LOCUS BBa_J176170 8320 bp DNA linear 29-Nov-2024 DEFINITION miR-34 CDK6 sensor 2.0 ACCESSION BBa_J176170 VERSION BBa_J176170.1 FEATURES Location/Qualifiers misc_feature 253..253 /label=mutated SpeI misc_feature 511..511 /label=mutated PstI promoter 1..516 /label=BBa_J176028 misc_feature 517..522 /label=BBa_J176041 misc_feature 523..539 /label=Gal4 misc_feature 542..558 /label=Gal4 misc_feature 561..577 /label=Gal4 misc_feature 580..596 /label=Gal4 misc_feature 599..615 /label=Gal4 misc_feature 523..615 /label=BBa_J176019 misc_feature 616..621 /label=BBa_J176041 misc_feature 622..638 /label=Gal4 misc_feature 641..657 /label=Gal4 misc_feature 660..676 /label=Gal4 misc_feature 679..695 /label=Gal4 misc_feature 698..714 /label=Gal4 misc_feature 622..714 /label=BBa_J176019 misc_feature 1..714 /label=BBa_S04655 misc_feature 715..720 /label=BBa_J176041 RBS 721..738 /label=BBa_J176012 misc_feature 739..744 /label=BBa_J176041 misc_feature 745..1278 /label=BBa_J176018 misc_feature 1279..1284 /label=BBa_J176041 misc_feature 1285..1971 /label=BBa_J176022 misc_feature 1972..1977 /label=BBa_J176041 misc_feature 1978..2664 /label=BBa_J176022 misc_feature 2665..2670 /label=BBa_J176041 misc_feature 2671..2691 /label=BBa_J176008 misc_feature 2692..2697 /label=BBa_J176041 terminator 2698..2710 /label=BBa_J176016 misc_feature 2711..2716 /label=BBa_J176041 misc_feature 2844..2844 /label=questionable base call misc_feature 2717..3184 /label=BBa_J176042 misc_feature 721..3184 /label=BBa_S04704 misc_feature 3185..3190 /label=BBa_J176041 misc_feature 3191..3530 /label=BBa_J176021 misc_feature 3531..3536 /label=BBa_J176041 misc_feature 3537..3876 /label=BBa_J176021 misc_feature 3877..3882 /label=BBa_J176041 misc_feature 4135..4135 /label=mutated SpeI misc_feature 4393..4393 /label=mutated PstI promoter 3883..4398 /label=BBa_J176028 misc_feature 4399..4404 /label=BBa_J176041 RBS 4405..4422 /label=BBa_J176012 misc_feature 4423..4428 /label=BBa_J176041 misc_feature 4429..4887 /label=BBa_J176020 misc_feature 4888..4893 /label=BBa_J176041 misc_feature 4894..5427 /label=BBa_J176018 misc_feature 5428..5433 /label=BBa_J176041 misc_feature 5434..6138 /label=BBa_J176005 misc_feature 6139..6144 /label=BBa_J176041 misc_feature 6145..6849 /label=BBa_J176005 misc_feature 6850..6855 /label=BBa_J176041 misc_feature 6856..6876 /label=BBa_J176008 misc_feature 6877..6882 /label=BBa_J176041 terminator 6883..6895 /label=BBa_J176016 misc_feature 3191..6895 /label=BBa_S04700 misc_feature 6896..6901 /label=BBa_J176041 misc_feature 6902..6921 /label=CDK6 miR-34 target misc_feature 6922..6941 /label=CDK6 miR-34 target misc_feature 6942..6961 /label=CDK6 miR-34 target misc_feature 6902..6961 /label=BBa_J176135 misc_feature 6962..6967 /label=BBa_J176041 misc_feature 6995..7013 /label=repeat 1 misc_feature 7022..7040 /label=repeat 2 misc_feature 7049..7067 /label=repeat 3 misc_feature 7076..7094 /label=repeat 4 misc_feature 7102..7120 /label=repeat 5 misc_feature 6968..7154 /label=BBa_J176025 misc_feature 7155..7160 /label=BBa_J176041 misc_feature 7288..7288 /label=questionable base call misc_feature 7161..7628 /label=BBa_J176042 misc_feature 7629..7634 /label=BBa_J176041 misc_feature 7635..7974 /label=BBa_J176021 misc_feature 7975..7980 /label=BBa_J176041 misc_feature 7981..8320 /label=BBa_J176021 misc_feature 6968..8320 /label=BBa_S04691 ORIGIN 1 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 61 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 241 agcacgtctc acaagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 481 ctcttcctca tctccgggcc tttcgacctg gagcccacta gacggagtac tgtcctccga 541 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg 601 gagtactgtc ctccgactag acggagtact gtcctccgag cggagtactg tcctccgagc 661 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgactaga 721 cccgccgcca ccatggagac tagagcatcc aacttgaaca tttcgagaaa gcttaccata 781 tcaaccccat catgctcttt cgaaaattca aatagcacat ccattccttc gcccgcttcc 841 tcatctcaaa gccacactcc aatgagaaac atgagctcac tctctgataa cagcgttttc 901 agccggaata tggaacaatc atcaccaatc actccaagta tgtaccaatt tggtcagcag 961 cagtcaaaca gtatatgtgg tagcaccgtt agtgtgaata gtctggtgaa tacaaataac 1021 aaacaaagga tctacgaaca aatcacgggt cctaacagca ataacgcaac caatgattat 1081 attgatttgc taaacctaaa tgagtctaac aaggaaaacc aaaatcccgc aacggcgcat 1141 tacctcaatg ggggcccacc caagacaagc ttcattaacc atggaatgtt cccctcgcca 1201 actgggacca taaatagcgg taaatctagc agtgcctcat ctttaatttc ttttggtatg 1261 ggcaataccc aagtaataac tagaatggcc ctgtccaaca agttcatcgg cgacgacatg 1321 aagatgacct accacatgga cggctgcgtg aacggccact acttcaccgt gaagggcgag 1381 ggcagcggca agccctacga gggcacccag acctccacct tcaaggtgac catggccaac 1441 ggcggccccc tggccttctc cttcgacatc ctgtccaccg tgttcatgta cggcaaccgc 1501 tgcttcaccg cctaccccac cagcatgccc gactacttca agcaggcctt ccccgacggc 1561 atgtcctacg agagaacctt cacctacgag gacggcggcg tggccaccgc cagctgggag 1621 atcagcctga agggcaactg cttcgagcac aagtccacct tccacggcgt gaacttcccc 1681 gccgacggcc ccgtgatggc caagaagacc accggctggg acccctcctt cgagaagatg 1741 accgtgtgcg acggcatctt gaagggcgac gtgaccgcct tcctgatgct gcaaggcggc 1801 ggcaactaca gatgccagtt ccacacctcc tacaagacca agaagcccgt gaccatgccc 1861 cccaaccacg tggtggagca ccgcatcgcc agaaccgacc tggacaaggg cggcaacagc 1921 gtgcagctga ccgagcacgc cgtggcccac atcacctccg tggtgccctt cactagaatg 1981 gccctgtcca acaagttcat cggcgacgac atgaagatga cctaccacat ggacggctgc 2041 gtgaacggcc actacttcac cgtgaagggc gagggcagcg gcaagcccta cgagggcacc 2101 cagacctcca ccttcaaggt gaccatggcc aacggcggcc ccctggcctt ctccttcgac 2161 atcctgtcca ccgtgttcat gtacggcaac cgctgcttca ccgcctaccc caccagcatg 2221 cccgactact tcaagcaggc cttccccgac ggcatgtcct acgagagaac cttcacctac 2281 gaggacggcg gcgtggccac cgccagctgg gagatcagcc tgaagggcaa ctgcttcgag 2341 cacaagtcca ccttccacgg cgtgaacttc cccgccgacg gccccgtgat ggccaagaag 2401 accaccggct gggacccctc cttcgagaag atgaccgtgt gcgacggcat cttgaagggc 2461 gacgtgaccg ccttcctgat gctgcaaggc ggcggcaact acagatgcca gttccacacc 2521 tcctacaaga ccaagaagcc cgtgaccatg ccccccaacc acgtggtgga gcaccgcatc 2581 gccagaaccg acctggacaa gggcggcaac agcgtgcagc tgaccgagca cgccgtggcc 2641 cacatcacct ccgtggtgcc cttcactaga cccaagaaaa agcgcaaggt aactagagcg 2701 taaagctgag actagaagac atgataagat acattgatga gtttggacaa accacaacta 2761 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa 2821 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg 2881 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg 2941 ctgattatga tcctgcaagc ctcgtcgtcc tggccggacc acgctatctg tgcaaggtcc 3001 ccggccccgg acgcgcgctc catgagcaga gcgcccgccg ccgaggcgaa gactcgggcg 3061 gcgccctgcc cgtcccacca ggtcaacagg cggtaaccgg cctcttcatc gggaatgcgc 3121 gcgaccttca gcatcgccgg catgtccccc tggcggacgg gaagtatcca gctcgaccaa 3181 gcttactaga ttaattaact cgagtcctaa agaagtcggc ttctccatcg gatcctgatc 3241 aggggagctc acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc 3301 cccgctaggg ggcagcagcg accgcccggg gctccgctcc ggtccggcgc tccccccgca 3361 tcccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt 3421 tcctctgaac gcttctcgct gctctttgag cctgcaaaca cctgggggat acggggaaaa 3481 tgatcaagat cttctacact gggacgcaag gattatcctc gaggcgcgcc actagattaa 3541 ttaactcgag tcctaaagaa gtcggcttct ccatcggatc ctgatcaggg gagctcacgg 3601 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca 3661 gcagcgaccg cccggggctc cgctccggtc cggcgctccc cccgcatccc gagccggcag 3721 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt 3781 ctcgctgctc tttgagcctg caaacacctg ggggatacgg ggaaaatgat caagatcttc 3841 tacactggga cgcaaggatt atcctcgagg cgcgccacta gactaccggg taggggaggc 3901 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta 3961 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc 4021 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc 4081 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcacaagtct 4141 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca 4201 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg 4261 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc 4321 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg 4381 cctttcgacc tggagcccac tagacccgcc gccaccatgg agactagaat gaagctactg 4441 tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa 4501 aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc 4561 aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg 4621 gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat 4681 tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa 4741 gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag 4801 catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg 4861 actgtatcgc cggaatttcc ggggatcact agagcatcca acttgaacat ttcgagaaag 4921 cttaccatat caaccccatc atgctctttc gaaaattcaa atagcacatc cattccttcg 4981 cccgcttcct catctcaaag ccacactcca atgagaaaca tgagctcact ctctgataac 5041 agcgttttca gccggaatat ggaacaatca tcaccaatca ctccaagtat gtaccaattt 5101 ggtcagcagc agtcaaacag tatatgtggt agcaccgtta gtgtgaatag tctggtgaat 5161 acaaataaca aacaaaggat ctacgaacaa atcacgggtc ctaacagcaa taacgcaacc 5221 aatgattata ttgatttgct aaacctaaat gagtctaaca aggaaaacca aaatcccgca 5281 acggcgcatt acctcaatgg gggcccaccc aagacaagct tcattaacca tggaatgttc 5341 ccctcgccaa ctgggaccat aaatagcggt aaatctagca gtgcctcatc tttaatttct 5401 tttggtatgg gcaataccca agtaataact agagtgagca agggcgagga ggataacatg 5461 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac 5521 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag 5581 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc 5641 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg 5701 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg 5761 accgtgaccc aggactcctc cttgcaggac ggcgagttca tctacaaggt gaagctgcgc 5821 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc 5881 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg 5941 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag 6001 cccgtgcagc tacccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac 6061 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc 6121 atggacgagc tgtacaagac tagagtgagc aagggcgagg aggataacat ggccatcatc 6181 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag 6241 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg 6301 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc 6361 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc 6421 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc 6481 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac 6541 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag 6601 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag 6661 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag 6721 ctacccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac 6781 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag 6841 ctgtacaaga ctagacccaa gaaaaagcgc aaggtaacta gagcgtaaag ctgagactag 6901 acagtgtgga aattcactgc ccagtgtgga aattcactgc ccagtgtgga aattcactgc 6961 cactagaata gttgtttaaa cgagctcgct agcaaatcaa gagtcctcat aaaggaatta 7021 aaatcaagag tcctcataaa gtctagcaaa tcaagagtcc tcataaagct agacaaatca 7081 agagtcctca taaataattc caatcaagag tcctcataaa gtcgacctgc aagcatgcaa 7141 gctgatccgg ctgcactaga agacatgata agatacattg atgagtttgg acaaaccaca 7201 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt 7261 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt 7321 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt 7381 atggctgatt atgatcctgc aagcctcgtc gtcctggccg gaccacgcta tctgtgcaag 7441 gtccccggcc ccggacgcgc gctccatgag cagagcgccc gccgccgagg cgaagactcg 7501 ggcggcgccc tgcccgtccc accaggtcaa caggcggtaa ccggcctctt catcgggaat 7561 gcgcgcgacc ttcagcatcg ccggcatgtc cccctggcgg acgggaagta tccagctcga 7621 ccaagcttac tagattaatt aactcgagtc ctaaagaagt cggcttctcc atcggatcct 7681 gatcagggga gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc 7741 ctcccccgct agggggcagc agcgaccgcc cggggctccg ctccggtccg gcgctccccc 7801 cgcatcccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc 7861 gctttcctct gaacgcttct cgctgctctt tgagcctgca aacacctggg ggatacgggg 7921 aaaatgatca agatcttcta cactgggacg caaggattat cctcgaggcg cgccactaga 7981 ttaattaact cgagtcctaa agaagtcggc ttctccatcg gatcctgatc aggggagctc 8041 acggggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg 8101 ggcagcagcg accgcccggg gctccgctcc ggtccggcgc tccccccgca tcccgagccg 8161 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac 8221 gcttctcgct gctctttgag cctgcaaaca cctgggggat acggggaaaa tgatcaagat 8281 cttctacact gggacgcaag gattatcctc gaggcgcgcc //