LOCUS BBa_J176158 5844 bp DNA linear 26-Dec-2024 DEFINITION Test miR-luc sensor 17 ACCESSION BBa_J176158 VERSION BBa_J176158.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 616..621 /label=BBa_J176041 misc_feature 622..638 /label=Gal4 misc_feature 641..657 /label=Gal4 misc_feature 660..676 /label=Gal4 misc_feature 679..695 /label=Gal4 misc_feature 698..714 /label=Gal4 misc_feature 622..714 /label=BBa_J176019 misc_feature 1..714 /label=BBa_S04655 misc_feature 715..720 /label=BBa_J176041 RBS 721..738 /label=BBa_J176012 misc_feature 739..744 /label=BBa_J176041 misc_feature 745..1431 /label=BBa_J176022 misc_feature 1432..1437 /label=BBa_J176041 misc_feature 1438..2124 /label=BBa_J176022 misc_feature 2125..2130 /label=BBa_J176041 misc_feature 2131..2151 /label=BBa_J176008 misc_feature 2152..2157 /label=BBa_J176041 terminator 2158..2170 /label=BBa_J176016 misc_feature 745..2170 /label=BBa_S04684 misc_feature 721..2170 /label=BBa_S04738 misc_feature 2171..2176 /label=BBa_J176041 misc_feature 2177..2401 /label=BBa_J176009 misc_feature 721..2401 /label=BBa_S04739 misc_feature 1..2401 /label=BBa_J176091 misc_feature 2402..2407 /label=BBa_J176041 misc_feature 2660..2660 /label=mutated SpeI misc_feature 2918..2918 /label=mutated PstI promoter 2408..2923 /label=BBa_J176028 misc_feature 2924..2929 /label=BBa_J176041 RBS 2930..2947 /label=BBa_J176012 misc_feature 2948..2953 /label=BBa_J176041 misc_feature 2954..3412 /label=BBa_J176020 misc_feature 3413..3418 /label=BBa_J176041 misc_feature 3419..3952 /label=BBa_J176018 misc_feature 3953..3958 /label=BBa_J176041 misc_feature 3959..4663 /label=BBa_J176005 misc_feature 4664..4669 /label=BBa_J176041 misc_feature 4670..5374 /label=BBa_J176005 misc_feature 5375..5380 /label=BBa_J176041 misc_feature 5381..5401 /label=BBa_J176008 misc_feature 5402..5407 /label=BBa_J176041 terminator 5408..5420 /label=BBa_J176016 misc_feature 2408..5420 /label=BBa_S04732 misc_feature 5421..5426 /label=BBa_J176041 misc_feature 5454..5472 /label=repeat 1 misc_feature 5481..5499 /label=repeat 2 misc_feature 5508..5526 /label=repeat 3 misc_feature 5535..5553 /label=repeat 4 misc_feature 5561..5579 /label=repeat 5 misc_feature 5427..5613 /label=BBa_J176025 misc_feature 5614..5619 /label=BBa_J176041 misc_feature 5620..5844 /label=BBa_J176009 misc_feature 5427..5844 /label=BBa_S04730 misc_feature 2408..5844 /label=BBa_S04736 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acggagtact gtcctccgag cggagtactg tcctccgagc 661 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgactaga 721 cccgccgcca ccatggagac tagaatggcc ctgtccaaca agttcatcgg cgacgacatg 781 aagatgacct accacatgga cggctgcgtg aacggccact acttcaccgt gaagggcgag 841 ggcagcggca agccctacga gggcacccag acctccacct tcaaggtgac catggccaac 901 ggcggccccc tggccttctc cttcgacatc ctgtccaccg tgttcatgta cggcaaccgc 961 tgcttcaccg cctaccccac cagcatgccc gactacttca agcaggcctt ccccgacggc 1021 atgtcctacg agagaacctt cacctacgag gacggcggcg tggccaccgc cagctgggag 1081 atcagcctga agggcaactg cttcgagcac aagtccacct tccacggcgt gaacttcccc 1141 gccgacggcc ccgtgatggc caagaagacc accggctggg acccctcctt cgagaagatg 1201 accgtgtgcg acggcatctt gaagggcgac gtgaccgcct tcctgatgct gcaaggcggc 1261 ggcaactaca gatgccagtt ccacacctcc tacaagacca agaagcccgt gaccatgccc 1321 cccaaccacg tggtggagca ccgcatcgcc agaaccgacc tggacaaggg cggcaacagc 1381 gtgcagctga ccgagcacgc cgtggcccac atcacctccg tggtgccctt cactagaatg 1441 gccctgtcca acaagttcat cggcgacgac atgaagatga cctaccacat ggacggctgc 1501 gtgaacggcc actacttcac cgtgaagggc gagggcagcg gcaagcccta cgagggcacc 1561 cagacctcca ccttcaaggt gaccatggcc aacggcggcc ccctggcctt ctccttcgac 1621 atcctgtcca ccgtgttcat gtacggcaac cgctgcttca ccgcctaccc caccagcatg 1681 cccgactact tcaagcaggc cttccccgac ggcatgtcct acgagagaac cttcacctac 1741 gaggacggcg gcgtggccac cgccagctgg gagatcagcc tgaagggcaa ctgcttcgag 1801 cacaagtcca ccttccacgg cgtgaacttc cccgccgacg gccccgtgat ggccaagaag 1861 accaccggct gggacccctc cttcgagaag atgaccgtgt gcgacggcat cttgaagggc 1921 gacgtgaccg ccttcctgat gctgcaaggc ggcggcaact acagatgcca gttccacacc 1981 tcctacaaga ccaagaagcc cgtgaccatg ccccccaacc acgtggtgga gcaccgcatc 2041 gccagaaccg