LOCUS BBa_J176146 5613 bp DNA linear 22-Nov-2024 DEFINITION Test miR-luc sensor 5 ACCESSION BBa_J176146 VERSION BBa_J176146.1 FEATURES Location/Qualifiers promoter 1..368 /label=BBa_J176003 misc_feature 369..374 /label=BBa_J176041 misc_feature 375..391 /label=Gal4 misc_feature 394..410 /label=Gal4 misc_feature 413..429 /label=Gal4 misc_feature 432..448 /label=Gal4 misc_feature 451..467 /label=Gal4 misc_feature 375..467 /label=BBa_J176019 misc_feature 1..467 /label=BBa_S04642 misc_feature 468..473 /label=BBa_J176041 RBS 474..491 /label=BBa_J176012 misc_feature 492..497 /label=BBa_J176041 misc_feature 498..1184 /label=BBa_J176022 misc_feature 1185..1190 /label=BBa_J176041 misc_feature 1191..1877 /label=BBa_J176022 misc_feature 1878..1883 /label=BBa_J176041 misc_feature 1884..1904 /label=BBa_J176008 misc_feature 1905..1910 /label=BBa_J176041 terminator 1911..1923 /label=BBa_J176016 misc_feature 498..1923 /label=BBa_S04684 misc_feature 474..1923 /label=BBa_S04738 misc_feature 1924..1929 /label=BBa_J176041 misc_feature 1930..2154 /label=BBa_J176009 misc_feature 474..2154 /label=BBa_S04739 misc_feature 1..2154 /label=BBa_J176095 misc_feature 2163..2168 /label=BBa_J176041 misc_feature 2429..2429 /label=mutated SpeI misc_feature 2687..2687 /label=mutated PstI promoter 2177..2692 /label=BBa_J176028 misc_feature 2693..2698 /label=BBa_J176041 RBS 2699..2716 /label=BBa_J176012 misc_feature 2717..2722 /label=BBa_J176041 misc_feature 2723..3181 /label=BBa_J176020 misc_feature 3182..3187 /label=BBa_J176041 misc_feature 3188..3721 /label=BBa_J176018 misc_feature 3722..3727 /label=BBa_J176041 misc_feature 3728..4432 /label=BBa_J176005 misc_feature 4433..4438 /label=BBa_J176041 misc_feature 4439..5143 /label=BBa_J176005 misc_feature 5144..5149 /label=BBa_J176041 misc_feature 5150..5170 /label=BBa_J176008 misc_feature 5171..5176 /label=BBa_J176041 terminator 5177..5189 /label=BBa_J176016 misc_feature 2177..5189 /label=BBa_S04732 misc_feature 5190..5195 /label=BBa_J176041 misc_feature 5223..5241 /label=repeat 1 misc_feature 5250..5268 /label=repeat 2 misc_feature 5277..5295 /label=repeat 3 misc_feature 5304..5322 /label=repeat 4 misc_feature 5330..5348 /label=repeat 5 misc_feature 5196..5382 /label=BBa_J176025 misc_feature 5383..5388 /label=BBa_J176041 misc_feature 5389..5613 /label=BBa_J176009 misc_feature 5196..5613 /label=BBa_S04730 misc_feature 2177..5613 /label=BBa_S04736 ORIGIN 1 aaactggcct ccgcgccggg cttttggcgc ctcccgcggg cgcccccctc ctcacggcga 61 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca 121 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca 181 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag 241 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac 301 gccgatgatt atataaggac gcgccgggtg tggcacagct agttactaga cccgccgcca 361 ccatggagac tagacggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac 421 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgact agacccgccg 481 ccaccatgga gactagaatg gccctgtcca acaagttcat cggcgacgac atgaagatga 541 cctaccacat ggacggctgc gtgaacggcc actacttcac cgtgaagggc gagggcagcg 601 gcaagcccta cgagggcacc cagacctcca ccttcaaggt gaccatggcc aacggcggcc 661 ccctggcctt ctccttcgac atcctgtcca ccgtgttcat gtacggcaac cgctgcttca 721 ccgcctaccc caccagcatg cccgactact tcaagcaggc cttccccgac ggcatgtcct 781 acgagagaac cttcacctac gaggacggcg gcgtggccac cgccagctgg gagatcagcc 841 tgaagggcaa ctgcttcgag cacaagtcca ccttccacgg cgtgaacttc cccgccgacg 901 gccccgtgat ggccaagaag accaccggct gggacccctc cttcgagaag atgaccgtgt 961 gcgacggcat cttgaagggc gacgtgaccg ccttcctgat gctgcaaggc ggcggcaact 1021 acagatgcca gttccacacc tcctacaaga ccaagaagcc cgtgaccatg ccccccaacc 1081 acgtggtgga gcaccgcatc gccagaaccg acctggacaa gggcggcaac agcgtgcagc 1141 tgaccgagca cgccgtggcc cacatcacct ccgtggtgcc cttcactaga atggccctgt 1201 ccaacaagtt catcggcgac gacatgaaga tgacctacca catggacggc tgcgtgaacg 1261 gccactactt caccgtgaag ggcgagggca gcggcaagcc ctacgagggc acccagacct 1321 ccaccttcaa ggtgaccatg gccaacggcg gccccctggc cttctccttc gacatcctgt 1381 ccaccgtgtt catgtacggc aaccgctgct tcaccgccta ccccaccagc atgcccgact 1441 acttcaagca ggccttcccc gacggcatgt cctacgagag aaccttcacc tacgaggacg 1501 gcggcgtggc caccgccagc tgggagatca gcctgaaggg caactgcttc gagcacaagt 1561 ccaccttcca cggcgtgaac ttccccgccg acggccccgt gatggccaag aagaccaccg 1621 gctgggaccc ctccttcgag aagatgaccg tgtgcgacgg