LOCUS BBa_J179000 6105 bp DNA linear 22-Nov-2024 DEFINITION pBS1C: Bacillus subtilis vector, amyE integration, CAM-resistance ACCESSION BBa_J179000 VERSION BBa_J179000.1 FEATURES Location/Qualifiers CDS 358..1008 /label=cat misc_feature 1379..2407 /label=amyE-back CDS 4361..5221 /label=bla misc_feature 5546..6066 /label=amyE-front misc_feature 3587..4201 /label=ori ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaattc tcatgtttga 61 cagcttatca tcggcaatag ttacccttat tatcaagata agaaagaaaa ggatttttcg 121 ctacgctcaa atcctttaaa aaaacacaaa agaccacatt ttttaatgtg gtctttattc 181 ttcaactaaa gcacccatta gttcaacaaa cgaaaattgg ataaagtggg atatttttaa 241 aatatatatt tatgttacag taatattgac ttttaaaaaa ggattgattc taatgaagaa 301 agcagacaag taagcctcct aaattcactt tagataaaaa tttaggaggc atatcaaatg 361 aactttaata aaattgattt agacaattgg aagagaaaag agatatttaa tcattatttg 421 aaccaacaaa cgacttttag tataaccaca gaaattgata ttagtgtttt ataccgaaac 481 ataaaacaag aaggatataa attttaccct gcatttattt tcttagtgac aagggtgata 541 aactcaaata cagcttttag aactggttac aatagcgacg gagagttagg ttattgggat 601 aagttagagc cactttatac aatttttgat ggtgtatcta aaacattctc tggtatttgg 661 actcctgtaa agaatgactt caaagagttt tatgatttat acctttctga tgtagagaaa 721 tataatggtt cggggaaatt gtttcccaaa acacctatac ctgaaaatgc tttttctctt 781 tctattattc catggacttc atttactggg tttaacttaa atatcaataa taatagtaat 841 taccttctac ccattattac agcaggaaaa ttcattaata aaggtaattc aatatattta 901 ccgctatctt tacaggtaca tcattctgtt tgtgatggtt atcatgcagg attgtttatg 961 aactctattc aggaattgtc agataggcct aatgactggc ttttataata tgagataatg 1021 ccgactgtac tttttacagt cggttttcta atgtcactaa cctgccccgt tagttgaaga 1081 aggtttttat attacagctc cagatcctct acgccggacg catcgtggca ggcatcaccg 1141 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg 1201 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 1261 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca 1321 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc 1381 gacatggatg agcgatgatg atatccgttt aggctgggcg gtgatagctt ctcgttcagg 1441 cagtacgcct cttttctttt ccagacctga gggaggcgga aatggtgtga ggttcccggg 1501 gaaaagccaa ataggcgatc gcgggagtgc tttatttgaa gatcaggcta tcactgcggt 1561 caatagattt cacaatgtga tggctggaca gcctgaggaa ctctcgaacc cgaatggaaa 1621 caaccagata tttatgaatc agcgcggctc acatggcgtt gtgctggcaa atgcaggttc 1681 atcctctgtc tctatcaata cggcaacaaa attgcctgat ggcaggtatg acaataaagc 1741 tggagcgggt tcatttcaag tgaacgatgg taaactgaca ggcacgatca atgccaggtc 1801 tgtagctgtg ctttatcctg atgatattgc aaaagcgcct catgttttcc ttgagaatta 1861 caaaacaggt gtaacacatt ctttcaatga tcaactgacg attaccttgc gtgcagatgc 1921 gaatacaaca aaagccgttt atcaaatcaa taatggacca gacgacaggc gtttaaggat 1981 ggagatcaat tcacaatcgg aaaaggagat ccaatttggc aaaacataca ccatcatgtt 2041 aaaaggaacg aacagtgatg gtgtaacgag gaccgagaaa tacagttttg ttaaaagaga 2101 tccagcgtcg gccaaaacca tcggctatca aaatccgaat cattggagcc aggtaaatgc 2161 ttatatctat aaacatgatg ggagccgagt aattgaattg accggatctt ggcctggaaa 2221 accaatgact aaaaatgcag acggaattta cacgctgacg ctgcctgcgg acacggatac 2281 aaccaacgca aaagtgattt ttaataatgg cagcgcccaa gtgcccggtc agaatcagcc 2341 tggctttgat tacgtgctaa atggtttata taatgactcg ggcttaagcg gttctcttcc 2401 ccattgaggg caaggctaga cgggacttac cgaaagaaac catcaatgat ggtttctttt 2461 ttgttcataa atcagacaaa acttttctct tgcaaaagtt tgtgaagtgt tgcacaatat 2521 aaatgtgaaa tacttcacaa acaaaaagac atcaaagaga aacataccct gcaaggatgc 2581 tgatattgtc tgcatttgcg ccggagcaaa ccaaaaacct ggtgagacac gccttgaatt 2641 agtagaaaag aacttgaaga ttttcaaagg catcgttagt gaagtcatgg cgagcggatt 2701 tgacggcatt ttcttagtcg cgacgcgagg ctggatggcc ttccccatta tgattcttct 2761 