acctggacaa gggcggcaac agcgtgcagc tgaccgagca cgccgtggcc 2101 cacatcacct ccgtggtgcc cttcactaga cccaagaaaa agcgcaaggt aactagagcg 2161 taaagctgag actagactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 2221 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 2281 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 2341 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 2401 gactagacta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 2461 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 2521 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 2581 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 2641 tggaagtagc acgtctcaca agtctcgtgc agatggacag caccgctgag caatggaagc 2701 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga 2761 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg 2821 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc 2881 tgttctcctc ttcctcatct ccgggccttt cgacctggag cccactagac ccgccgccac 2941 catggagact agaatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact 3001 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg 3061 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga 3121 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga 3181 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt 3241 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac 3301 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag 3361 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa tttccgggga tcactagagc 3421 atccaacttg aacatttcga gaaagcttac catatcaacc ccatcatgct ctttcgaaaa 3481 ttcaaatagc acatccattc cttcgcccgc ttcctcatct caaagccaca ctccaatgag 3541 aaacatgagc tcactctctg ataacagcgt tttcagccgg aatatggaac aatcatcacc 3601 aatcactcca agtatgtacc aatttggtca gcagcagtca aacagtatat gtggtagcac 3661 cgttagtgtg aatagtctgg tgaatacaaa taacaaacaa aggatctacg aacaaatcac 3721 gggtcctaac agcaataacg caaccaatga ttatattgat ttgctaaacc taaatgagtc 3781 taacaaggaa aaccaaaatc ccgcaacggc gcattacctc aatgggggcc cacccaagac 3841 aagcttcatt aaccatggaa tgttcccctc gccaactggg accataaata gcggtaaatc 3901 tagcagtgcc tcatctttaa tttcttttgg tatgggcaat acccaagtaa taactagagt 3961 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca 4021 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc 4081 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc 4141 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc 4201 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat 4261 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc aggacggcga 4321 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca 4381 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct 4441 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt 4501 caagaccacc tacaaggcca agaagcccgt gcagctaccc ggcgcctaca acgtcaacat 4561 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc 4621 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagactagag tgagcaaggg 4681 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 4741 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 4801 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 4861 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 4921 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 4981 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta 5041 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 5101 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 5161 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 5221 ctacaaggcc aagaagcccg tgcagctacc cggcgcctac aacgtcaaca tcaagttgga 5281 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 5341 ccactccacc ggcggcatgg acgagctgta caagactaga cccaagaaaa agcgcaaggt 5401 aactagagcg taaagctgag actagaatag ttgtttaaac gagctcgcta gcaaatcaag 5461 agtcctcata aaggaattaa aatcaagagt cctcataaag tctagcaaat caagagtcct 5521 cataaagcta gacaaatcaa gagtcctcat aaataattcc aatcaagagt cctcataaag 5581 tcgacctgca agcatgcaag ctgatccggc tgcactagac tgtgccttct agttgccagc 5641 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 5701 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 5761 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 5821 ctggggatgc ggtgggctct atgg //