LOCUS BBa_J176156 5817 bp DNA linear 22-Nov-2024 DEFINITION Test miR-luc sensor 15 ACCESSION BBa_J176156 VERSION BBa_J176156.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 1..615 /label=BBa_S04643 misc_feature 616..621 /label=BBa_J176041 RBS 622..639 /label=BBa_J176012 misc_feature 640..645 /label=BBa_J176041 misc_feature 646..1332 /label=BBa_J176022 misc_feature 1333..1338 /label=BBa_J176041 misc_feature 1339..2025 /label=BBa_J176022 misc_feature 2026..2031 /label=BBa_J176041 misc_feature 2032..2052 /label=BBa_J176008 misc_feature 2053..2058 /label=BBa_J176041 terminator 2059..2071 /label=BBa_J176016 misc_feature 646..2071 /label=BBa_S04684 misc_feature 622..2071 /label=BBa_S04738 misc_feature 2072..2077 /label=BBa_J176041 misc_feature 2078..2302 /label=BBa_J176009 misc_feature 622..2302 /label=BBa_S04739 misc_feature 1..2302 /label=BBa_J176096 misc_feature 2303..2308 /label=BBa_J176041 promoter 2309..2896 /label=BBa_J176027 misc_feature 2897..2902 /label=BBa_J176041 RBS 2903..2920 /label=BBa_J176012 misc_feature 2921..2926 /label=BBa_J176041 misc_feature 2927..3385 /label=BBa_J176020 misc_feature 3386..3391 /label=BBa_J176041 misc_feature 3392..3925 /label=BBa_J176018 misc_feature 3926..3931 /label=BBa_J176041 misc_feature 3932..4636 /label=BBa_J176005 misc_feature 4637..4642 /label=BBa_J176041 misc_feature 4643..5347 /label=BBa_J176005 misc_feature 5348..5353 /label=BBa_J176041 misc_feature 5354..5374 /label=BBa_J176008 misc_feature 5375..5380 /label=BBa_J176041 terminator 5381..5393 /label=BBa_J176016 misc_feature 2903..5393 /label=BBa_S04697 misc_feature 2309..5393 /label=BBa_S04742 misc_feature 5394..5399 /label=BBa_J176041 misc_feature 5427..5445 /label=repeat 1 misc_feature 5454..5472 /label=repeat 2 misc_feature 5481..5499 /label=repeat 3 misc_feature 5508..5526 /label=repeat 4 misc_feature 5534..5552 /label=repeat 5 misc_feature 5400..5586 /label=BBa_J176025 misc_feature 5587..5592 /label=BBa_J176041 misc_feature 5593..5817 /label=BBa_J176009 misc_feature 5400..5817 /label=BBa_S04730 misc_feature 2309..5817 /label=BBa_J176103 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acccgccgcc accatggaga ctagaatggc cctgtccaac 661 aagttcatcg gcgacgacat gaagatgacc taccacatgg acggctgcgt gaacggccac 721 tacttcaccg tgaagggcga gggcagcggc aagccctacg agggcaccca gacctccacc 781 ttcaaggtga ccatggccaa cggcggcccc ctggccttct ccttcgacat cctgtccacc 841 gtgttcatgt acggcaaccg ctgcttcacc gcctacccca ccagcatgcc cgactacttc 901 aagcaggcct tccccgacgg catgtcctac gagagaacct tcacctacga ggacggcggc 961 gtggccaccg ccagctggga gatcagcctg aagggcaact gcttcgagca caagtccacc 1021 ttccacggcg tgaacttccc cgccgacggc cccgtgatgg ccaagaagac caccggctgg 1081 gacccctcct tcgagaagat gaccgtgtgc gacggcatct tgaagggcga cgtgaccgcc 1141 ttcctgatgc tgcaaggcgg cggcaactac agatgccagt tccacacctc ctacaagacc 1201 aagaagcccg tgaccatgcc ccccaaccac gtggtggagc accgcatcgc cagaaccgac 1261 ctggacaagg gcggcaacag cgtgcagctg accgagcacg ccgtggccca catcacctcc 1321 gtggtgccct tcactagaat ggccctgtcc aacaagttca tcggcgacga catgaagatg 1381 acctaccaca tggacggctg cgtgaacggc cactacttca ccgtgaaggg cgagggcagc 1441 ggcaagccct acgagggcac ccagacctcc accttcaagg tgaccatggc caacggcggc 1501 cccctggcct tctccttcga catcctgtcc accgtgttca tgtacggcaa ccgctgcttc 1561 accgcctacc ccaccagcat gcccgactac ttcaagcagg ccttccccga cggcatgtcc 1621 tacgagagaa ccttcaccta cgaggacggc ggcgtggcca ccgccagctg ggagatcagc 1681 ctgaagggca actgcttcga gcacaagtcc accttccacg gcgtgaactt ccccgccgac 1741 ggccccgtga tggccaagaa gaccaccggc tgggacccct ccttcgagaa gatgaccgtg 1801 tgcgacggca tcttgaaggg cgacgtgacc gccttcctga tgctgcaagg cggcggcaac 1861 tacagatgcc agttccacac ctcctacaag accaagaagc ccgtgaccat gccccccaac 1921 cacgtggtgg agcaccgcat cgccagaacc gacctggaca agggcggcaa cagcgtgcag 1981 ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc ccttcactag acccaagaaa 2041 aagcgcaagg taactagagc gtaaagctga gactagactg tgccttctag ttgccagcca 2101 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 