##gff-version 3
##sequence-region BBa_J176169 1 8254
BBa_J176169	.	engineered_region	721	3184	.	+	0	ID=BBa_S04704;Name=BBa_S04704
BBa_J176169	.	terminator	2698	2710	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04704
BBa_J176169	.	sequence_feature	1279	1284	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176169	.	sequence_feature	739	744	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176169	.	polypeptide_domain	1285	1971	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04704
BBa_J176169	.	sequence_feature	1972	1977	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176169	.	sequence_feature	2665	2670	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176169	.	tag	2671	2691	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04704
BBa_J176169	.	sequence_feature	2711	2716	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176169	.	sequence_feature	2692	2697	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04704
BBa_J176169	.	tag	2717	3184	.	+	0	ID=BBa_J176042;Name=BBa_J176042;Parent=BBa_S04704
BBa_J176169	.	sequence_feature	2844	2844	.	+	0	ID=annotation2156673;Name=questionable base call;Parent=BBa_J176042
BBa_J176169	.	polypeptide_domain	1978	2664	.	+	0	ID=BBa_J176022;Name=BBa_J176022;Parent=BBa_S04704
BBa_J176169	.	tag	745	1278	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_S04704
BBa_J176169	.	ribosome_entry_site	721	738	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04704
BBa_J176169	.	sequence_feature	3185	3190	.	+	0	ID=BBa_J176041;Name=BBa_J176041
BBa_J176169	.	engineered_region	1	714	.	+	0	ID=BBa_S04655;Name=BBa_S04655
BBa_J176169	.	sequence_feature	523	615	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176169	.	non_covalent_binding_site	542	558	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	non_covalent_binding_site	523	539	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	non_covalent_binding_site	599	615	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	non_covalent_binding_site	580	596	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	non_covalent_binding_site	561	577	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	promoter	1	516	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04655
BBa_J176169	.	sequence_alteration	511	511	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176169	.	sequence_alteration	253	253	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176169	.	sequence_feature	622	714	.	+	0	ID=BBa_J176019;Name=BBa_J176019;Parent=BBa_S04655
BBa_J176169	.	non_covalent_binding_site	641	657	.	+	0	ID=annotation2159560;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	non_covalent_binding_site	622	638	.	+	0	ID=annotation2159559;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	non_covalent_binding_site	698	714	.	+	0	ID=annotation2159563;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	non_covalent_binding_site	679	695	.	+	0	ID=annotation2159562;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	non_covalent_binding_site	660	676	.	+	0	ID=annotation2159561;Name=Gal4;Parent=BBa_J176019
BBa_J176169	.	sequence_feature	517	522	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176169	.	sequence_feature	616	621	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04655
BBa_J176169	.	sequence_feature	3191	8254	.	+	0	ID=BBa_J176047;Name=BBa_J176047
BBa_J176169	.	engineered_region	6902	8254	.	+	0	ID=BBa_S04691;Name=BBa_S04691;Parent=BBa_J176047
BBa_J176169	.	mature_transcript_region	6902	7088	.	+	0	ID=BBa_J176025;Name=BBa_J176025;Parent=BBa_S04691
BBa_J176169	.	sequence_feature	6956	6974	.	+	0	ID=annotation2166808;Name=repeat 2;Parent=BBa_J176025
BBa_J176169	.	sequence_feature	6929	6947	.	+	0	ID=annotation2166807;Name=repeat 1;Parent=BBa_J176025
BBa_J176169	.	sequence_feature	7036	7054	.	+	0	ID=annotation2166811;Name=repeat 5;Parent=BBa_J176025
BBa_J176169	.	sequence_feature	7010	7028	.	+	0	ID=annotation2166810;Name=repeat 4;Parent=BBa_J176025
BBa_J176169	.	sequence_feature	6983	7001	.	+	0	ID=annotation2166809;Name=repeat 3;Parent=BBa_J176025
BBa_J176169	.	sequence_feature	7089	7094	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04691
BBa_J176169	.	sequence_feature	7909	7914	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04691
BBa_J176169	.	tag	7095	7562	.	+	0	ID=BBa_J176042;Name=BBa_J176042;Parent=BBa_S04691
BBa_J176169	.	sequence_feature	7222	7222	.	+	0	ID=annotation2156673;Name=questionable base call;Parent=BBa_J176042
BBa_J176169	.	sequence_feature	7915	8254	.	+	0	ID=BBa_J176021;Name=BBa_J176021;Parent=BBa_S04691
BBa_J176169	.	sequence_feature	7569	7908	.	+	0	ID=BBa_J176021;Name=BBa_J176021;Parent=BBa_S04691
BBa_J176169	.	sequence_feature	7563	7568	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04691
