LOCUS BBa_J176154 5597 bp DNA linear 22-Nov-2024 DEFINITION Test miR-luc sensor 13 ACCESSION BBa_J176154 VERSION BBa_J176154.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 1..615 /label=BBa_S04643 misc_feature 616..621 /label=BBa_J176041 RBS 622..639 /label=BBa_J176012 misc_feature 640..645 /label=BBa_J176041 misc_feature 646..1332 /label=BBa_J176022 misc_feature 1333..1338 /label=BBa_J176041 misc_feature 1339..2025 /label=BBa_J176022 misc_feature 2026..2031 /label=BBa_J176041 misc_feature 2032..2052 /label=BBa_J176008 misc_feature 2053..2058 /label=BBa_J176041 terminator 2059..2071 /label=BBa_J176016 misc_feature 646..2071 /label=BBa_S04684 misc_feature 622..2071 /label=BBa_S04738 misc_feature 2072..2077 /label=BBa_J176041 misc_feature 2078..2302 /label=BBa_J176009 misc_feature 622..2302 /label=BBa_S04739 misc_feature 1..2302 /label=BBa_J176096 misc_feature 2303..2308 /label=BBa_J176041 promoter 2309..2676 /label=BBa_J176003 misc_feature 2677..2682 /label=BBa_J176041 RBS 2683..2700 /label=BBa_J176012 misc_feature 2701..2706 /label=BBa_J176041 misc_feature 2707..3165 /label=BBa_J176020 misc_feature 3166..3171 /label=BBa_J176041 misc_feature 3172..3705 /label=BBa_J176018 misc_feature 3706..3711 /label=BBa_J176041 misc_feature 3712..4416 /label=BBa_J176005 misc_feature 4417..4422 /label=BBa_J176041 misc_feature 4423..5127 /label=BBa_J176005 misc_feature 5128..5133 /label=BBa_J176041 misc_feature 5134..5154 /label=BBa_J176008 misc_feature 5155..5160 /label=BBa_J176041 terminator 5161..5173 /label=BBa_J176016 misc_feature 2683..5173 /label=BBa_S04697 misc_feature 2309..5173 /label=BBa_S04741 misc_feature 5174..5179 /label=BBa_J176041 misc_feature 5207..5225 /label=repeat 1 misc_feature 5234..5252 /label=repeat 2 misc_feature 5261..5279 /label=repeat 3 misc_feature 5288..5306 /label=repeat 4 misc_feature 5314..5332 /label=repeat 5 misc_feature 5180..5366 /label=BBa_J176025 misc_feature 5367..5372 /label=BBa_J176041 misc_feature 5373..5597 /label=BBa_J176009 misc_feature 5180..5597 /label=BBa_S04730 misc_feature 2309..5597 /label=BBa_J176101 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acccgccgcc accatggaga ctagaatggc cctgtccaac 661 aagttcatcg gcgacgacat gaagatgacc taccacatgg acggctgcgt gaacggccac 721 tacttcaccg tgaagggcga gggcagcggc aagccctacg agggcaccca gacctccacc 781 ttcaaggtga ccatggccaa cggcggcccc ctggccttct ccttcgacat cctgtccacc 841 gtgttcatgt acggcaaccg ctgcttcacc gcctacccca ccagcatgcc cgactacttc 901 aagcaggcct tccccgacgg catgtcctac gagagaacct tcacctacga ggacggcggc 961 gtggccaccg ccagctggga gatcagcctg aagggcaact gcttcgagca caagtccacc 1021 ttccacggcg tgaacttccc cgccgacggc cccgtgatgg ccaagaagac caccggctgg 1081 gacccctcct tcgagaagat gaccgtgtgc gacggcatct tgaagggcga cgtgaccgcc 1141 ttcctgatgc tgcaaggcgg cggcaactac agatgccagt tccacacctc ctacaagacc 1201 aagaagcccg tgaccatgcc ccccaaccac gtggtggagc accgcatcgc cagaaccgac 1261 ctggacaagg gcggcaacag cgtgcagctg accgagcacg ccgtggccca catcacctcc 1321 gtggtgccct tcactagaat ggccctgtcc aacaagttca tcggcgacga catgaagatg 1381 acctaccaca tggacggctg cgtgaacggc cactacttca ccgtgaaggg cgagggcagc 1441 ggcaagccct acgagggcac ccagacctcc accttcaagg tgaccatggc caacggcggc 1501 cccctggcct tctccttcga catcctgtcc accgtgttca tgtacggcaa ccgctgcttc 1561 accgcctacc ccaccagcat gcccgactac ttcaagcagg ccttccccga cggcatgtcc 1621 tacgagagaa ccttcaccta cgaggacggc ggcgtggcca ccgccagctg ggagatcagc 1681 ctgaagggca actgcttcga gcacaagtcc accttccacg gcgtgaactt ccccgccgac 1741 ggccccgtga tggccaagaa gaccaccggc tgggacccct ccttcgagaa gatgaccgtg 1801 tgcgacggca tcttgaaggg cgacgtgacc gccttcctga tgctgcaagg cggcggcaac 1861 tacagatgcc agttccacac ctcctacaag accaagaagc ccgtgaccat gccccccaac 1921 cacgtggtgg agcaccgcat cgccagaacc gacctggaca agggcggcaa cagcgtgcag 1981 ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc ccttcactag acccaagaaa 2041 