LOCUS BBa_J179001 6258 bp DNA linear 22-Nov-2024 DEFINITION pBS2E: Bacillus subtilis vector, lacA integration, MLS resistance ACCESSION BBa_J179001 VERSION BBa_J179001.1 FEATURES Location/Qualifiers misc_feature 124..531 /label=lacA back CDS 747..1607 /label=bla misc_feature 1758..2381 /label=ori misc_feature 4078..4593 /label=lacA front CDS 4891..5628 /label=erm ORIGIN 1 tactagtagc ggccgctgca gagatatcga tttcaagcta tatttggagt tgagcctctt 61 gaaacggaca ccctgtatcc gaaggatcga aacgctgtca gctaccgcag ccaaatatat 121 gaaatgaagg attatgcaac cgtgattgat gtaaagacag cttcagtgga agcggtgtat 181 caagaagatt tttatgcgcg cacgccagcg gtcacaagcc atgagtatca gcagggcaag 241 gcgtatttta tcggcgcgcg tttggaggat caatttcagc gtgatttcta tgagggtctg 301 atcacagacc tgtctctctc tccagttttt ccggttcggc acggaaaagg cgtctccgta 361 caagcgaggc aggatcagga caatgattat atttttgtca tgaatttcac ggaagaaaaa 421 cagctggtca cgtttgatca gagtgtgaag gacataatga caggagacat attgtcaggc 481 gacctgacga tggaaaagta tgaagtgaga attgtcgtaa acacacatta gcccatcatt 541 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 601 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 661 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 721 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 781 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 841 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 901 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 961 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc 1021 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 1081 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 1141 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 1201 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 1261 caaacgacga gcgtgacacc acgatgccag cagcaatggc aacaacgttg cgcaaactat 1321 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 1381 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 1441 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 1501 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 1561 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 1621 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 1681 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 1741 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 1801 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 1861 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 1921 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 1981 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 2041 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 2101 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 2161 agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 2221 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 2281 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 2341 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 2401 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2461 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 2521 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 2581 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 2641 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 2701 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 2761 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 2821 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc 2881 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct 2941 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcacttgat gcctccgtgt 3001 aagggggaat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg 3061 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg 3121 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt 3181 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac 3241 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag 3301 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc 3361 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc 3421 ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg 3481 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt 3541 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg 3601 ttagcgaggt gccgccagct tccattcagg tcgaggtggc ccggctccat gcaccgcgac 3661 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc 3721 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta 3781 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc 3841 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa 3901 tggggaaggc catccagcct cgcgtcgcga ctaagaaaat gccgtcaaat ccgctcgcca 3961 tgacttcact aacgatgcct ttgaaaatct tcaagttctt ttctactaat tcaaggcgtg 4021 tctcaccagg tttttggttt gctccggcgc aaatgcagac aatatcagga tggggtgatg 4081 tcaaagcttg aaaaaacgca cgtaacaaaa gcaaaattta tgctccatgg gggagactac 4141 aaccccgatc agtggctgga tcggcccgat attttagctg acgatatcaa actgatgaag 4201 ctttctcata cgaatacgtt ttctgtcggc atttttgcat ggagcgcact tgagccggag 4261 gagggcgtat atcaatttga atggctggat gatatttttg agcggattca cagtataggc 4321 ggccgggtca tattagcaac gccgagcgga gcccgtccgg cctggctgtc gcaaacctat 4381 ccggaagttt tgcgcgtcaa tgcctcccgc gtcaaacagc tgcacggcgg aaggcacaac 4441 cactgcctca catctaaagt ctaccgagaa aaaacacggc acatcaaccg cttattagca 4501 gaacgatacg gacatcaccc ggcgctgtta atgtggcaca tttcaaacga atacggggga 4561 gattgccact gtgaatcgat gcggccgctc taggagttaa caagagtttg tagaaacgca 4621 aaaaggccat ccgtcaggat ggccttctgc ttagctagag cggcggattt gtcctactca 4681 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct 4741 ttcgttttat ttgatgcctc aagctagaga gtcctagagt ctagggacct ctttagctcc 4801 ttggaagctg tcagtagtat acctaataat ttatctacat tccctttagt aacgtgtaac 4861 tttccaaatt tacaaaagcg actcatagaa ttatttcctc ccgttaaata atagataact 4921 attaaaaata gacaatactt gctcataagt aacggtactt aaattgttta ctttggcgtg 4981 tttcattgct tgatgaaact gatttttagt aaacagttga cgatattctc gattgaccca 5041 ttttgaaaca aagtacgtat atagcttcca atatttatct ggaacatctg tggtatggcg 5101 ggtaagtttt attaagacac tgtttacttt tggtttagga tgaaagcatt ccgctggcag 5161 cttaagcaat tgctgaatcg agacttgagt gtgcaagagc aaccctagtg ttcggtgaat 5221 atccaaggta cgcttgtaga atccttcttc aacaatcaga tagatgtcag acgcatggct 5281 ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg taaggaatac tcccaacaat 5341 tttatacctc tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa ttaaagtgac 5401 acgagtattc agttttaatt tttctgacga taagttgaat agatgactgt ctaattcaat 5461 agacgttacc tgtttactta ttttagccag tttcgtcgtt aaatgccctt tacctgttcc 5521 aatttcgtaa acggtatcgg tttcttttaa attcaattgt tttattattt ggttgagtac 5581 tttttcactc gttaaaaagt tttgagaata ttttatattt ttgttcatgt aatcactcct 5641 tcttaattac aaatttttag catctaattt aacttcaatt cctattatac aaaattttaa 5701 gatactgcac tatcaacaca ctcttaagtt tgcttctaag tcttatttcc ataacttctt 5761 ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag tcagcacgaa 5821 cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta tcatttcgtt 5881 tttcttttgt gctagaggat caattcttga agacgaaagg gcctcgtgat acgcctattt 5941 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 6001 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 6061 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tgcggccgcc 6121 cgcgggagct ccccgggacg ttcttgccat tgctgcataa aaaacgcccg gcggcaaccg 6181 agcgttctga attaattaat catcgcgact gcatctttcg ctaaggatga tttctggaat 6241 tcgcggccgc ttctagag //