2161 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 2221 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 2281 ggggatgcgg tgggctctat ggactagagt tgacattgat tattggctag ttattaatag 2341 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 2401 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 2461 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 2521 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 2581 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 2641 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg 2701 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc 2761 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa 2821 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc 2881 tatataagca gagctcacta gacccgccgc caccatggag actagaatga agctactgtc 2941 ttctatcgaa caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa 3001 accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa 3061 aaggtctccg ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga 3121 acagctattt ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc 3181 tttacaggat ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga 3241 tgccgtcaca gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca 3301 tagaataagt gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac 3361 tgtatcgccg gaatttccgg ggatcactag agcatccaac ttgaacattt cgagaaagct 3421 taccatatca accccatcat gctctttcga aaattcaaat agcacatcca ttccttcgcc 3481 cgcttcctca tctcaaagcc acactccaat gagaaacatg agctcactct ctgataacag 3541 cgttttcagc cggaatatgg aacaatcatc accaatcact ccaagtatgt accaatttgg 3601 tcagcagcag tcaaacagta tatgtggtag caccgttagt gtgaatagtc tggtgaatac 3661 aaataacaaa caaaggatct acgaacaaat cacgggtcct aacagcaata acgcaaccaa 3721 tgattatatt gatttgctaa acctaaatga gtctaacaag gaaaaccaaa atcccgcaac 3781 ggcgcattac ctcaatgggg gcccacccaa gacaagcttc attaaccatg gaatgttccc 3841 ctcgccaact gggaccataa atagcggtaa atctagcagt gcctcatctt taatttcttt 3901 tggtatgggc aatacccaag taataactag agtgagcaag ggcgaggagg ataacatggc 3961 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 4021 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 4081 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 4141 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 4201 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 4261 cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 4321 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 4381 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 4441 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 4501 cgtgcagcta cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 4561 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 4621 ggacgagctg tacaagacta gagtgagcaa gggcgaggag gataacatgg ccatcatcaa 4681 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 4741 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 4801 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 4861 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 4921 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 4981 ggactcctcc ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 5041 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 5101 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 5161 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 5221 acccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 5281 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 5341 gtacaagact agacccaaga aaaagcgcaa ggtaactaga gcgtaaagct gagactagaa 5401 tagttgttta aacgagctcg ctagcaaatc aagagtcctc ataaaggaat taaaatcaag 5461 agtcctcata aagtctagca aatcaagagt cctcataaag ctagacaaat caagagtcct 5521 cataaataat tccaatcaag agtcctcata aagtcgacct gcaagcatgc aagctgatcc 5581 ggctgcacta gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 5641 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 5701 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 5761 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgg //