LOCUS BBa_J176155 5745 bp DNA linear 25-Nov-2024 DEFINITION Test miR-luc sensor 14 ACCESSION BBa_J176155 VERSION BBa_J176155.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 1..615 /label=BBa_S04643 misc_feature 616..621 /label=BBa_J176041 RBS 622..639 /label=BBa_J176012 misc_feature 640..645 /label=BBa_J176041 misc_feature 646..1332 /label=BBa_J176022 misc_feature 1333..1338 /label=BBa_J176041 misc_feature 1339..2025 /label=BBa_J176022 misc_feature 2026..2031 /label=BBa_J176041 misc_feature 2032..2052 /label=BBa_J176008 misc_feature 2053..2058 /label=BBa_J176041 terminator 2059..2071 /label=BBa_J176016 misc_feature 646..2071 /label=BBa_S04684 misc_feature 622..2071 /label=BBa_S04738 misc_feature 2072..2077 /label=BBa_J176041 misc_feature 2078..2302 /label=BBa_J176009 misc_feature 622..2302 /label=BBa_S04739 misc_feature 1..2302 /label=BBa_J176096 misc_feature 2303..2308 /label=BBa_J176041 misc_feature 2561..2561 /label=mutated SpeI misc_feature 2819..2819 /label=mutated PstI promoter 2309..2824 /label=BBa_J176028 misc_feature 2825..2830 /label=BBa_J176041 RBS 2831..2848 /label=BBa_J176012 misc_feature 2849..2854 /label=BBa_J176041 misc_feature 2855..3313 /label=BBa_J176020 misc_feature 3314..3319 /label=BBa_J176041 misc_feature 3320..3853 /label=BBa_J176018 misc_feature 3854..3859 /label=BBa_J176041 misc_feature 3860..4564 /label=BBa_J176005 misc_feature 4565..4570 /label=BBa_J176041 misc_feature 4571..5275 /label=BBa_J176005 misc_feature 5276..5281 /label=BBa_J176041 misc_feature 5282..5302 /label=BBa_J176008 misc_feature 5303..5308 /label=BBa_J176041 terminator 5309..5321 /label=BBa_J176016 misc_feature 2309..5321 /label=BBa_S04732 misc_feature 5322..5327 /label=BBa_J176041 misc_feature 5355..5373 /label=repeat 1 misc_feature 5382..5400 /label=repeat 2 misc_feature 5409..5427 /label=repeat 3 misc_feature 5436..5454 /label=repeat 4 misc_feature 5462..5480 /label=repeat 5 misc_feature 5328..5514 /label=BBa_J176025 misc_feature 5515..5520 /label=BBa_J176041 misc_feature 5521..5745 /label=BBa_J176009 misc_feature 5328..5745 /label=BBa_S04730 misc_feature 2309..5745 /label=BBa_S04736 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acccgccgcc accatggaga ctagaatggc cctgtccaac 661 aagttcatcg gcgacgacat gaagatgacc taccacatgg acggctgcgt gaacggccac 721 tacttcaccg tgaagggcga gggcagcggc aagccctacg agggcaccca gacctccacc 781 ttcaaggtga ccatggccaa cggcggcccc ctggccttct ccttcgacat cctgtccacc 841 gtgttcatgt acggcaaccg ctgcttcacc gcctacccca ccagcatgcc cgactacttc 901 aagcaggcct tccccgacgg catgtcctac gagagaacct tcacctacga ggacggcggc 961 gtggccaccg ccagctggga gatcagcctg aagggcaact gcttcgagca caagtccacc 1021 ttccacggcg tgaacttccc cgccgacggc cccgtgatgg ccaagaagac caccggctgg 1081 gacccctcct tcgagaagat gaccgtgtgc gacggcatct tgaagggcga cgtgaccgcc 1141 ttcctgatgc tgcaaggcgg cggcaactac agatgccagt tccacacctc ctacaagacc 1201 aagaagcccg tgaccatgcc ccccaaccac gtggtggagc accgcatcgc cagaaccgac 1261 ctggacaagg gcggcaacag cgtgcagctg accgagcacg ccgtggccca catcacctcc 1321 gtggtgccct tcactagaat ggccctgtcc aacaagttca tcggcgacga catgaagatg 1381 acctaccaca tggacggctg cgtgaacggc cactacttca ccgtgaaggg cgagggcagc 1441 ggcaagccct acgagggcac ccagacctcc accttcaagg tgaccatggc caacggcggc 1501 cccctggcct tctccttcga catcctgtcc accgtgttca tgtacggcaa ccgctgcttc 1561 accgcctacc ccaccagcat gcccgactac ttcaagcagg ccttccccga cggcatgtcc 1621 tacgagagaa ccttcaccta cgaggacggc ggcgtggcca ccgccagctg ggagatcagc 1681 ctgaagggca actgcttcga gcacaagtcc accttccacg gcgtgaactt ccccgccgac 1741 ggccccgtga tggccaagaa gaccaccggc tgggacccct ccttcgagaa gatgaccgtg 1801 tgcgacggca tcttgaaggg cgacgtgacc gccttcctga tgctgcaagg cggcggcaac 1861 tacagatgcc agttccacac ctcctacaag accaagaagc ccgtgaccat gccccccaac 1921 cacgtggtgg agcaccgcat cgccagaacc gacctggaca agggcggcaa cagcgtgcag 1981 ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc ccttcactag acccaagaaa 2041 aagcgcaagg taactagagc gtaaagctga gactagactg tgccttctag