctgtcc aacaagttca 421 tcggcgacga catgaagatg acctaccaca tggacggctg cgtgaacggc cactacttca 481 ccgtgaaggg cgagggcagc ggcaagccct acgagggcac ccagacctcc accttcaagg 541 tgaccatggc caacggcggc cccctggcct tctccttcga catcctgtcc accgtgttca 601 tgtacggcaa ccgctgcttc accgcctacc ccaccagcat gcccgactac ttcaagcagg 661 ccttccccga cggcatgtcc tacgagagaa ccttcaccta cgaggacggc ggcgtggcca 721 ccgccagctg ggagatcagc ctgaagggca actgcttcga gcacaagtcc accttccacg 781 gcgtgaactt ccccgccgac ggccccgtga tggccaagaa gaccaccggc tgggacccct 841 ccttcgagaa gatgaccgtg tgcgacggca tcttgaaggg cgacgtgacc gccttcctga 901 tgctgcaagg cggcggcaac tacagatgcc agttccacac ctcctacaag accaagaagc 961 ccgtgaccat gccccccaac cacgtggtgg agcaccgcat cgccagaacc gacctggaca 1021 agggcggcaa cagcgtgcag ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc 1081 ccttcactag aatggccctg tccaacaagt tcatcggcga cgacatgaag atgacctacc 1141 acatggacgg ctgcgtgaac ggccactact tcaccgtgaa gggcgagggc agcggcaagc 1201 cctacgaggg cacccagacc tccaccttca aggtgaccat ggccaacggc ggccccctgg 1261 ccttctcctt cgacatcctg tccaccgtgt tcatgtacgg caaccgctgc ttcaccgcct 1321 accccaccag catgcccgac tacttcaagc aggccttccc cgacggcatg tcctacgaga 1381 gaaccttcac ctacgaggac ggcggcgtgg ccaccgccag ctgggagatc agcctgaagg 1441 gcaactgctt cgagcacaag tccaccttcc acggcgtgaa cttccccgcc gacggccccg 1501 tgatggccaa gaagaccacc ggctgggacc cctccttcga gaagatgacc gtgtgcgacg 1561 gcatcttgaa gggcgacgtg accgccttcc tgatgctgca aggcggcggc aactacagat 1621 gccagttcca cacctcctac aagaccaaga agcccgtgac catgcccccc aaccacgtgg 1681 tggagcaccg catcgccaga accgacctgg acaagggcgg caacagcgtg cagctgaccg 1741 agcacgccgt ggcccacatc acctccgtgg tgcccttcac tagacccaag aaaaagcgca 1801 aggtaactag agcgtaaagc tgagactaga ctgtgccttc tagttgccag ccatctgttg 1861 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 1921 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 1981 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 2041 cggtgggctc tatggactag actaccgggt aggggaggcg cttttcccaa ggcagtctgg 2101 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg 2161 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc 2221 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg 2281 caggacgtga caaatggaag tagcacgtct cacaagtctc gtgcagatgg acagcaccgc 2341 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc 2401 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag 2461 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg 2521 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct ggagcccact 2581 agacccgccg ccaccatgga gactagaatg aagctactgt cttctatcga acaagcatgc 2641 gatatttgcc gacttaaaaa gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt 2701 ctgaagaaca actgggagtg tcgctactct cccaaaacca aaaggtctcc gctgactagg 2761 gcacatctga cagaagtgga atcaaggcta gaaagactgg aacagctatt tctactgatt 2821 tttcctcgag aagaccttga catgattttg aaaatggatt ctttacagga tataaaagca 2881 ttgttaacag gattatttgt acaagataat gtgaataaag atgccgtcac agatagattg 2941 gcttcagtgg agactgatat gcctctaaca ttgagacagc atagaataag tgcgacatca 3001 tcatcggaag agagtagtaa caaaggtcaa agacagttga ctgtatcgcc ggaatttccg 3061 gggatcacta gagcatccaa cttgaacatt tcgagaaagc ttaccatatc aaccccatca 3121 tgctctttcg aaaattcaaa tagcacatcc attccttcgc ccgcttcctc atctcaaagc 3181 cacactccaa tgagaaacat gagctcactc tctgataaca gcgttttcag ccggaatatg 3241 gaacaatcat caccaatcac tccaagtatg taccaatttg gtcagcagca gtcaaacagt 3301 atatgtggta gcaccgttag tgtgaatagt ctggtgaata caaataacaa acaaaggatc 3361 tacgaacaaa tcacgggtcc taacagcaat aacgcaacca atgattatat tgatttgcta 3421 aacctaaatg agtctaacaa ggaaaaccaa aatcccgcaa cggcgcatta cctcaatggg 3481 ggcccaccca agacaagctt cattaaccat ggaatgttcc cctcgccaac tgggaccata 3541 aatagcggta aatctagcag tgcctcatct ttaatttctt ttggtatggg caatacccaa 3601 gtaataacta gagtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg 3661 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag 3721 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc 3781 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac 3841 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag 3901 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc 3961 ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac 4021 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc 4081 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac 4141 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct acccggcgcc 4201 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa 4261 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagact 4321 agagtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag 4381 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc 4441 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc 4501 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac 4561 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc 4621 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cttgcaggac 4681 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta 4741 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc 4801 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct 4861 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tacccggcgc ctacaacgtc 4921 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa 4981 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagac tagacccaag 5041 aaaaagcgca aggtaactag agcgtaaagc tgagactaga atagttgttt aaacgagctc 5101 gctagcaaat caagagtcct cataaaggaa ttaaaatcaa gagtcctcat aaagtctagc 5161 aaatcaagag tcctcataaa gctagacaaa tcaagagtcc tcataaataa ttccaatcaa 5221 gagtcctcat aaagtcgacc tgcaagcatg caagctgatc cggctgcact agactgtgcc 5281 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 5341 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 5401 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 5461 caatagcagg catgctgggg atgcggtggg ctctatgg //