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga 2821 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac 2881 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 2941 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 3001 gagccgggcc acctactgaa gtggatttct ttaagagctc ctttaacttc ctcaccagta 3061 gttgtatcgg taccataagt agaagcagca acccaagtag ctttaccagc atccggttca 3121 accagcatag taagaatctt actggacatc ggcagttctt cgaacagtgc gccaactacc 3181 agctctttct ccagaatggg ctatacctct tttacctaaa gagcatgtaa ctttactgga 3241 tatagctaga aaaggctatc ggggagagtg tgatgataag tgggaaggac tatattcaaa 3301 ggtgaaagca ctcgttaagt atatgaaaaa ttctatagaa acttctctca attaggctaa 3361 ttttattgca ataacaggtg cttacttttc tggagttctt tagcaaattt ttttattagc 3421 tgaacttagt attagtggcc atactcctcc aatccaaagc tatttagaaa gattactata 3481 tcctcaaaca ggcggtaacc ggcctcttca tcgggaatgc gcgcgacctt cagcatcacc 3541 ggcatgtccc cctggcggac gggaagtatc cagctcgagg tcgggccgcg ttgctggcgt 3601 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 3661 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 3721 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 3781 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 3841 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 3901 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 3961 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 4021 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 4081 ccttcggaaa aagagttgat agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 4141 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4201 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4261 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 4321 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4381 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4441 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 4501 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4561 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4621 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgctg 4681 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 4741 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 4801 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 4861 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 4921 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac 4981 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 5041 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 5101 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5161 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5221 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5281 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5341 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 5401 gtatcacgag gccctttcgt cttcaagaat taacaaaatt ctccagtctt cacatcggtt 5461 tgaaaggagg aagcggaaga atgaagtaag agggattttt gactccgaag taagtcttca 5521 aaaaatcaaa taaggagtgt caagaatgtt tgcaaaacga ttcaaaacct ctttactgcc 5581 gttattcgct ggatttttat tgctgtttca tttggttctg gcaggaccgg cggctgcgag 5641 tgctgaaacg gcgaacaaat cgaatgagct tacagcaccg tcgatcaaaa gcggaaccat 5701 tcttcatgca tggaattggt cgttcaatac gttaaaacac aatatgaagg atattcatga 5761 tgcaggatat acagccattc agacatctcc gattaaccaa gtaaaggaag ggaatcaagg 5821 agataaaagc atgtcgaact ggtactggct gtatcagccg acatcgtatc aaattggcaa 5881 ccgttactta ggtactgaac aagaatttaa agaaatgtgt gcagccgctg aagaatatgg 5941 cataaaggtc attgttgacg cggtcatcaa tcataccacc agtgattatg ccgcgatttc 6001 caatgaggtt aagagtattc caaactggac acatggaaac acacaaatta aaaactggtc 6061 tgatcggatc ctagaagctt atcgaattcg cggccgcttc tagag //