catcttgaag ggcgacgtga 1681 ccgccttcct gatgctgcaa ggcggcggca actacagatg ccagttccac acctcctaca 1741 agaccaagaa gcccgtgacc atgcccccca accacgtggt ggagcaccgc atcgccagaa 1801 ccgacctgga caagggcggc aacagcgtgc agctgaccga gcacgccgtg gcccacatca 1861 cctccgtggt gcccttcact agacccaaga aaaagcgcaa ggtaactaga gcgtaaagct 1921 gagactagac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 1981 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 2041 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 2101 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggtactag 2161 agactagata ctagagctac cgggtagggg aggcgctttt cccaaggcag tctggagcat 2221 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac 2281 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac 2341 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga 2401 cgtgacaaat ggaagtagca cgtctcacaa gtctcgtgca gatggacagc accgctgagc 2461 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct 2521 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg 2581 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt 2641 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctggagc ccactagacc 2701 cgccgccacc atggagacta gaatgaagct actgtcttct atcgaacaag catgcgatat 2761 ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa 2821 gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca 2881 tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc 2941 tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt 3001 aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc 3061 agtggagact gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc 3121 ggaagagagt agtaacaaag gtcaaagaca gttgactgta tcgccggaat ttccggggat 3181 cactagagca tccaacttga acatttcgag aaagcttacc atatcaaccc catcatgctc 3241 tttcgaaaat tcaaatagca catccattcc ttcgcccgct tcctcatctc aaagccacac 3301 tccaatgaga aacatgagct cactctctga taacagcgtt ttcagccgga atatggaaca 3361 atcatcacca atcactccaa gtatgtacca atttggtcag cagcagtcaa acagtatatg 3421 tggtagcacc gttagtgtga atagtctggt gaatacaaat aacaaacaaa ggatctacga 3481 acaaatcacg ggtcctaaca gcaataacgc aaccaatgat tatattgatt tgctaaacct 3541 aaatgagtct aacaaggaaa accaaaatcc cgcaacggcg cattacctca atgggggccc 3601 acccaagaca agcttcatta accatggaat gttcccctcg ccaactggga ccataaatag 3661 cggtaaatct agcagtgcct catctttaat ttcttttggt atgggcaata cccaagtaat 3721 aactagagtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt 3781 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga 3841 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct 3901 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa 3961 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga 4021 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccttgca 4081 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc 4141 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga 4201 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga 4261 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctacccg gcgcctacaa 4321 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta 4381 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agactagagt 4441 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca 4501 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc 4561 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc 4621 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc 4681 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat 4741 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga 4801 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca 4861 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct 4921 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt 4981 caagaccacc tacaaggcca agaagcccgt gcagctaccc ggcgcctaca acgtcaacat 5041 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc 5101 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagactagac ccaagaaaaa 5161 gcgcaaggta actagagcgt aaagctgaga ctagaatagt tgtttaaacg agctcgctag 5221 caaatcaaga gtcctcataa aggaattaaa atcaagagtc ctcataaagt ctagcaaatc 5281 aagagtcctc ataaagctag acaaatcaag agtcctcata aataattcca atcaagagtc 5341 ctcataaagt cgacctgcaa gcatgcaagc tgatccggct gcactagact gtgccttcta 5401 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 5461 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 5521 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 5581 gcaggcatgc tggggatgcg gtgggctcta tgg //