BBa_J176169	.	engineered_region	3191	6895	.	+	0	ID=BBa_S04700;Name=BBa_S04700;Parent=BBa_J176047
BBa_J176169	.	sequence_feature	6139	6144	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	promoter	3883	4398	.	+	0	ID=BBa_J176028;Name=BBa_J176028;Parent=BBa_S04700
BBa_J176169	.	sequence_alteration	4393	4393	.	+	0	ID=annotation2166554;Name=mutated PstI;Parent=BBa_J176028
BBa_J176169	.	sequence_alteration	4135	4135	.	+	0	ID=annotation2166553;Name=mutated SpeI;Parent=BBa_J176028
BBa_J176169	.	sequence_feature	6877	6882	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	tag	4894	5427	.	+	0	ID=BBa_J176018;Name=BBa_J176018;Parent=BBa_S04700
BBa_J176169	.	polypeptide_domain	5434	6138	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	4399	4404	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	terminator	6883	6895	.	+	0	ID=BBa_J176016;Name=BBa_J176016;Parent=BBa_S04700
BBa_J176169	.	polypeptide_domain	4429	4887	.	+	0	ID=BBa_J176020;Name=BBa_J176020;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	3537	3876	.	+	0	ID=BBa_J176021;Name=BBa_J176021;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	4423	4428	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	3191	3530	.	+	0	ID=BBa_J176021;Name=BBa_J176021;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	3531	3536	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	ribosome_entry_site	4405	4422	.	+	0	ID=BBa_J176012;Name=BBa_J176012;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	3877	3882	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	tag	6856	6876	.	+	0	ID=BBa_J176008;Name=BBa_J176008;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	5428	5433	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	polypeptide_domain	6145	6849	.	+	0	ID=BBa_J176005;Name=BBa_J176005;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	4888	4893	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	6850	6855	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_S04700
BBa_J176169	.	sequence_feature	6896	6901	.	+	0	ID=BBa_J176041;Name=BBa_J176041;Parent=BBa_J176047
BBa_J176169	.	sequence_feature	715	720	.	+	0	ID=BBa_J176041;Name=BBa_J176041
>BBa_J176169
ctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctac
acaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcg
cgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatgga
agtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggc
caatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggc
tcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgct
gttctcctcttcctcatctccgggcctttcgacctggagcccactagacggagtactgtcctccgagcggagtactgtc
ctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgactagacggagtactgt
cctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcct
ccgactagacccgccgccaccatggagactagagcatccaacttgaacatttcgagaaagcttaccatatcaaccccat
catgctctttcgaaaattcaaatagcacatccattccttcgcccgcttcctcatctcaaagccacactccaatgagaaa
catgagctcactctctgataacagcgttttcagccggaatatggaacaatcatcaccaatcactccaagtatgtaccaa
tttggtcagcagcagtcaaacagtatatgtggtagcaccgttagtgtgaatagtctggtgaatacaaataacaaacaaa
ggatctacgaacaaatcacgggtcctaacagcaataacgcaaccaatgattatattgatttgctaaacctaaatgagtc
taacaaggaaaaccaaaatcccgcaacggcgcattacctcaatgggggcccacccaagacaagcttcattaaccatgga
atgttcccctcgccaactgggaccataaatagcggtaaatctagcagtgcctcatctttaatttcttttggtatgggca
atacccaagtaataactagaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacgg
ctgcgtgaacggccactacttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttc
aaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgct
gcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaacctt
cacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccacc
ttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaaga
tgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgcca
gttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaacc
gacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcacta
gaatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccacta
cttcaccgtgaagggcgagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaac
ggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctacccca
ccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcgg
cgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttc
cccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggca
tcttgaagggcgacgtgaccgccttcctgatgctgcaaggcggcggcaactacagatgccagttccacacctcctacaa
gaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggc
aacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttcactagacccaagaaaaagcgca
aggtaactagagcgtaaagctgagactagaagacatgataagatacattgatgagtttggacaaaccacaactagaatg
cagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaag
ttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacct
ctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtcctggccggaccacgctatctgtgcaaggtcccc
ggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcgaagactcgggcggcgccctgcccgtcccaccag
gtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgaccttcagcatcgccggcatgtccccctggcggacgg
gaagtatccagctcgaccaagcttactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgat
caggggagctcacggggacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcag
cgaccgcccggggctccgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacg
gggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacgggg
aaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggcgcgccactagattaattaactcgagtccta
aagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatgtaa
ttacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatcccgag
ccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgctctt
tgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcgaggc
gcgccactagactaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggca
cttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttgg
tggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacg
tgacaaatggaagtagcacgtctcacaagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggccttt
ggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggct
caggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacg
tctgccgcgctgttctcctcttcctcatctccgggcctttcgacctggagcccactagacccgccgccaccatggagac
tagaatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaa
ccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactaggg
cacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttga
catgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaa
gatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacat
catcatcggaagagagtagtaacaaaggtcaaagacagttgactgtatcgccggaatttccggggatcactagagcatc
caacttgaacatttcgagaaagcttaccatatcaaccccatcatgctctttcgaaaattcaaatagcacatccattcct
tcgcccgcttcctcatctcaaagccacactccaatgagaaacatgagctcactctctgataacagcgttttcagccgga
atatggaacaatcatcaccaatcactccaagtatgtaccaatttggtcagcagcagtcaaacagtatatgtggtagcac
cgttagtgtgaatagtctggtgaatacaaataacaaacaaaggatctacgaacaaatcacgggtcctaacagcaataac
gcaaccaatgattatattgatttgctaaacctaaatgagtctaacaaggaaaaccaaaatcccgcaacggcgcattacc
tcaatgggggcccacccaagacaagcttcattaaccatggaatgttcccctcgccaactgggaccataaatagcggtaa
atctagcagtgcctcatctttaatttcttttggtatgggcaatacccaagtaataactagagtgagcaagggcgaggag
gataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgaga
tcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcc
cttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgac
tacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtga
cccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccc
cgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgag
atcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccg
tgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaaca
gtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagactagagtgagcaagggcgaggag
gataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgaga
tcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcc
cttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgac
tacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtga
cccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccc
cgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgag
atcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccg
tgcagctacccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaaca
gtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagactagacccaagaaaaagcgcaag
gtaactagagcgtaaagctgagactagaatagttgtttaaacgagctcgctagcaaatcaagagtcctcataaaggaat
taaaatcaagagtcctcataaagtctagcaaatcaagagtcctcataaagctagacaaatcaagagtcctcataaataa
ttccaatcaagagtcctcataaagtcgacctgcaagcatgcaagctgatccggctgcactagaagacatgataagatac
attgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctt
tatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggggga
ggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcctgcaagcctcgtcgtc
ctggccggaccacgctatctgtgcaaggtccccggccccggacgcgcgctccatgagcagagcgcccgccgccgaggcg
aagactcgggcggcgccctgcccgtcccaccaggtcaacaggcggtaaccggcctcttcatcgggaatgcgcgcgacct
tcagcatcgccggcatgtccccctggcggacgggaagtatccagctcgaccaagcttactagattaattaactcgagtc
ctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggggacagcccccccccaaagcccccagggatg
taattacgtccctcccccgctagggggcagcagcgaccgcccggggctccgctccggtccggcgctccccccgcatccc
gagccggcagcgtgcggggacagcccgggcacggggaaggtggcacgggatcgctttcctctgaacgcttctcgctgct
ctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatcttctacactgggacgcaaggattatcctcga
ggcgcgccactagattaattaactcgagtcctaaagaagtcggcttctccatcggatcctgatcaggggagctcacggg
gacagcccccccccaaagcccccagggatgtaattacgtccctcccccgctagggggcagcagcgaccgcccggggctc
cgctccggtccggcgctccccccgcatcccgagccggcagcgtgcggggacagcccgggcacggggaaggtggcacggg
atcgctttcctctgaacgcttctcgctgctctttgagcctgcaaacacctgggggatacggggaaaatgatcaagatct
tctacactgggacgcaaggattatcctcgaggcgcgcc