LOCUS       BBa_J176178             1380 bp    DNA     linear       25-Nov-2024
DEFINITION  LacI Repressor with O2 Operator
ACCESSION   BBa_J176178
VERSION     BBa_J176178.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     misc_feature    44..64
                     /label=BBa_J176173
     misc_feature    77..80
                     /label=conserved
     RBS             73..84
                     /label=BBa_B0034
     misc_feature    1180..1218
                     /label=LVA
     CDS             91..1218
                     /label=lacI-LVA
     misc_feature    91..1218
                     /label=BBa_C0012
     misc_feature    1219..1243
                     /label=Help:Barcodes
     CDS             91..1218
                     /label=BBa_C0012
     stem_loop       1263..1306
                     /label=stem_loop
     misc_feature    1252..1331
                     /label=BBa_B0010
     terminator      1252..1331
                     /label=BBa_B0010
     stem_loop       1347..1366
                     /label=T7 TE
     misc_feature    1373..1373
                     /label=stop
     polyA_site      1367..1380
                     /label=polya
     misc_feature    1340..1380
                     /label=BBa_B0012
     terminator      1340..1380
                     /label=BBa_B0012
     terminator      1252..1380
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaatgtg agcgagtaac
       61 aacctactag agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac
      121 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
      181 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
      241 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
      301 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
      361 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
      421 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
      481 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
      541 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
      601 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
      661 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
      721 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
      781 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
      841 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
      901 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
      961 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     1021 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     1081 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1141 cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac
     1201 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gccaggcatc
     1261 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1321 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//