ttgccagcca 2101 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 2161 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 2221 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 2281 ggggatgcgg tgggctctat ggactagact accgggtagg ggaggcgctt ttcccaaggc 2341 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct 2401 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc 2461 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg 2521 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac aagtctcgtg cagatggaca 2581 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct 2641 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg 2701 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc 2761 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgga 2821 gcccactaga cccgccgcca ccatggagac tagaatgaag ctactgtctt ctatcgaaca 2881 agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc 2941 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct 3001 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct 3061 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat 3121 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga 3181 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc 3241 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgccgga 3301 atttccgggg atcactagag catccaactt gaacatttcg agaaagctta ccatatcaac 3361 cccatcatgc tctttcgaaa attcaaatag cacatccatt ccttcgcccg cttcctcatc 3421 tcaaagccac actccaatga gaaacatgag ctcactctct gataacagcg ttttcagccg 3481 gaatatggaa caatcatcac caatcactcc aagtatgtac caatttggtc agcagcagtc 3541 aaacagtata tgtggtagca ccgttagtgt gaatagtctg gtgaatacaa ataacaaaca 3601 aaggatctac gaacaaatca cgggtcctaa cagcaataac gcaaccaatg attatattga 3661 tttgctaaac ctaaatgagt ctaacaagga aaaccaaaat cccgcaacgg cgcattacct 3721 caatgggggc ccacccaaga caagcttcat taaccatgga atgttcccct cgccaactgg 3781 gaccataaat agcggtaaat ctagcagtgc ctcatcttta atttcttttg gtatgggcaa 3841 tacccaagta ataactagag tgagcaaggg cgaggaggat aacatggcca tcatcaagga 3901 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga 3961 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 4021 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa 4081 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg 4141 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 4201 ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 4261 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat 4321 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg 4381 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctacc 4441 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 4501 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta 4561 caagactaga gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg 4621 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg 4681 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc 4741 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt 4801 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg 4861 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctcctt 4921 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg 4981 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga 5041 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta 5101 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctac ccggcgccta 5161 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca 5221 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagactag 5281 acccaagaaa aagcgcaagg taactagagc gtaaagctga gactagaata gttgtttaaa 5341 cgagctcgct agcaaatcaa gagtcctcat aaaggaatta aaatcaagag tcctcataaa 5401 gtctagcaaa tcaagagtcc tcataaagct agacaaatca agagtcctca taaataattc 5461 caatcaagag tcctcataaa gtcgacctgc aagcatgcaa gctgatccgg ctgcactaga 5521 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 5581 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 5641 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 5701 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatgg //