LOCUS BBa_J176171 4611 bp DNA linear 24-Nov-2024 DEFINITION Kozak-XbaI-NLS-6His-stop ACCESSION BBa_J176171 VERSION BBa_J176171.1 FEATURES Location/Qualifiers primer_bind 186..203 /label=DD122 B misc_feature 232..249 /label=pCMV remnant misc_feature 250..304 /label=MCS: SpeI BstXI EcoRI PstI EcoRV BstXI NotI XhoI RBS 311..328 /label=Kozak misc_feature 329..334 /label=XbaI misc_feature 335..355 /label=NLS misc_feature 356..373 /label=6-His misc_feature 374..386 /label=A - Stop - S - stop primer_bind 418..435 /label=BGH reverse primer_bind 426..444 /label=DD123 B polyA_site 424..648 /label=BGH polyA misc_feature 694..1122 /label=f1 origin promoter 1127..1471 /label=SV40 promoter/ origin CDS 1475..2074 /label=Puromycin R polyA_site 2288..2417 /label=SV40 polyA CDS 3615..4475 /label=Ampicillin R promoter 4476..4574 /label=bla promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgct 301 cgagtctagt cccgccgcca ccatggagtc tagacccaag aaaaagcgca aggtacacca 361 tcaccaccat cacgcgtaaa gctgagacta gagggcccgt ttaaacccgc tgatcagcct 421 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 481 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 541 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 601 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 661 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 721 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 781 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 841 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 901 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 961 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 1021 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 1081 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 1141 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 1201 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 1261 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 1321 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 1381 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 1441 ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc ccacggtgcg 1501 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 1561 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 1621 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 1681 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 1741 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 1801 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 1861 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 1921 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 1981 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gagtgcccga aggaccgcgc 2041 gacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc 2101 gaccgaaagg agcgcacgac cccatgcatc gaaatgaccg accaagcgac gcccaacctg 2161 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 2221 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 2281 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 2341 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 2401 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 2461 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 2521 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 2581 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 2641 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 2701 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 2761 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 2821 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 2881 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 2941 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3001 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3061 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3121 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3181 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3241 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 3301 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3361 tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt 3421 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 3481 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 3541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 3601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 3661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 3721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 3781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 3841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 3901 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 3961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 4021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 4081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 4141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 4201 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 4261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 4321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 4381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 4441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 4501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 4561 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c //