aagcgcaagg taactagagc gtaaagctga gactagactg tgccttctag ttgccagcca 2101 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 2161 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 2221 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 2281 ggggatgcgg tgggctctat ggactagaaa actggcctcc gcgccgggct tttggcgcct 2341 cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag 2401 cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc 2461 ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca 2521 ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct 2581 gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg 2641 gcacagctag ttactagacc cgccgccacc atggagacta gacccgccgc caccatggag 2701 actagaatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag 2761 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt 2821 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa 2881 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac 2941 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta 3001 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg 3061 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac 3121 aaaggtcaaa gacagttgac tgtatcgccg gaatttccgg ggatcactag agcatccaac 3181 ttgaacattt cgagaaagct taccatatca accccatcat gctctttcga aaattcaaat 3241 agcacatcca ttccttcgcc cgcttcctca tctcaaagcc acactccaat gagaaacatg 3301 agctcactct ctgataacag cgttttcagc cggaatatgg aacaatcatc accaatcact 3361 ccaagtatgt accaatttgg tcagcagcag tcaaacagta tatgtggtag caccgttagt 3421 gtgaatagtc tggtgaatac aaataacaaa caaaggatct acgaacaaat cacgggtcct 3481 aacagcaata acgcaaccaa tgattatatt gatttgctaa acctaaatga gtctaacaag 3541 gaaaaccaaa atcccgcaac ggcgcattac ctcaatgggg gcccacccaa gacaagcttc 3601 attaaccatg gaatgttccc ctcgccaact gggaccataa atagcggtaa atctagcagt 3661 gcctcatctt taatttcttt tggtatgggc aatacccaag taataactag agtgagcaag 3721 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 3781 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 3841 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 3901 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 3961 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 4021 gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc 4081 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 4141 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 4201 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 4261 acctacaagg ccaagaagcc cgtgcagcta cccggcgcct acaacgtcaa catcaagttg 4321 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 4381 cgccactcca ccggcggcat ggacgagctg tacaagacta gagtgagcaa gggcgaggag 4441 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg 4501 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 4561 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc 4621 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac 4681 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 4741 ggcgtggtga ccgtgaccca ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg 4801 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc 4861 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag 4921 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag 4981 gccaagaagc ccgtgcagct acccggcgcc tacaacgtca acatcaagtt ggacatcacc 5041 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc 5101 accggcggca tggacgagct gtacaagact agacccaaga aaaagcgcaa ggtaactaga 5161 gcgtaaagct gagactagaa tagttgttta aacgagctcg ctagcaaatc aagagtcctc 5221 ataaaggaat taaaatcaag agtcctcata aagtctagca aatcaagagt cctcataaag 5281 ctagacaaat caagagtcct cataaataat tccaatcaag agtcctcata aagtcgacct 5341 gcaagcatgc aagctgatcc ggctgcacta gactgtgcct tctagttgcc agccatctgt 5401 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 5461 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 5521 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 5581 tgcggtgggc tctatgg //