##gff-version 3
##sequence-region BBa_J176158 1 5844
BBa_J176158	.	engineered_region	1	2401	.	+	0	ID=BBa_J176091;Name=BBa_J176091
BBa_J176158	.	engineered_region	721	2401	.	+	0	ID=BBa_S04739;Name=BBa_S04739;Parent=BBa_J176091
BBa_J176158	.	sequence_feature	2171	2176	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04739
BBa_J176158	.	engineered_region	721	2170	.	+	0	ID=BBa_S04738;Name=BBa_S04738;Parent=BBa_S04739
BBa_J176158	.	ribosome_entry_site	721	738	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04738
BBa_J176158	.	sequence_feature	739	744	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04738
BBa_J176158	.	engineered_region	745	2170	.	+	0	ID=BBa_S04684;Name=BBa_S04684;Parent=BBa_S04738
BBa_J176158	.	sequence_feature	2152	2157	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176158	.	tag	2131	2151	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04684
BBa_J176158	.	polypeptide_domain	745	1431	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176158	.	sequence_feature	1432	1437	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176158	.	polypeptide_domain	1438	2124	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04684
BBa_J176158	.	sequence_feature	2125	2130	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04684
BBa_J176158	.	terminator	2158	2170	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04684
BBa_J176158	.	tag	2177	2401	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04739
BBa_J176158	.	sequence_feature	715	720	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176091
BBa_J176158	.	engineered_region	1	714	.	+	0	ID=BBa_S04655;Name=BBa_S04655;Parent=BBa_J176091
BBa_J176158	.	sequence_feature	622	714	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176158	.	non_covalent_binding_site	641	657	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	non_covalent_binding_site	660	676	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	non_covalent_binding_site	698	714	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	non_covalent_binding_site	622	638	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	non_covalent_binding_site	679	695	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	sequence_feature	517	522	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176158	.	promoter	1	516	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04655
BBa_J176158	.	sequence_alteration	511	511	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176158	.	sequence_alteration	253	253	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176158	.	sequence_feature	616	621	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176158	.	sequence_feature	523	615	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176158	.	non_covalent_binding_site	542	558	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	non_covalent_binding_site	561	577	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	non_covalent_binding_site	599	615	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	non_covalent_binding_site	523	539	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	non_covalent_binding_site	580	596	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176158	.	sequence_feature	2402	2407	.	+	0	ID=BBa_J176041;Name=BBa_J176041
BBa_J176158	.	engineered_region	2408	5844	.	+	0	ID=BBa_S04736;Name=BBa_S04736
BBa_J176158	.	sequence_feature	5421	5426	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04736
BBa_J176158	.	engineered_region	5427	5844	.	+	0	ID=BBa_S04730;Name=BBa_S04730;Parent=BBa_S04736
BBa_J176158	.	sequence_feature	5614	5619	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04730
BBa_J176158	.	mature_transcript_region	5427	5613	.	+	0	ID=BBa_J176025;Name=BBa_J176025;Parent=BBa_S04730
BBa_J176158	.	sequence_feature	5481	5499	.	+	0	ID=annotation2166808;Name=repeat 2;Parent=BBa_J176025
BBa_J176158	.	sequence_feature	5508	5526	.	+	0	ID=annotation2166809;Name=repeat 3;Parent=BBa_J176025
BBa_J176158	.	sequence_feature	5561	5579	.	+	0	ID=annotation2166811;Name=repeat 5;Parent=BBa_J176025
BBa_J176158	.	sequence_feature	5535	5553	.	+	0	ID=annotation2166810;Name=repeat 4;Parent=BBa_J176025
BBa_J176158	.	sequence_feature	5454	5472	.	+	0	ID=annotation2166807;Name=repeat 1;Parent=BBa_J176025
BBa_J176158	.	tag	5620	5844	.	+	0	ID=BBa_J176009;Name=BBa_J176009;Parent=BBa_S04730
BBa_J176158	.	engineered_region	2408	5420	.	+	0	ID=BBa_S04732;Name=BBa_S04732;Parent=BBa_S04736
BBa_J176158	.	ribosome_entry_site	2930	2947	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04732
BBa_J176158	.	terminator	5408	5420	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04732
BBa_J176158	.	sequence_feature	5402	5407	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176158	.	polypeptide_domain	4670	5374	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_S04732
BBa_J176158	.	polypeptide_domain	3959	4663	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_S04732
BBa_J176158	.	sequence_feature	4664	4669	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176158	.	tag	3419	3952	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_S04732
BBa_J176158	.	tag	5381	5401	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04732
BBa_J176158	.	polypeptide_domain	2954	3412	.	+	0	ID=BBa_J176020;Name=BBa_J176020;Parent=BBa_S04732
BBa_J176158	.	sequence_feature	3413	3418	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176158	.	sequence_feature	2948	2953	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176158	.	sequence_feature	5375	5380	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176158	.	sequence_feature	2924	2929	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
BBa_J176158	.	promoter	2408	2923	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04732
BBa_J176158	.	sequence_alteration	2918	2918	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176158	.	sequence_alteration	2660	2660	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176158	.	sequence_feature	3953	3958	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04732
>BBa_J176158
ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctac
acaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcg
cgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatgga
agtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggc
caatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggc
tcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct
gttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtc
ctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacggagtactgt
cctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcct
ccgactagacccgccgccaccatggagactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacct
accacatggacggctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcaccca
gacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatg
tacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcct
acgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcga
gcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccc
tccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggca
actacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccg
catcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtg
gtgcccttcactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcg
tgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggt
gaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttc
accgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacct
acgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttcca
cggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgacc
gtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttcc
acacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacct
ggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagaccc
aagaaaaagcgcaaggtaactagagcgtaaagctgagactagactgtgccttctagttgccagccatctgttgtttgcc
cctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgca
ttgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagc
aggcatgctggggatgcggtgggctctatggactagactaccgggtaggggaggcgcttttcccaaggcagtctggagc
atgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggt
aggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccg
ccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgc
tgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctg
ggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccgg
cattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagccc
actagacccgccgccaccatggagactagaatgaagctactgtcttctatcgaacaagcatgcgatatttgccgactta
aaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaa
aaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttcta
ctgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggat
tatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacatt
gagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccg
gaatttccggggatcactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcg
aaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcact
ctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcag
cagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaac
aaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaa
ccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcg
ccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaa
taactagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggaggg
ctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctg
aaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacg
tgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaactt
cgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgc
ggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtacc
ccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaa
gaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccac
aacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtaca
agactagagtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggaggg
ctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctg
aaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacg
tgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaactt
cgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgc
ggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtacc
ccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaa
gaccacctacaaggccaagaagcccgtgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccac
aacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtaca
agactagacccaagaaaaagcgcaaggtaactagagcgtaaagctgagactagaatagttgtttaaacgagctcgctag
caaatcaagagtcctcataaaggaattaaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagcta
gacaaatcaagagtcctcataaataattccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccgg
ctgcactagactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtg
ccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctgggggg
tggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatgg