LOCUS       BBa_J176176              986 bp    DNA     linear       26-Nov-2024
DEFINITION  GFP reporter with O2 operator
ACCESSION   BBa_J176176
VERSION     BBa_J176176.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    44..64
                     /label=BBa_J176173
     misc_feature    77..80
                     /label=conserved
     RBS             73..84
                     /label=BBa_B0034
     misc_feature    91..810
                     /label=BBa_E0040
     misc_feature    805..843
                     /label=LVA
     misc_feature    844..849
                     /label=Double Stop
     misc_feature    91..93
                     /label=Start
     misc_feature    91..849
                     /label=BBa_J04031
     stem_loop       869..912
                     /label=stem_loop
     misc_feature    858..937
                     /label=BBa_B0010
     terminator      858..937
                     /label=BBa_B0010
     stem_loop       953..972
                     /label=T7 TE
     misc_feature    979..979
                     /label=stop
     polyA_site      973..986
                     /label=polya
     misc_feature    946..986
                     /label=BBa_B0012
     terminator      946..986
                     /label=BBa_B0012
     terminator      858..986
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaatgtg agcgagtaac
       61 aacctactag agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
      121 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      181 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      241 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      301 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      361 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      421 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      481 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      541 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      601 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      661 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      721 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      781 catggcatgg atgaactata caaaaggcct gctgcaaacg acgaaaacta cgctttagta
      841 gcttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      901 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      961 ttcgggtggg cctttctgcg tttata
//